• THIRUNAVUKKARASU P Department of Biotechnology, Dr. M.G.R. Educational and Research Institute University, Chennai - 600 095, Tamil Nadu, India.
  • Asha S Department of Biochemistry, DKM College for Women, Vellore, Tamil Nadu, India.
  • Rohith Kumarreddy Department of Biotechnology, Dr. M.G.R. Educational and Research Institute University, Chennai - 600 095, Tamil Nadu, India.
  • Ramanathan T Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Cuddalore - 608 502, Tamil Nadu, India
  • Sudhakar N Department of Biotechnology, Dr. M.G.R. Educational and Research Institute University, Chennai - 600 095, Tamil Nadu, India.



Helicobacter pylori, Gastric patient, Biopsy, Gram


Objective: This study was to isolate and identify the Helicobacter pylori from the biopsy samples of the gastric patient.

Methods: Gastric biopsies were collected from the antral region of the gastric patient. Out of 96 patients, 59 males and 37 females in the age group between 11 and 70 years old were selected. A serial dilution of the sample was made. The bacterial colonies were examined on the basis of Gram staining, colony morphology, and biochemical reactions such as catalase, urease, oxidase, nitrate reduction, glycine utilization, growth (different media, different temperature, and salt tolerance), and antibiotic sensitivity

Result: From the findings, it was found that 75% (65% of male and 35% of female) have H. pylori infection remaining 25% were not infected. The rate of infection was found to be more in age group 55-65 and less in age group below 25. Among 75% of H. pylori infected patients, 72% are affected with ulcer, 19% with gastric cancer, and 8.3% found to be non-gastric inflammated. Gram staining result declared that the isolated bacteria from the biopsy sample observed to be Gram-negative, spiral shaped rod. Biochemical reports produced positive indication to all the tests.

Conclusion: Based on the morphological, staining and biochemical test result, it was confirmed that the isolated bacteria was found to be H. pylori.Gastric cancer is the fourth most common cancer and the second leading cause of cancer-related to death worldwide. In 1994, the international agencyfor research on cancer classified H. pylori as a Class I (definite) carcinogen, as H. pylori infection is considered as an important trigger in the processof carcinogenesis of both types of distal gastric cancer.



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Author Biography

THIRUNAVUKKARASU P, Department of Biotechnology, Dr. M.G.R. Educational and Research Institute University, Chennai - 600 095, Tamil Nadu, India.



Ashwini P, Sumana MN, Shilpa U, Mamatha P, Dhananjaya BL, Farhan Z, et al. A review on Helicobacter pylori: Its biology, complications and management. Int J Pharm Pharm Sci 2014;7(1):14-20.

Farinha P, Gascoyne RD. Helicobacter pylori and MALT lymphoma.Gastroenterology 2005;128(6):1579-605.

Solnick JV, O’Rourk JL, Vandamme P, Lee A. The genus Helicobacter. In: Prokaryotes. 3rd ed., Vol. 7. New York: Springer-Verlag; 2006.

Czinn S. Helicobacter pylori infection: Detection, investigation, and management. J Pediatr 2005;146(3):21-6.

Rothenbacker D, Brenner H. Burden of Helicobacter pylori and H.pylori-related diseases in developed countries: Recent developments

and further implications. Microbes Infect 2003;5(8):693-703.

Warren JR, Marshall BJ. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet1983;1(8390):1311-5.

Goodwin CS. Helicobacter pylori gastritis, peptic ulcer, and gastric cancer: Clinical and molecular aspects. Clin Infect Dis 1997;25:1017-9.

Coghlan JG, Gilligan D, Humphries H, McKenna D, Dooley C, Sweeney E, et al. Campylobacter pylori and recurrence of duodenal ulcers a 12-months follow-up study. Lancet 1987;2:1109-11.

Rauws EA, Tytgat GN. Cure of duodenal ulcer associated with eradication of Helicobacter pylori. Lancet 1990;335(8705):1233-5.

Graham DY, Klein PD, Evans DJ. Campylobacter pylori detected noninvasively by the 13C-urea breath test. Lancet 1987;1(8543):1174-7.

Mobley HL, Mendz GL, Hazell SL, editors. Overview. In: Helicobacter pylori: Physiology and Genetics. Washington, DC: ASM Press; 2001.p. 3-5.

Marshall BJ. The Discovery that Helicobacter pylori, a spiral bacterium, caused peptic ulcer disease. In: Marshall BM, editor. Helicobacter Pioneers. Singapore: Blackwell Science Asia; 2002. p. 165-202.

Rossi G, Rossi M, Vitali CG, Fortuna D, Burroni D, Pancotto L, et al. A conventional beagle dog model for acute and chronic infection with Helicobacter pylori. Infect Immunol 1999;67:3112-20.

On SL. Identification methods for Campylobacters, Helicobacters and related organisms. Clin Microbiol Rev 1996;9(3):405-22.

Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. Clin Microbiol Rev 1997;10(4):720-41.

de Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, et al., editors. The firmicutes. Bergey’s Manual of Systematic Bacteriology. 2nd ed., Vol. 3. New York: Springer; 2006.

