IN VITRO EVALUATION OF ANTICANCER POTENTIAL OF ERYTHRINA VARIEGATA L. ON BREAST CANCER CELL LINES

Vaishali Rai M; Vinitha Ramanath Pai; Samuel Kevin; Herga P Kedilaya

doi:10.22159/ajpcr.2017.v10i7.18409

Authors

Vaishali Rai M Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India.
Vinitha Ramanath Pai Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India.
Samuel Kevin Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India.
Herga P Kedilaya Department of Biochemistry, Srinivas Institute of Medical Sciences and Research Centre, Mangalore, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i7.18409

Keywords:

Erythrina variegata, Cytotoxicity, Apoptosis, MDA-MB-231, Podocarpatriene

Abstract

Objective: Species of Erythrina variegata L. is reported to be used in the treatment of cancer in traditional/folklore medicine which could be explored for their anticancer potential. We aimed to evaluate the anticancer activity of crude extracts of the leaves of E. variegata with two solvents; explore the mechanism of cytotoxicity with the effective extract and correlation with the phytochemicals in the extract.

Methods: The extracts with Erythrina variegata L methanol (EVM) and chloroform (EVC) as solvents were screened for cytotoxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay on MDA-MB-231 and MCF-7 cell lines. The effective extract was further evaluated on MDA-MB-231 cells by nucleoprotein content estimation, and cell morphology was studied. High resolution liquid chromatography mass spectrometry (HRLCMS) of EVM was done to find the phytochemical composition.

Results: Among the two extracts, EVM was effective at an inhibitory concentration (IC50) value of 92 Î¼g/ml and 143 Î¼g/ml on MCF-7 and MDA-MB-231 cells, respectively. At the IC50 value (143 Î¼g/ml) the nucleoprotein content of the cells was 58.2%, and the apoptotic index was calculated to be 51.8%. EVM treated group showed significant morphological changes suggestive of apoptosis. HRLCMS revealed the presence of rutin, podocarpatriene, and cepharanthine which are known to be cytotoxic.

Conclusion: This report is a contribution toward the validation of E. variegata as a potential source of anticancer agents.

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Author Biographies

Vaishali Rai M, Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India.

Research scholar, Dept. of Biochemistry

Vinitha Ramanath Pai, Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India.

Professor, Dept. of Biochemistry

Samuel Kevin, Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India.

Intern, Yenepoya Medical college

Herga P Kedilaya, Department of Biochemistry, Srinivas Institute of Medical Sciences and Research Centre, Mangalore, Karnataka, India.

Professor & HOD, Dept of Biochemistry

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