IN VIVO TEST OF ANTIMALARIAL ACTIVITY FROM DICHLOROMETHANE-ETHYL ACETATE-METHANOL FRACTIONS OF MUNDU'S BARK(GARCINIA DULCIS (ROXB.) IN SWISS WEBSTER MICE
DOI:
https://doi.org/10.22159/ajpcr.2017.v10s2.19513Abstract
Objective:Genus Garcinia is well-known having rich xanthone compound contents and several of those are having biological activity as antimalarial. The aims of this research wereto determine in vivoantiplasmodialactivityof dichloromethane-ethyl acetate-methanol fractions of Mundu's bark (Garciniadulcis (Roxb.) Kurz) especially fraction Vagainst Plasmodium bergheiand to determineeffective dose.
Methods: Making fraction V from ethyl acetate extractsof Mundu's bark with vacuum column chromatographymethod was applied using dichloromethane-ethyl acetate-methanol. The product was monitored by thin layer chromatography using silica gel GF-254 and a mobile phase of chloroform: ethyl acetate (6:4).The same profiles from eluent composed fraction 18 and 19 were categorized as fraction V.Those were tested in each of groups animals with a doseof 12.5; 25; 50 and 100 mg/kg body weight. Antimalarial activityassessments have been performed with the in vivoPlasmodium bergheitest. Plasmodial activity was obtained by calculating percentage of parasitaemia, parasitaemia inhibition and ED50 determination. ED50value determined based on the relationship between dose and the percentage of parasite growth inhibition by probit analysis.
Results: In this research, Antiplasmodial activity of fraction V from Mundu's bark displayed in dose of 50 mg/kg bw and 100 mg/kg bw with inhibition parasite growth by values of 47.255% and 12.761%, respectively.
Conclusion: According to the research, fraction V of Mundu's bark (Garcinia dulcis (Roxb.) Kurz) had the most potential antiplasmodial activity by in vivo test for male Swiss-Webster mice. The value of ED50 was reached by 47.424 mg/kg bw.
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Keywords: Mundu's bark, Antiplasmodial activity, ED50.
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Lannang AM, Komguem, J., Ngninzeko, FN., Tangmouo, JG., Lontsi, D., Ajaz, A., Choudhary, MI., Ranjit, R., Devkota, KP., Sodengam, BI., . Bangangxanthone A and B two xanthones from the stem bark of GarciniapolianthaOliv. Phytochemistry2005;66: 2351-5.
Wernsdorder W.H., and D. Payne. The Dinamiesof Drug resistence in Plasmodium falciparum. Pharmacol. Ther. 1991; 50: 95-121.
Wernsdorfer, W.H..Epidemology of Drug Resistence in Malaria. Acta. Trop 1994;56:143-56.
Vroman J.A., M.A. Gaston, M.A. Avery. Curr, Pharm. Design, 5 (1999) 101- 38.
Merza, J., Aumond, M.C., Rondeau, D., Dumontet, V., Ray., A.M.L., and Seraphin, D., dkk., ,PrenylatedXanthones and Tocotrienols from Garciniavirgata.Phytochemistry2004;65: 2915- 20
Syamsudin, Tjokrosonto, S., Wahyuono, S., danMustofa, Aktivitasantiplasmodiumdariduafraksiekstrak n-heksanakulitbatangasamkandis (Garcinia parvifoliaMiq).MajalahFarmasi Indonesia 2007; 18 Suppl 4: 210-5.
Likhtiwitayawuid K, Chanmahasathien W, Ruangrungsi N, Krungkrai J. Xanthones with antimalarial activity from Garcinia dulcis. Planta Med 1998;64Suppl 3: 281–2.
Sukamat and Ersam, T, DuaSenyawaSanton Dari KayuBatangMundu Garcinia Dulcis (Roxb.) Kurz. SebagaiAntioksidan. Proceeding of National Seminar on Chemistry VIII, Surabaya, 8 Agustus 2006 .p.1-4
Peters, W., Chemotherapy and drugs resistance in malaria. New York: Academic Press, Inc.; 1987. Suppl 1. p. 145273
Widodo GP, Rahayu MP..AktivitasAntimalariaEkstrakEtilAsetatKulitBatangMundu (Garcinia dulcisKurz.). MajalahFarmasi Indonesia 2010; 21 Suppl 4: 241.
Hesturini RJ, Widodo GP, Rahayu MP..Efek Anti Plasmodium Ekstrak n-heksanaKulitBatangMundu (GarciniadulcisKurz.) padaMencitJantan Swiss Webster yang Diinduksi Plasmodium berghei. JurnalFarmasi Indonesia 2011; 8 Suppl 1: 32-4.
Munoz V, Sauvain M, Bourdy G, Callapa J, Bergeron S, Rojas I, Bravo JA, Balderrama L, Ortiz B, Gimenez A, Deharo E. A search for natural bioactive compounds in Bolivia through a multidisciplinary approach: Part I. Evaluation of the antimalarial activity of plants used by the Chacobo Indians. J Ethnopharmacol 2000;69 Suppl 2: 127-37
Riscoe M, Kelly JX, Winter R. Xanthones as anti-malaria: discovery, mode of action and optimization. Curr. Med. Chem 2005;12:2539-49
Ignatushchenko, M.V., Winter, R.W., Bachinger, H.P., Hinrichs, D.J., and Riscoe, M.K., Xanthones as antimalarial agents, studies of a possible mode of action.FEBS Letter1997; 409:67-73.
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