FREE RADICAL SCAVENGING ACTIVITY OF MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI

Yuvarani Thambidurai; Sudarsanam D; Habeeb Skm; Ashok Kumar T

doi:10.22159/ajpcr.2017.v10i6.18115

Authors

Yuvarani Thambidurai Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India.
Sudarsanam D Department of Biotechnology, D.G. Vaishnav College, Arumbakkam, Chennai - 600106, Tamil Nadu, India.
Habeeb Skm Department of Genetic Engineering, School of Bioengineering, SRM University, Chennai - 603 203, Tamil Nadu, India.
Ashok Kumar T Naturophathy and Yoga Therapist, Zero Error, Dharmapuri - 636 705, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i6.18115

Keywords:

2, Nil, 2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Antioxidant activity, Marine sponges

Abstract

Objective: The main focus of this study is to screen the marine sponges for potent free radical scavenging activity.

Methods: Various methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay are employed to ascertain the antioxidant properties of marine sponges namely Dysidea herbacea and Sigmadocia pumila.

Results: On analyzing, the result of ABTS assay D. herbacea and S. pumila exhibited almost equal antioxidant properties. While calculating the inhibitory concentration 50% value for DPPH assay, the Sample 1 and 2 has an IC of 655.49 and 826.739 Î¼l, respectively, and in FRAP assay, the Sample 1 and 2 has an IC of 67.587 and 74.57 Î¼g, respectively.

Conclusion: Overall from this assay, D. herbacea revealed slightly better antioxidant activity when compared to S. pumila, also which in future may serve as a better source to fight against various diseases

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Author Biography

Yuvarani Thambidurai, Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India.

Assistant Professor,

Department of Biotechnology,

D.G. Vaishnav College

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