MICROBICIDAL EVALUATION OF PLANT EXTRACTS - IRON METAL IONS COMPLEX
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i10.19687Keywords:
Mint, Methi, Neem, Antimicrobial activity, Bacillus, Aspergillus, Rhizopus, Iron metal ionsAbstract
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 Objective: The aim of this study is to screen and evaluate the antimicrobial or microbicidal effect of plant metabolites, conjugated with essential iron metal ions.
Methods: First batch of study includes screening of antimicrobial activity of leaves of mint (Mentha), methi (Trigonella foenum-graecum), and neem (Azadirachta indica) against Bacillus, Aspergillus, and Rhizopus sp., respectively. Inhibition of microbial growth and propagation was investigated using agar well diffusion method (minimum inhibitory concentration evaluation). Antibiotic agent, streptomycin sulfate, and antimycotic agent, and fluconazole was used as a positive control. The polar, non-polar, and aqueous extracts of plants leaves of abovementioned plant species were screened for their antibacterial activity against Bacillus sp. and antifungal activity against Aspergillus and Rhizopus sp. and their mix culture, respectively. Second batch of study includes evaluation of combinatorial effect of plant extracts of mint (Mentha piperita), methi (T. foenum-graecum), and neem (A. indica) complexed with ferric ions (Fe3+, Iron metal ions) in concentrations at acceptable dosage range.
Results: The antibacterial and antifungal activity of the plants leaves extracts in different combinations was evaluated. The plant extracts from selected plant of neem, methi, and mint after conjugation with essential iron metal ions and at a concentration of 100 mg/ml show an enhanced microbicidal (2-3 fold) activity compared to only plant extracts used in the experiment.
Conclusion: Based on the above investigation, it can be concluded that the plant extracts possessing antimicrobial property when conjugated with essential metal ions (iron metal ions) show enhanced antimicrobial efficacy compared to only plant extracts used. The result from this investigation could be very helpful in the food preservation and packaging industry.
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Maragathavalli S, Brindha S, Kaviyarasi NS, Annadurai B, Gangwar SK. Antimicrobial activity in leaf extract of neem (Azadirachta indica Linn.). Int J Sci Nat 2012;3(1):110-3.
Kumbalwar MM, Ingle AB, Shende MH. Antimicrobial activity of Mentha arvensis L (Pudina) against on Gram-negative bacteria. Microbiology 2014;4(4):154.
Sun HQ, Lu XM, Gao PJ. The exploration of the antibacterial mechanism of FE(3+) against bacteria. Braz J Microbiol 2011;42:410-4.
Legan JD. Mould spoilage of bread: The problem and some solutions. Int Biodeterior Biodegradation 1993;32(1-3):33-53.
Coda R, Rizzello CG, Nigro F, De Angelis M, Arnault P, Gobbetti M. Long-term fungal inhibitory activity of water-soluble extracts of Phaseolus vulgaris cv. Pinto and sourdough lactic acid bacteria during bread storage. Appl Environ Microbiol 2008;74(23):7391-8.
Denning DW, O’Driscoll BR, Hogaboam CM, Bowyer P, Niven RM. The link between fungi and severe asthma: A summary of the evidence. Eur Respir J 2006;27(3):615-26.
Arcella D, Le Donne C, Piccinelli R, Leclercq C. Dietary estimated intake of intense sweeteners by Italian teenagers. Present levels and projections derived from the INRAN-RM-2001 food survey. Food Chem Toxicol 2004;42(4):677-85.
Gujar JK, Talwankar D. Efficacy of some botanicals against seeding rot of oil yielding plants. Multilogic Sci 2012;2(2):122-5.
Sievert D, Hoseney RC. Bread and other baked products. Ullmann’s Encyclopedia of Industrial Chemistry. Weinheim, Germany: Wiley-VCH; 2007.
Hunt L, Robbins L. Food expenditure patterns of Canadian consumers. Food Mark Comment 2009;11:42-51.
Ibrahim MB. Anti-microbial effects of extract leaf, stem and root bark of Anogeissus leiocarpus on Staphylococcus aureaus, Streptococcus pyogenes, Escherichia coli and Proteus vulgaris. J Pharm Dev 1997;2:20-30.
Vinoth B, Manivasagaperumal R, Rajaravindran M. Phytochemical analysis and antibacterial activity of Azadirachta indica A. Juss. Int J Res Plant Sci 2012;2(3):50-5. 13. Kumar PS, Mishra D, Ghosh G, Panda CS. Biological action and medicinal properties of various constituent of Azadirachta indica (Meliaceae): An overview. Ann Biol Res 2010;1(3):24-34.
