• Patil Sunita Department of Microbiology, School of Life Sciences, Karpagam University, Coimbatore - 641 021, Tamil Nadu, India.
  • Muthusamy Palaniswamy Department of Microbiology, School of Life Sciences, Karpagam University, Coimbatore - 641 021, Tamil Nadu, India.



Silver nanoparticles, Aegle marmelos, Size, Bacterial skin pathogens, Antibacterial activity



 Objective: Bacterial skin infection is one of the most common causes of childhood morbidity in India. Mostly, it is caused by Streptococcus and Staphylococcus infections. However, because of antibiotic resistance in bacterial strains, treatment of skin infections is becoming increasingly difficult. The objective of this research is to study the effect of plant extract concentration on synthesis and morphology of biological silver nanoparticles and investigation of their activity against bacterial skin pathogens.

Methods: Biological silver nanoparticles were synthesized using two concentrations (5 and 10 ml) of Aegle marmelos fruit pulp extract. Ultraviolet (UV)-visible spectroscopy, field emission scanning microscopy (FESEM), and high resolution transmission electron microscopy (HRTEM) were used to analyze morphological features of nanoparticles. Antibacterial activity of synthesized silver nanoparticles was studied against the most common skin pathogens Staphylococcus aureus and Streptococcus pyogen, using a well diffusion method.

Results: The silver nanoparticles synthesized from 5 ml extract showed UV-absorbance peak at 430 nm with 14-18 nm size, while silver nanoparticles synthesized from 10 ml extract was showed the absorbance at 427 nm with 4-8 nm size. FESEM and HRTEM analysis revealed that both the silver nanoparticles were spherical in shape. Both nanoparticles have shown antibacterial activity among them silver nanoparticles synthesized from 10 ml extract showed better antibacterial activity.

Conclusion: This research confirms that plant extract concentration modulate the rate of synthesis, morphology, surface plasmon resonance, and activity of biological silver nanoparticles. Silver nanoparticles synthesized from 10 ml extract can be used efficiently in the treatment of bacterial skin infections.


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Author Biography

Patil Sunita, Department of Microbiology, School of Life Sciences, Karpagam University, Coimbatore - 641 021, Tamil Nadu, India.

principal Investigator (Women scientist Scheme-A) Department of Microbiology, School of Life Sciences, Karpagam University.


Sardana K, Mahajan S, Sarkar R, Mendiratta V, Bhushan P, KoranneRV, et al. The spectrum of skin disease among Indian children. Pediatr Dermatol 2009;26(1):6-13.

Palit A, Inamadar AC. Current concepts in the management of bacterial skin infections in children. Indian J Dermatol Venereol Leprol 2010;76(5):476-88.

Tomecki KJ. Common skin infections. Dis Manage 2010;2:1-6.

Perera G, Hay R. A guide to antibiotic resistance in bacterial skin infections. J Eur Acad Dermatol Venereol 2005;19(5):531-45.

Shah M, Fawcett D, Sharma S, Tripathy SK. Green synthesis of metallic nanoparticles via biological entities. Materials 2015;8(11):7278-308.

Anurag S, Sing HK, Pragati K, Ashutosh U. Bael (Aegle marmelos Correa) products processing: A review. Afr J Food Sci 2014;8(5):204-15.

Nirmala A, Manimekalai G, Vasanthi P, Jagajothi A, Evanjelene VK, Nadu T, et al. In vitro antimicrobial, phytochemical analysis of Marmelos fruit extracts. World J Pharm Pharm Sci 2016;5(7):803-11.

Gautam MK, Ghatule RR, Singh A, Purohit V, Gangwar M, Kumar M, et al. Healing effects of Aegle marmelos (L.) Correa fruit extract on experimental colitis. Indian J Exp Biol 2013;51(2):157-64.

Gnanajobitha G, Paulkumar K, Vanaja M, Rajeshkumar S, Malarkodi C, Annadurai G, et al. Fruit-mediated synthesis of silver nanoparticles using Vitis vinifera and evaluation of their antimicrobial efficacy. J Nano Chem 2013;3:67.

