ASSESSMENT OF PHYTOCHEMICAL CONSTITUENTS, IN VITRO ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL OF ULVA EXTRACTS FROM VISHAKHAPATNAM COAST

Princely S; Dhanaraju Md

doi:10.22159/ajpcr.2017.v10i8.18582

Authors

Princely S Department of Pharmaceutical Biotechnology, Research Laboratory, GIET School of Pharmacy, Rajahmundry - 533 296, Andhra Pradesh, India.
Dhanaraju Md Department of Pharmaceutical Biotechnology, Research Laboratory, GIET School of Pharmacy, Rajahmundry - 533 296, Andhra Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i8.18582

Keywords:

Marine algae, Phytochemicals, Antimicrobial, Antioxidant, 1, 1-diphenyl-2-picrylhydrazyl

Abstract

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Â Objective: Antimicrobial drug resistance is the foremost problem faced worldwide with the current antibiotic therapy in treating infectious diseases. Marine algae were considered as a potential source of biologically active compounds with antibacterial, antifungal, antiviral, and anticancer activities.

Materials and Methods: In the present investigation, the purified fractions of marine algal crude extracts of different solvents such as aqueous, ethyl acetate (EtAc), and ethanol for antioxidant (1,1-diphenyl-2-picrylhydrazyl radical scavenging assay) and antimicrobial activities (agar well diffusion assay) were evaluated.

Results: The extracts of EtAc, ethanol, and water showed minimum inhibitory concentration values of 3.125, 6.25, and 12.25 Î¼g/ml, respectively, for tested bacterial pathogens. The active fractions showed very little activity against Klebsiella pneumonia and Salmonella Typhi, and no activity was observed against Pseudomonas aeruginosa. The results of our screening showed that the EtAc marine algal fractions were active against some Gram-positive, Gram-negative bacteria and Candida albicans. The phytochemical analysis of aqueous, ethanolic, and EtAc extracts of marine algae showed the presence of the various phytochemical constituents such as carbohydrates, phenols, and amino acids. The ethanolic extracts showed the highest antioxidant activity as compared to aqueous and EtAc extracts.

Conclusion: This work can be extended further to isolate, characterize, and discover more bioactive metabolites from marine algae, which can be exploited for the production of lead molecules in pharmaceuticals for the treatment of chronic diseases.

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References

AQ1

Pereira RC, Costa-Lotufo LV. Bioprospecting for bioactives from seaweeds: Potential, obstacles and alternatives. Rev Bras Farmacogn 2012;22(4):894-905.

Msuya FE. The effect of stocking density on the performance of the seaweed Ulva reticulata as a biofilter in earthen pond channels, Zanzibar, Tanzania, Western Indian ocean. J Mar Sci 2007;6(1):65-72.

Silva M, Vieira L, Almeida AP, Kijjoa A. The marine macroalgae of the genus Ulva: Chemistry, biological activities and potential applications. J Oceanogr Mar Res 2013;1(1):1-6.

Stirk WA, Schwalb AN, Light ME, MedkovÃ¡ J, Lenobel R, Strnad M, et al. Potential medicinal value of some South African seaweeds. South Afr J Bot 2003;69(4):462-8.

Christobel GJ, Lipton AP, Aishwarya MS, Sarika AR, Udayakumar A. Antibacterial activity of aqueous extract from selected macroalgae of Southwest coast of India. Seaweed Res Util 2011;33(1-2):67-75.

Chauhan J, Kasture A. Antimicrobial compounds of marine algae from Indian coast. Int J Curr Microbiol Appl Sci 2014;3(7):526-32.

Chakraborty K, Joseph D, Praveen K. Antioxidant activities and phenolic contents of three red seaweeds (division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India. J Food Sci Technol 2015;52(4):1924-35.

Radhika D, Veerabahu C, Priya R, Mohaideen A. A comparative study of bio-potential of crude and fractionated extracts of some sea weeds from Tuticorin coast. Int J Phytopharmacol 2014;5(1):27-30.

Pramitha VS, Lipton AP. Antimicrobial effect of Ulva fasciata delile, 1813 solvent extracts against multidrug resistant human pathogenic bacteria and fish pathogens. Indian J Mar Sci 2014;43(9):1-9.

Moomeen HS, Padmavathy S. In vitro antagonistic activity of marine seaweed extracts against human pathogens. Unique J Pharm Biol Sci 2014;2(4):7-13.

Karthick P, Mohanraju R, Murthy KN, Ramesh CH. Antibacterial activity of seaweeds collected from South Andaman, India. J Algal Biomass Util 2015;6(1):33-6.

Desikachary TV. Cyanophyta. ICAR Monographs on Algae. New Delhi: ICAR; 1959. p. 686.

Anand NL, Radha RS, Hopper S, Ravati G, Subramianan TD. Perspectives in Phycology. New Delhi: Today and Tomorrowâ€™s Printers and Publishers; 1990. p. 383-91.