Mann A, Banso A, Clifford LC. An antifungal property of crude plant extracts from Anogeissus leiocarpus and Terminalia avicennioides. Tanzan J Health Res 2008;10(1):34-8.
Marchand E, Verellen-Dumoulin C, Mairesse M, Delaunois L, Brancaleone P, Rahier JF, et al. Frequency of cystic fibrosis transmembrane conductance regulator gene mutations and 5T allele in patients with allergic bronchopulmonary aspergillosis. Chest 2001;119(3):762-7.
Mondall NK, Mojumdar A, Chatterje SK, Banerjee A, Datta JK, Gupta S. Antifungal activities and chemical characterization of Neem leaf extracts on the growth of some selected fungal species in vitro culture medium. J Appl Sci Environ Manage 2009;13(1):49-53.
Niku-Paavola ML, Laitila A, Mattila-Sandholm T, Haikara A. New types of antimicrobial compounds produced by Lactobacillus plantarum. J Appl Microbiol 1999;86(1):29-35.
O’Driscoll BR, Hopkinson LC, Denning DW. Mold sensitization is common amongst patients with severe asthma requiring multiple hospital admissions. BMC Pulm Med 2005;5(1):4.
Okkers DJ, Dicks LM, Silvester M, Joubert JJ, Odendaal HJ. Characterization of pentocin TV35b, a bacteriocin-like peptide isolated from Lactobacillus pentosus with a fungistatic effect on Candida albicans. J Appl Microbiol 1999;87(5):726-34.
Pattison TL, Lindsay D, Von Holy A. Natural antimicrobials as potential replacements for calcium propionate in bread. S Afr J Sci 2004;100(7-8):342-8.
Barnes PD, Marr KA. Aspergillosis: Spectrum of disease, diagnosis, and treatment. Infect Dis Clin North Am 2006;20(3):545-61, vi.
Rementeria A, López-Molina N, Ludwig A, Vivanco AB, Bikandi J, Pontón J, et al. Genes and molecules involved in Aspergillus fumigatus virulence. Rev Iberoam Micol 2005;22(1):1-23.
Rizzello CG, Cassone A, Coda R, Gobbetti M. Antifungal activity of sourdough fermented wheat germ used as an ingredient for bread making. Food Chem 2011;127(3):952-9.
Rosenkvist H, Hansen A. Contamination profiles and characterisation of Bacillus species in wheat bread and raw materials for bread production. Int J Food Microbiol 1995;26(3):353-63.
Saranraj P. Microbial spoilage of bakery products and its control by preservatives. Int J Pharm Biol Arch 2012;3(1):38-48.
Schubert MS. Allergic fungal sinusitis: Pathogenesis and management strategies. Drugs 2004;64(4):363-74.
Seiler D. Preservation of bakery products. In: Proceedings of the Institute of Food Science and Technology. Vol. 17; 1984. p. 31-9.
Cheesbrough M. District Laboratory Practice in Tropical Countries. Cambridge (UK): Cambridge University Press; 2006.
Sofos JN, Busta FF. Antimicrobial activity of sorbate. J Food Prot 1981;44(8):614-22.
Stevens DA, Moss RB, Kurup VP, Knutsen AP, Greenberger P, Judson MA, et al. Allergic bronchopulmonary aspergillosis in cystic fibrosis - State of the art: Cystic Fibrosis Foundation Consensus Conference. Clin Infect Dis 2003;37 Suppl 3:S225-64.
Tillie-Leblond I, Tonnel AB. Allergic bronchopulmonary aspergillosis. Allergy 2005;60(8):1004-13.
Voysey PA, Hammond JC. Reduced-additive breadmaking technology. In: Technology of Reduced-Additive Foods. US: Springer; 1993. p. 80-94.
Sharma Y, Bhardwaj M, Nagar A, Bhagat N. Isolation and characterization of MDR bacteria from in-vitro culture of Bacopa monnieri and supplementation of natural extracts to control bacterial contamination. Int J Pharm Pharm Sci 2013;8(11):102-7.
Sharma Y, Nagar A, Shukla S. Antibacterial activity and phytochemical screening of Adenium obesum (Desert rose) leaf. Int J Pharm Bio Sci 2015;6(3):85-92.
Gupta Y, Sharma D, Sharma Y. Bactericidal activity of different parts of Azadirachta indica on probiotic microbes. Asian J Pharm Clin Res 2016;9:50-3.
Bounds G. Death by mint oil: Natural pesticides. Wall St J 2009.
Mattazi N, Farah A, Fadil M, Chraibi M, Benbrahim KF. Essential oils analysis and antibacterial activity of the leaves of Rosmarinus officinalis, Salvia officinalis and Mentha piperita cultivated in Agadir (Morocco). Int J Pharm Pharm Sci 2015;7(9):73-9.
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