Priyaragini S, Sathishkumar SR, Bhaskararao KV. Biosynthesis of silver nanoparticles using actinobacteria and evaluating its antimicrobial and cytotoxicity activity. Int J Pharm Pharm Sci 2013;5 Suppl 2:709-12.

Parimala KS, Balaji S, Nithiyasoundari M. Synthesis of silver nanoparticles using different types of ornamental flower extracts and its antibacterial activity. Int J Curr Res 2015;7(8):18876-80.

Patil S, Sivaraj R, Rajiv P, Venckatesh R, Seenivasan R. Green synthesis of silver nanoparticle from leaf extract of Aegle marmelos and evaluation of its antibacterial activity. Int J Pharm Pharm Sci 2015;7(6):169-73.

Mani KM, Seethalakshmi S, Gopal V. Evaluation of in-vitro anti-inflammatory activity of silver nanoparticles synthesized using Piper nigrum extract. J Nanomed Nanotechnol 2015;6(2):1-5.

Rodríguez-León E, Iñiguez-Palomares R, Navarro RE, Herrera-Urbina R, Tánori J, Iñiguez-Palomares C, et al. Synthesis of silver nanoparticles using reducing agents obtained from natural sources (Rumex hymenosepalus extracts). Nanoscale Res Lett 2013;8(1):318.

Lino A, Deogracious O. The in-vitro antibacterial activity of Annona senegalensis, Securidacca longipendiculata and Steganotaenia araliacea - Ugandan medicinal plants. Afr Health Sci 2006;6(1):31-5.

Ndikau M, Noah NM, Andala DM, Masika E. Green Synthesis and Characterization of Silver Nanoparticles Using Citrullus lanatus Fruit Rind Extract. Int J Anal Chem 2017;2017:8108504.

Ahmed S, Ikram S. Silver nanoparticles: One pot green synthesis using Terminalia arjuna extract for biological application. J Nanomed Nanotechnol 2015;6(4):1-6.

Evanoff DD Jr, Chumanov G. Synthesis and optical properties of silver nanoparticles and arrays. Chemphyschem 2005;6(7):1221-31.

Tomaszewska E, Soliwoda K, Kadziola K, Tkacz-Szczesna B, Celichowski G, Cichomski M, et al. Detection limits of DLS and UV-Vis spectroscopy in characterization of polydispersity nanoparticles colloids. J Nanomater 2013;2013:10.

Maiti S, Krishnan D, Barman G, Ghosh SK, Laha JK. Antimicrobial activities of silver nanoparticles synthesized from Lycopersicon esculentum extract. J Anal Sci Technol 2014;5(1):40.

Hazarika D, Phukan A, Saikia E, Chetia B. Phytochemical screening and synthesis of silver nanoparticles using leaf extract of Rhynchotechum ellipticum. Int J Pharm Pharm Sci 2014;6(1):672-4.

Puchalski M, Dąbrowski P, Olejniczak W, Krukowski P, Kowalczyk P, Polański K. The study of silver nanoparticles by scanning electron microscopy, energy dispersive X-ray analysis and scanning tunnelling microscopy. Mater Sci 2007;25(2):473-8.

Sivakumar P, Karthika P, Sivakumar P, Muralidharan NG, Devendran P, Renganathan S. Bio-synthesis of silver nano cubes from active compound quercetin-3-o-β-dgalactopyranoside containing plant extract and its antifungal application. Asian J Pharm Clin Res 2013;6 Suppl 4:76-9.

Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Appl Nanosci 2015;6(5):1-12.

Sulaiman GM, Hussien HT, Saleem M. Biosynthesis of silver nanoparticles synthesized by Aspergillus flavus and their antioxidant, antimicrobial and cytotoxicity properties. Bull Mater Sci 2015;38(3):639-44.

Murugesan S, Bhuvaneswari S, Sivamurugan V. Green synthesis, characterization of silver nanoparticles of a marine red alga Spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;9(5):192-7.



How to Cite

Sunita, P., and M. Palaniswamy. “SIZE DEPENDENT APPLICATION OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES AGAINST BACTERIAL SKIN PATHOGENS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 10, Oct. 2017, pp. 192-5, doi:10.22159/ajpcr.2017.v10i10.19718.



Original Article(s)