Christensen WB. Urea Decomposition as a means of differentiating proteus and paracolon cultures from each other and from Salmonella

and Shigella types. J Bacteriol 1946;52:461-6.

Smibert RM, Krieg NR. General characterization. In: Gerdhardt P, Murray RG, Costilow RN, editors. Manual of Methods for General

Bacteriology. Washington, DC: American Society for Microbiology; 1981. p. 409-43.

Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956;178(4535):703.

Stanier RY, Palleroni NJ, Doudoroff M. The aerobic Pseudomonads: A taxonomic study. J Gen Microbiol 1966;43:159.

Parsonnet J, Shmuely H, Haggerty T. Fecal and oral shedding of Helicobacter pylori from healthy infected adults. J Am Med Assoc


Dore AG, Lundin ER, Jensen LN, Birkeland O, Elliasen PE, Fichler C.Principle tectonic events in the evolution of the Northwest European Atlantic margin. In: Fleet AJ, Boldy SA, editors. Petroleum Geology of Northwest Europe: Proceedings of the 5th Conference. London: Geological Society; 1999.

Rowland M. Transmission of Helicobacter pylori: Is it all child’s play? Lancet 2000;355:332-3.

Sasaki K, Tajiri Y, Sata M, Fujii Y, Matsubara F, Zhao M, et al. Helicobacter pylori in the natural environment. Scand J Infect Dis


Plastridge WN. Bovine mastitis: A review. J Dairy Sci 1958;41:1141-81.

Fujimura S, Kawamura T, Kato S, Tateno H, Watanabe A. Detection of Helicobacter pylori in cow’s milk. Lett Appl Microbiol 2002;35(6):504-7.

Al-Sulami A, Al-Kiat HS, Bakker LK, Hunoon H. Primary isolation and detection of Helicobacter pylori from dyspeptic patients: A simple, rapid method. East Mediterr Health J 2008;14(2):268-76.

Al-Ajeel AM. Helicobacter pylori in common upper gastrointestinal disorders in Al-Anbar governorate. Al Anbar Univ J 1996;1:10-4.

Fatah HT, Al-Janabi AH. Prevalence of Helicobacter pylori in patients with peptic ulcer in Najaf. Al Kufa Univ J 1998;2:88-92.

Young KA, Akyon Y, Rampton DS, Barton SG, Allaker RP, Hardie JM, et al. Quantitativeculture of Helicobacter pylori from gastric juice: The potential for transmission. J Med Microbiol 2000;49:343-7.

Milyani RM, Barhameen AA. Survival of Helicobacter pylori in tap water and apple juice. J Med Res Inst 2003;24(3):158-68.

Karari EM. Endoscopic finding and the prevalence of Helicobacter pylori in chronic renal failure patients. East Afr Med J 2000;77(8):406-9.

Hartzen SH, Andersen LP, Bremmelgaard A, Colding H, Arpi M. Antimicrobial susceptibility of testing of 230 Helicobacter pylori

strains: Importance of medium, inoculums and incubation time. Antimicrob Agents Chemother 1997;41:2634-9.

Piccolomini R, Di Bonaventura G, Festi D, Catamo G, Laterza F, Neri M. Optimal combination of media for primary isolation of

Helicobacter pylori from gastric biopsy specimens. J Clin Microbiol 1997;35(6):1541-4.

Kumar AR, Venkateswaramurthy N, Vijayabaskaran M, Perumal P. Formulation of clarithromycin loaded mucoadhesive microspheres

by emulsification - Internal gelation technique for anti – Helicobacter pylori therapy. Int J Pharm Pharm Sci 2010;3(2):173-7.

Correa P. Helicobacter pylori and gastric carcinogenesis. Am J Surg Pathol 1995;19:37-43.

Ekstrom AD, Meltzer J, McNaughton BL, Barnes CA. NMDA receptor antagonism blocks experience-dependent expansion of hippocampal ‘place fields’. Neuron 2001;31(4):631-8.

Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M, et al. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 2001;345(11):784-9.

Blecker U, McKeithan TW, Hart J, Kirschner BS. Resolution of Helicobacter pylori - Associated gastric lymphoproliferative disease in

a child. Gastroenterology 1999;109(3):973-7.

Rodrigues MN, Queiroz DM, Bezerra Filho JG, Pontes LK, Rodrigues RT, Braga LL. Prevalence of Helicobacter pylori infection in

children from an urban community in North-East Brazil and risk factors for infection. Eur J Gastroenterol Hepatol 2004;16(2):201-5.



How to Cite

THIRUNAVUKKARASU P, A. S, R. Kumarreddy, R. T, and S. N. “SURVEY, ISOLATION, BIOCHEMICAL CHARACTERIZATION, AND IDENTIFICATION OF HELICOBACTER PYLORI FROM GASTRIC PATIENT BIOPSY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 6, June 2017, pp. 411-6, doi:10.22159/ajpcr.2017.v10i6.18670.



Original Article(s)