ASSESSMENT OF EXTRAPULMONARY TOXICITY INDUCED BY CARBON NANOMATERIALS FOLLOWING INTRA-TRACHEAL INSTILLATION IN RATS
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i5.17152Keywords:
Alanine transaminase, Carbon nanomaterials, Creatinine, ToxicityAbstract
Objective: Carbon nanomaterials (CNMs) such as carbon nanofibres (CNFs), multi wall carbon nanotubes (MWCNTs) and carbon nanorods (CNRs) were found various industrial and commercial applications. The occupational exposure for these CNMs were also increased enormously. The present study evaluated the extrapulmonary toxicity induced by these CNMs.Â
Methods: The extrapulmonary toxicity was assessed following intratracheal instillation of test CNMs in rats after 1 day, 1 week, 1 month and 3 months post exposure periods by using serum biochemical parameters such as alanine transaminase (ALT) and creatinine using diagnostic assay kits. Further, the histopathological analysis was performed for liver and kidneys of particle exposed rats.
Results: The results have displayed that increased levels of serum ALT and creatinine were found after 1 day, 1 week and 1 month post exposure periods indicating liver and kidney toxicity respectively. This toxicity was further confirmed by the changes observed in the histopathological analysis of rat liver and kidneys.
Conclusion: The CNFs, MWCNTs and CNRs able to translocate from the lungs into other extrapulmonary organs such as liver and kidney, and also cause dose dependent toxicity to them.
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Oberdorster E. Manufactured nanomaterials (Fullerenes, C60) induce oxidative stress in the brain of juvenile largemouth bass. Environ Health Perspect 2004;112:1058–62.
Oberdorster G, Oberdorster E, Oberdorster J. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect 2005;113:823–39.
Hoet PHM, Bruske-Hohlfeld I, Salata OV. Nanoparticles - known and unkown health risks. J Nanobiotech 2004;2:12-27.
Deloncle R, Guillard O, Huguet F. Modification of the blood-brain barrier through chronic intoxication by aluminum glutamate. Possible role in the etiology of Alzheimer's disease. Biol Trace Elem Res 1995;47:227-33.
Harikiran L, Narsimhareddy Y. Cytotoxicity, oxidative stress, and inflammation in human Hep G2 liver epithelial cells following exposure to gold nanorods. Toxicol Mech Meth 2016;26(5):340-47.
Harikiran L, Bhikku A, Narsimha RY. In vitro cytotoxicity of gold and silver nanorods using different human cell lines. Lat Am J Pharm 2015;34(7):1277-82.
Gelli K, Porika M, Anreddy RN. Assessment of pulmonary toxicity of MgO nanoparticles in rats. Environ Toxicol 2013;30:308-14.
Warheit DB, Brock WJ, Lee KP, Webb TR, Reed KL. Comparative pulmonary toxicity inhalation and instillation and studies with different TiO2 particle formulations: impact of surface treatments on particle toxicity. Toxicol Sci 2005;88:514–24.
Warheit DB, Hansen JF, Yuen IS, Kelly DP, Snajdr S, Hartsky MA. Inhalation of high concentrations of low toxicity dusts in rats results in pulmonary and macrophage clearance impairments. Toxicol App Pharmacol 1997;145:10–22.
Nemmar A, Hoet PH, Vanquickenborne B. Passage of inhaled particles into the blood circulation in humans. Circulation 2002;105:411–14.
Nemmar A, Vanbilloen H, Hoylaerts MF. Passage of intratracheally instilled ultrafine particles from the lung into the systemic circulation in hamster. Am J Respir Crit Care Med 2001;164:1665–68.
Driscoll KE, Costa DL, Hatch G. Henderson L. Intratreacheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: uses and limitations. Toxicol Sci 2000;55:24-35.
Leong BK, Coombs JK, Sabaitis KT. Quantitative morphometric analysis of pulmonary deposition of aerosol particles inhaled via intratracheal nebulization, intratracheal instillation or nose-only inhalation in rats. J App Toxicol 1998;18:149–60.
Lam C, James JT, Latch JN. Pulmonary toxicity of simulated lunar and Martian dusts in mice. II. Biomarkers of acute responses after intratracheal instillation. Inhal Toxicol 2002;14:917–28.
Lam CW, James JT, McCluskey R. Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. Toxicol Sci 2004;77:126–34.
Warheit DB, Webb TR, Reed KL, Frerichs S, Sayes CM. Pulmonary toxicity study in rats with three forms of ultrafine- TiO2 particles: Differential responses related to surface properties. Toxicology 2007;230:90–104.
Reddy AR, Reddy YN, Krishna DR, Himabindu V. Pulmonary toxicity assessment of multiwalled carbon nanotubes in rats following intratracheal instillation. Environ Toxicol 2012;27:211-19.
Warheit DB, Laurence BR, Reed KL, Roach DH, Reynolds GAM, Webb TR. Comparative pulmonary toxicity assessment of single wall carbon nanotubes in rats. Toxicol Sci 2004;77:117–25.
Hamilton J, Zheqiong W, Somenath M, Hawl PS. Holian1 Effect of MWCNT size, carboxylation, and purification on in vitro and in vivo toxicity, inflammation and lung pathology. Part Fibre Toxicol 2013;10: 57-63.
Berry JP, Arnoux B, Stanislas G. A microanalytic study of particles transport across the alveoli: Role of blood platelets. Biomedicine 1977;27:354-57.
Brown JS, Zeman KL, Bennett WD. Ultrafine particle deposition and clearance in the healthy and obstructed lung. Am J Respir Crit Care Med 2002;166:1240-47.
Pekkanen J, Peters A, Hoek G. Particulate air pollution and risk of ST-segment depression during repeated submaximal exercise tests among subjects with coronary heart disease. The exposure and risk assessment for fine and ultrafine particles in ambient air study. Circulation 2002;106:933-38.
Robert RM, James FS, Ann FH. Distribution and fibrotic response following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol 2013;10:1-14.
Robert RM, James FS, Ann FH. Extrapulmonary transport of MWCNT following inhalation exposure. Part Fibre Toxicol 2013;10:1-5.
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