MULTIPLE ANTIBIOTIC RESISTANCE INDEXING OF NON- FERMENTING GRAM NEGATIVE BACILLI
Keywords:Multi-drug resistance, Multiple antibiotic resistance, Non-fermenters, Antibiotic susceptibility testing, Hospital acquired the infection
Objective: The multiple antibiotic resistance (MAR) indexing and finding Multidrug resistant (MDR) bacteria will help to indicate the origin from high risk of contamination where the antibiotics are often used. Hence this study was carried out to give the MAR index of non-fermenting Gram negative bacilli in a tertiary care hospital which would help our infection control team also.
Methods: Drug resistance was tested by Kirby bauer's disc diffusion method. MAR index was calculated using the formula, a/b (were a= number of antibiotics to which the organism was resistant and b= total number of antibiotics to which the organism was tested).
Results: Out of 240 Gram negative non-fermenters isolated, 117 (49%) strains were greater than 0.2 of MAR index, 95(81%) was from in-patient department. 73(62%) were hospitalized for more than 3 days, 44 (38%) was from surgery department. 49(42%) was wound specimen. Out of 117 multiple antibiotic resistant isolates 99 (85%) were MDR isolates.
Conclusion: 51% prevalence of isolates >0.2 MAR index shows that the source of contamination can still be brought up down by proper surveillance and management with proper usage ofÂ surface and skin disinfectants especially in surgery ward where the MAR index has indicated more usage of antibiotics
Kumar E, Usha K, Ramana BV, Chaudhury A, Sai Gopal DV. Plasmid profile and curing, biofilm detection among ESBL producing isolates of Acinetobacter species. Int J Pharm Pharm Sci 2016;8(4):39-48.
Samanta P, Gautam V, Thapar R, Ray P. Emerging resistance of non-fermenting gram negative bacilli in a tertiary care center. Indian J Pathol Microbiol 2011;54:666-7.
Okeke IN, Laxminarayan R, Bhutta ZA, Duse AG, Jenkins P, Oâ€™Brien TF, et al. Antimicrobial resistance in developing countries. Lancet Inf Dis 2005;5(8):481-3.
Joly-Guillou ML. Clinical impact and pathogenicity of Acinetobacter. Clin Microbiol Infect 2005;11(11):868-73.
Osundiya OO, Oladele RO, Oduyebo OO. Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. Afr J Clin Exp Microbiol 2013;14(3):164-8.
Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: Our worst nightmare? Clin Infect Dis 2002;34(5):634-40.
Paul S, Bezbaruah RL, Roy MK, Ghosh AC. Multiple antibiotic resistance (MAR) index and its reversion in Pseudomonas aeruginosa. Appl Microbiol 1997;24(3):169-71.
Olayinka BA, Onile A. Antibiotic susceptibility and plasmid pattern of Pseudomonas aeruginosa from the surgical unit of a University Teaching Hospital in North central Nigeria. Int J Med Med Sci 2009;1(3):79-83.
Kennedy DM, Wilbard SS. Antimicrobial resistance profiles of bacteria isolated from chicken droppings in Dar es Salaam. Int J Pharm Pharm Sci 2015;7(9):268-71.
Stark RP, Maki DG. Bacteriuria in the catheterized patient. What quantitative level of bacteriuria is relevant? N Engl J Med 1984;311(9):560-4.
Krumperman PH. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 1983;46(1):165-70.
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