ANTIHEPATITIS C VIRUS ACTIVITY OF INDONESIAN MAHOGANY (TOONA SURENI)

Achmad Fuad Hafid; Tutik Sri Wahyuni; Lidya Tumewu; Evhy Apryani; Adita Ayu Permanasari; Myrna Adianti; Chie Aoki Utsubo; Aty Widyawaruyanti; Maria Inge Lusida; Soetjipto Soetjipto; Hiroyuki Fuchino; Nobuo Kawahara; Hak Hotta

doi:10.22159/ajpcr.2018.v11i2.18126

Authors

Achmad Fuad Hafid Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Tutik Sri Wahyuni Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Lidya Tumewu Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Evhy Apryani Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Adita Ayu Permanasari Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Myrna Adianti Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Chie Aoki Utsubo Department of International Health, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe 654-0142, Japan.
Aty Widyawaruyanti Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Maria Inge Lusida Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Soetjipto Soetjipto Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Hiroyuki Fuchino Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan.
Nobuo Kawahara Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Tsukuba, Ibaraki, Japan.
Hak Hotta Department of Oral Vaccine and Drug Development, Kobe University Graduate School of Health Sciences, 1-5-6 Minatojima-minamimachi, Chou-ku, Kobe 650-0047, Japan.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.18126

Keywords:

Toona sureni, Leaves extract, Polyphenols, Antihepatitis C virus, Gallic acid

Abstract

Â Objective: Toona sureni (Indonesian mahogany) is a member of Meliaceae family and locally known as suren. Previous study reported that T. sureni leaves extract exhibited antiviral activity with 50% inhibitory concentration (IC50) value of 13.9 Â± 1.6 Î¼g/ml against hepatitis C virus (HCV) J6/JFH1. Cytotoxicity analysis of T. sureni leaves extract did not reveal any cytotoxicity effect; therefore, further study was taken to investigate the active substances from the extract.

Methods: Bioassay-guided isolation of anti-HCV was conducted using Huh-7.5 cells infected with HCV J6/JFH1 in the presence of extracts, fractions, or compounds from the plant.

Results: Ethyl acetate fraction (Fr E) exhibited high anti-HCV activity with IC50 value of 1.7 Î¼g/ml. Further, separation of Fr E by open column chromatography resulted in nine sub-fractions (sub-Fr E1-E9). Sub-Fr E3 and E4 have IC50 value of 29.90 Î¼g/ml and 7.68 Î¼g/ml, respectively. Polyphenols compounds have been isolated from sub-Fr E3 and E4. The structures have been determined to be ethyl gallate (1), methyl gallate (2), catechin (3), gallic acid (4), and quercetin 3-O-rhamnoside (5). Among the isolated compounds, gallic acid showed to possess strong anti-HCV activity with IC50 value of 15.9 Î¼g/ml.

Conclusion: T. sureni and its isolated compound, gallic acid, may be good candidates to develop for alternative and/or complementary agents of anti-HCV infection.

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Author Biography

Achmad Fuad Hafid, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.

Institute of Tropical Disease, Universitas Airlangga

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