EVALUATION OF A RAPID TEST TO DETECT CARBAPENEMASE PRODUCTION AMONG GRAM-NEGATIVE BACILLI.

Vishnu Nagalapuram; Jayalakshmi J

doi:10.22159/ajpcr.2018.v11i1.19605

Authors

Vishnu Nagalapuram Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
Jayalakshmi J Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i1.19605

Keywords:

Carba NP test, Carbapenemase producer, Modified Hodge test

Abstract

Objective: There is a need for an early detection of carbapenemase-producing Gram-negative bacilli to reduce the morbidity and mortality associated with it. The objective of this study is to evaluate the usefulness of the Carba NP test in detecting carbapenemase-producing Gram-negative bacilli.

Methods: This prospective experimental study was conducted in 2013 at PSG Institute of Medical Sciences and Research, Coimbatore. In this study, 120 consecutive Gram-negative bacilli clinical isolates identified as carbapenem resistant as per the Clinical and Laboratory Standard Institute guidelines were subjected to Carba NP.

Results: About 62.5% of the carbapenem-resistant isolates were found to be carbapenemase producers by modified Hodge test. The sensitivity of the Carba NP test was found to be 92% and the specificity was 100%. The most common bacterial isolate was Acinetobacter baumannii, and the most common sample was urine.

Conclusion: The Carba NP test is a simple inexpensive, rapid, reproducible, sensitive test that can be performed directly from the colonies. It improves early detection of patients infected or colonized with carbapenemase in the hospital.

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Author Biography

Jayalakshmi J, Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.

Professor, Department of Microbiology

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