AMELIORATING THE METABOLIC DISORDER IN STREPTOZOTOCIN-INDUCED DIABETIC RATS BY HAEMATOCOCCUS PLUVIALIS EXTRACT
Keywords:Haematococcus pluvialis, Streptozotocin, Type 2 diabetes, Asymmetric dimethylarginine, Von Willebrand factor, Lipoxygenases, Histopathological examination
Objective: The purpose of the current study is to evaluate the antidiabetic influence of Haematococcus pluvialis (H. pluvialis) ethanolic extract on streptozotocin (STZ)-induced rats.
Methods: This influence was assessed by measuring; asymmetric dimethylarginine (ADMA), von willebrand factor (vWf), 15-lipoxygenase (15-LOX), 5-lipoxygenase (5-LOX), epidermal growth factor (EGF) and lipoxin-A4 (LXA4). The histopathological examinations of cardiac and renal tissues were also performed.
Results: The presentedÂ results showed an elevation in the levels of ADMA, vWf, 15-LOXand 5-LOX with percentage increaseÂ 430.30, 77.54, 61.05 and 81.09 %, respectively in diabetic rats. However, the EGF and LXA4 levels were decreased significantly (44.17 and51.94%, respectively) compared to normal control rats. On the other hand, treatment of diabetic rats with H. pluvialis improved ADMA, vWf, 15-LOX, 5LOX, EGF and LXA4 levels with amelioration percentages; 269.69, 54.77, 55.78, 72.68, 53.39 and 56.58%, respectively. Additionally, the histopathological examination of diabetic cardiac tissue revealed congestion of myocardial blood vessel, vacuolation of the sarcoplasm of cardiac myocytes and focal necrosis of myocytes associated with inflammatory cells infiltration. Beside, vacuolation of epithelial lining of renal tubules and endothelial lining glomerular tuft as well as cystic dilatation of renal tubules were detected. However, cardiac tissueÂ and renal tubules of diabetic rats treated with H. pluvialis ethanolic extract (150 mg/kg b.wt.) showed normal cardiac myocytes, vacuolation of epithelial lining renal tubules and endothelial lining glomerular tuft, congestion of glomerular tuft and intertubular renal blood vessels.
Conclusion: These results confirmed the improving role of H. pluvialis in controlling type 2diabetes.
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