THE EXISTENCE OF GENE DNA POLYMERASE MUTATION FROM POSITIVE HEPATITIS B SAMPLES IN BANDUNG, INDONESIA

Tina Rostinawati Rostinawati; Debbie Sofie Retnoningrum; Desi Pratiwi

doi:10.22159/ajpcr.2017.v10s2.19493

Authors

Tina Rostinawati Rostinawati Padjadjaran University
Debbie Sofie Retnoningrum
Desi Pratiwi

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s2.19493

Keywords:

Hepatitis B virus, Mutation, Gene encoding for reverse transcriptase deoxyribonucleic acid polymerase

Abstract

Objective: To complete mutation existence in reverse transcriptase domain of the viral Polymerase.

Methods: The study was done by amplification step of viral polymerase gene fragment, agarose gel electrophoresis, PCR product purification with GFX column kit, sequencing and sequencing result analysis. The samples were derived from Clinical Laboratory in Bandung Indonesia.

Results: The result showed mutations in DNA fragment encoding for RT domain viral polymerase in sample 6, 7 and 8. There were mutations leading to amino acid substitution L526S in sample 6, D551E in sample 7 and D552E in sample 8.

Conclusion: D551E and D552E substitution occurred in YMDD motif RT DNA polymerase that produced YMDE mutant. L526S, D551E and D552E were estimated as antivirus-resistance mutants that have never been reported before.

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Author Biography

Tina Rostinawati Rostinawati, Padjadjaran University

Biology Pharmacy Departement

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