ACTIVITY OF POLYHERBAL EXTRACT AGAINST BACTERIA CAUSING SKIN INFECTIONS IN DIABETIC PATIENTS.

Abstract

Objective   : - The objective of   this   study is to evaluate antibacterial activity of Polyherbal extract on few    microorganisms causing skin infections in most of the  diabetic patients  .

Methods   : - Methanolic Polyherbal extract was prepared  from Acacia ferruginia (bark & fruit), Chloroxylon swietenia (bark, leaf, root), Casearia elliptica (bark), Terminalia alata (root, leaf). The preliminary phytochemical analysis was performed to identify the major phytoconstituents  in the extract.  Polyherbal extract was evaluated for antibacterial activity against bacteria   like Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa,   Escherichia coli, Proteus species, Klebsiella species causing skin infections , diabetic foot infections in diabetic patients. In-vitro method of agar diffusion disc method was used for the evaluation of antibacterial activity through zone of inhibition.  The standard drug used was Gentamicin. Minimum Inhibitory Concentration (MIC) of the extract was done  using broth dilution method.

Results:  The polyherbal extract showed good antibacterial activity. The standard drug used was Gentamicin.  The Methanolic   Polyherbal extract showed   potent activityon all the selected microorganisms. The Polyherbal extract was active  against Pseudomonas aeruginosa (2.57 +  0.39) and E.coli (2.92 + 0.29) when compared with standard. The polyherbal extract showed potent activity  on Bacillus subtilis and  Proteus vulgaris.

Conclusion : The polyherbal extract showed broad spectrum activity by being active against gram positive and gram negative microorganisms providing the scope for further analysis as a antibacterial preparation for the skin infections in diabetes patients.