ALLIUM SATIVUM LINN. CONTAINS LINEAR ALKYLBENZENE SULFONATES THAT ALTER MEMBRANE FLUIDITY FOR THE INHIBITION OF MYCOBACTERIUM TUBERCULOSIS H37RA

Rajiniraja Muniyan; Sabareesh Varatharajan; Saba Naz; Vinay K Nandicoori; Jayaraman Gurunathan

doi:10.22159/ajpcr.2017.v10i9.18693

Authors

Rajiniraja Muniyan Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India
Sabareesh Varatharajan Centre for Bio-Separation Technology, VIT University, Vellore - 632 014, Tamil Nadu, India
Saba Naz National Institute of Immunology, Aruna Asaf Ali Marg,New Delhi - 110 067, India
Vinay K Nandicoori National Institute of Immunology, Aruna Asaf Ali Marg,New Delhi - 110 067, India
Jayaraman Gurunathan Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.18693

Keywords:

Bioactivity-guided fractionation, Microplate alamar blue assay, Liquid chromatography-mass spectrometry, Cell leakage assay, Scanning electron microscope

Abstract

Â

Â Objectives: The purpose of the study is to characterize antimycobacterial phytoconstituent from ethyl acetate extract of dried bulbs of Allium sativum Linn. (Alliaceae) and elucidating the probable mode of action of the bioactive molecule.

Methods: Serial extraction, Mycobacterium tuberculosis assay by agar well diffusion method, minimal inhibitory concentration by microplate alamar blue assay, Fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectroscopy, liquid chromatography (LC)-electrospray ionization (ESI)-mass spectrometry (MS)/MS, cell leakage assay, scanning electron microscopy (SEM), inhibition property of linear alkylbenzene sulfonate (LAS) in the presence of rifampicin on M. tuberculosis were performed.

Results: Ethyl acetate extract displayed significant inhibition properties against M. tuberculosis H37Ra (MTCC 300). Subsequently, the bioactivity-guided fractionation was employed to purify the phytochemical. Analysis of FT-IR, LC-MS (ESI), 1H, and13C-NMR spectrum revealed that the bioactive phytochemicals are the variants of LAS, with C12-alkyl being predominant, and the minimum inhibitory concentration was found to be 5.56 Î¼g/ml. Morphological examination by SEM and cell leakage assay indicated that these molecules change the membrane fluidity.

Conclusion: The results thus suggest the possibility of using low concentrations of LAS to effect changes in membrane fluidity, thereby enhancing the efficacy of antibiotic treatment.

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Author Biography

Rajiniraja Muniyan, Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India

Department of Biotechnology

School of Bio-Sciences and Technology

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