INHIBITION OF ADVANCED GLYCATION END-PRODUCT FORMATION BY QUERCETIN AND CATECHIN: AN ALTERNATIVE THERAPY FOR TREATING DIABETIC COMPLICATIONS

Angeline Julius; Waheeta Hopper

doi:10.22159/ajpcr.2017.v10i11.19412

Authors

Angeline Julius Department of Biotechnology, School of Bioengineering, Faculty of Engineering and Technology, SRM University, Chennai, Tamil Nadu, India.
Waheeta Hopper Department of Biotechnology, School of Bioengineering, Faculty of Engineering and Technology, SRM University, Chennai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.19412

Keywords:

Aldose reductase inhibitors, Advanced glycation end-products, High-throughput virtual screening, Natural compounds, Diabetic complications

Abstract

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Â Objective: The objective of this research was to determine early advanced glycation end-product (AGE) inhibition by natural aldose reductase inhibitors (ARIs), quercetin and catechin.

Methods: The assay mixture (4 ml) consisted of 2 ml of 50 mM phosphate-buffered saline (pH 7.4), 50 Î¼g/Î¼l bovine serum albumin (BSA), and 2 mM glucose with or without the inhibitor. The test samples were treated with three different concentrations (10 mM, 20 mM, and 40 mM) of quercetin and catechin. High-throughput screening-based assay was adapted to perform the BSA-glucose test to determine the induction of AGE formation and its inhibition by quercetin, and catechin, using the fluorescence of the AGE-BSA sample at excitation and emission wavelengths of 350 and 450 nm.

Result: The ARIs, quercetin and catechin inhibited early glycation with an inhibitory concentration value of 15.58 mM and 35.01 mM, respectively.

Conclusion: The suppression of AGEs formation by natural inhibitors of aldose reductase would provide an alternative approach to the control of diabetic complications.

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Author Biographies

Angeline Julius, Department of Biotechnology, School of Bioengineering, Faculty of Engineering and Technology, SRM University, Chennai, Tamil Nadu, India.

Research scholar, Department of Biotechnology

Waheeta Hopper, Department of Biotechnology, School of Bioengineering, Faculty of Engineering and Technology, SRM University, Chennai, Tamil Nadu, India.

Associate professor, Department of Biotechnology

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