EVALUATION OF IN VIVO ANTICANCER AND IMMUNOSTIMULATORY ACTIVITY OF FLOWERS OF MIMOSA PUDICA LINN. (FABACEAE)
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i7.18514Abstract
Objective: To investigate the in vivo anticancer and immunostimulatory activity of dichloromethane (DCM) extract of flowers of Mimosa pudica and its isolated compound 11β hydroxy-3 methoxy 1,2 dehydro crinane.
Methods: The anticancer activity was performed on Ehrlich ascites carcinoma (EAC) cell line in Swiss albino mice. The activity was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological, and histopathological parameters of EAC-bearing animals. The immunostimulatory activity was performed through carbon clearance, delayed type hypersensitivity (DTH), neutrophil adhesion and humoral antibody (HA) titer methods.
Results: At the dose of 500 and 1000 mg/kg/day p.o for the extracts and 2.5 mg/kg/day p.o. for the isolated compound, significantly decrease the tumor volume (3.46±0.135 ml, 2.25±0.153 ml, and 1.84±0.012), increased the life span (59.32%, 76.39%, and 82.43%) and significantly (p<0.05) decreased tumor weight as compared with control. Hematological profiles were found to be nearly normal level in extract treated mice compared with tumor bearing control mice. The immunostimulatory activity was also found to be effective in the above dosage regimen. The results revealed that animals treated with above doses show a significant increase in the rate of carbon clearance from blood, increase in HA titer value, increase in neutrophil adhesion and significant (p<0.05) increase in mean paw edema in DTH reactions in dose-dependent manner.
Conclusion: The results demonstrated that the extract is possessing dose-dependent anticancer activity and immunostimulatory activity attributed to the presence of crinane alkaloid.
Downloads
References
Muhit A, Apurba S, Saiful MI, Muniruddin A. Cytotoxity and antimicrobial activity of the crude extract of Abutilon indicum. Int J Pharmacogn Phytochem Res 2010;2:14-20.
Chowdhury S, Saha D, Paul S. In vitro cytotoxic activities of methanolic extract of Mimosa pudica. Bull Pharm Res 2012;2 Suppl 1:42-5.
Shukla S, Mehta A, John J, Mehta P, Vyas SP, Shukla S. Immunomodulatory activities of the ethanolic extract of Caesalpinia bonducella seeds. J Ethnopharmacol 2009;125(2):252-6.
Zaveri M, Gohil P, Jain S. Immunostimulant activity of n-butanol fraction of root bark of Oroxylum indicum. Vent. J Immunotoxicol 2006;3(2):83-99.
Joseph B, George B, Mohan J. Pharmacology and traditional use of Mimosa pudica. Int J Pharm Sci Drug Res 2013;5 Suppl 2:41-4.
Parmer F, Kushawaha N, Highland H, George LB. In vitro antioxidant and anticancer activity of M. Pudica extract. Int J Pharm Res 2014;7 Suppl 11:50-2.
Ramesh B, Pratheeba M. Studies on antimicrobial and anticancer activity of Solanum trilobatium. Asian J Pharm Clin Res 2014;7 Suppl 1:213-9.
Muthukumaran P, Shanmuganathan P, Malathi C. In vitro antioxidant evaluation of Mimosa pudica. Asian J Pharm Res 2011;2 Suppl 1:44-6.
Azmi L, Singh MK, Akhtar AK. Pharmacological and biological overview on Mimosa pudica Linn. Int J Pharm Life Sci 2011;2 Suppl 1:1226-34.
Lu Y, Fan J, Zhao Y, Chen S, Zheng X, Yin Y, et al. Immunomodulatory activity of aqueous extract of Actinidia macrosperma. Asia Pac J Clin Nutr 2007;16 Suppl 1:261-5.
Yusatina SR, Subagus W, Sitarina W, Agustinus Y. In vivo immunomodulatory effect and histopathological features of mouse liver and kidney treated with neolignans isolated from red betel (Piper crocatum Ruiz and Pav) Leaf. Trop J Pharm Res 2014;13 Suppl 10:1609-14.
Sahu RK, Nahak G. Effect of Ocimum sanctum Linn. Leaf extract in Clarias batrachus Linn. Asian J Pharm Clin Res 2014;7 Suppl 3:157-63.
Jayathirtha MG, Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine 2004;11(4):361-5.
Patel P, Asdaq SM. Immunomodulatory activity of methanolic fruit extract of Aegle marmelos in experimental animals. Saudi Pharm J 2010;18(3):161-5.
Bhadoriyal SS, Mandoriya M. Immunomodulatory effect of Tricosanthes dioica Roxb. Asian Pac J Trop Biomed 2012;7:985-7.
Yang XQ, Zheng H, Ye Q, Li RY, Chen Y. Chloroquinone inhibits cell proliferation and induces apoptosis in nasopharyngeal carcinoma cell lines. Trop J Pharm Res 2015;14 Suppl 12:2187-92.
Nayak S, Mengi S. Immunostimulant activity of the extracts and bioactives of the fruits of Morinda citrifolia. Pharm Biol 2009;47 Suppl 3:248-54.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