Prescott GW. Algae of the Western Great Lake Areas. Dubuque, Iowa: W.M.C. Brown Company Publisher; 1962.

Kavanagh F. Analytical Microbiology. Vol. II. New York, London: Academic Press; 1992. p. 241-3.

Bano A, Ayub Z. Antibacterial and antifungal activity in three species of Siphonaria (Gastropoda: Pulmonata) collected from rocky ledge of Mubarak village, Karachi. Pak J Zool 2012;44(6):1493-7.

Devi JA, Balan GS, Periyanayagam K. Pharmacognostical study and phytochemical evaluation of brown seaweed Sargassum wightii. J Coast Life Med 2013;1(3):199-204.

Seenivasan R, Rekha M, Indu H, Geetha S. Antibacterial activity and phytochemical analysis of selected seaweeds from Mandapam Coast, India. J Appl Pharm Sci 2012;2(10):159-69.

Abdel-Hamid MI, Belal SA, Azab YA, Abdel-Mogib M, Abdel-Aal EI. Nutritional value of some selected green microalgae. J Environ Sci 2015;44(3):455-67.

Ismail GA. Biochemical composition of some Egyptian seaweeds with potent nutritive and antioxidant properties. Food Sci Technol 2016;65:1-9.

Chandru S, Hephzibah AJ, Rajesh P, Kannan VR. Screening, evaluation of vibriocidal activity and characterization of bioactive substance from marine cyanobacteria. Afr J Microbiol Res 2013;7(17):1681-7.

Wagner H, Bladt S. Plant Drug Analysis, a Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer; 2009. p. 306-64.

Widowati I, Lubac D, Puspita M, Bourgougnon N. Antibacterial and antioxidant properties of the red alga Gracilaria verrucosa from the North coast of Java, Semarang, Indonesia. Int J Latest Res Sci Technol 2014;3(3):179-85.

Oumaskour K, Boujaber N, Etahiri S, Assobhei O. Anti-inflammatory and antimicrobial activities of twenty-three marine red algae from the coast of Sidi Bouzid (El Jadida-Morocco). Int J Pharm Pharm Sci 2013;5(3):145-9.

Mhadhebi L, Chaieb K, Bouraoui A. Evaluation of antimicrobial activity of organic fractions of six marine algae from Tunisian Mediterranean coasts. Int J Pharm Pharm Sci 2012;4(1):534-7.

Mole MN, Sabale AB. Antibacterial activity of green seaweeds Chaetomorpha and Enteromorpha. Int J Pharm Bio Sci 2013;4(3):716 20.

Omar HH, Shiekh HM, Gumgumjee NM, El-Kazan MM, El Gendy AM. Antibacterial activity of extracts of marine algae from the red sea of Jeddah, Saudi Arabia. Afr J Biotechnol 2012;11(71):13576-85.

Etcherla M, Rao GM. In vitro study of antimicrobial activity in marine algae Caulerpa taxifolia and Caulerpa racemosa (C. Agardh). Int J Appl Biol Pharm Technol 2014;5(2):57-62.

Srivastava N, Saurav K, Mohanasrinivasan V, Kannabiran K, Singh M. Antibacterial potential of macroalgae collected from Madappam coast, India. Br J Pharmacol Toxicol 2010;1(2):72-6.

Akgul R, Suerdem TB, Akgul F. Antimicrobial activities of some marine algae and some Cyanobacteria from Canakkale (Turkey). J Algal Biomass Util 2013;4(3):35-40.

Kolsi RB, Frikha D, Jribi I, Hamza A, Feki L, Belghith K. Screening of antibacterial and antifongical activity in marine macroalgae and magnoliophytea from the coast of Tunisia. Int J Pharm Pharm Sci 2015;7(3):47-51.

Saranya C, Parthiban C, Anantharaman P. Evaluation of antibacterial and antioxidant activities of seaweeds from Pondicherry coast. Adv Appl Sci Res 2014;5(4):82-90.

Sudha K, Priya KM, Kumari NV, Palanichamy V. Screening of antioxidant potential of green alga Codium adhaerens. Int J Drug Dev Res 2014;6(4):103-11.

Rhimou B, Hassane R, Nathalie B. Antioxidant activity of Rhodophyceae extracts from Atlantic and Mediterranean Coasts of Morocco. Afr J Plant Sci 2013;7(3):110-17.

Yildiz G, Dere E, Dere S. Comparison of the antioxidative components of some marine macroalgae from Turkey. Pak J Bot 2014;46(2):753-7.

Gouda S, Moharana RR, Das G, Patra JK. Free radical scavenging

potential of extracts of Gracilaria verrucosa (L) (Harvey): An economically important seaweed from Chilika Lake, India. Int J Pharm Pharm Sci 2013;6(1):707-10.

SimiÄ‡ S, KosaniÄ‡ M, RankoviÄ‡ B. Evaluation of in vitro antioxidant and antimicrobial activities of green microalgae Trentepohlia umbrina. Notulae Bot Horti Agrobot 2012;40(2):86-91.