QUANTITATIVE ESTIMATION AND ANTIMICROBIAL POTENTIAL OF ETHANOL EXTRACT OF DURIO ZIBETHINUS MURR. LEAVES

Sridevi Chigurupati; Jahidul Islam Mohammad; Shantini Vijayabalan; Narmatha Devi Vaipuri; Kesavanarayanan Krishnan Selvarajan; Appala Raju Nemala

doi:10.22159/ajpcr.2017.v10i9.19767

Authors

Sridevi Chigurupati Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia
Jahidul Islam Mohammad Department of Pharmacology, Faculty of Medicine, Cyberjaya University College of Medical Sciences, CUCMS, Cyberjaya, 63000, Malaysia.
Shantini Vijayabalan Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia
Narmatha Devi Vaipuri Department of Pharmaceutical Chemistry, Faculty of Pharmacy, AIMST University, Semeling, 08100 Bedong, Kedah, Malaysia
Kesavanarayanan Krishnan Selvarajan Department of Pharmacology & Toxicology, College of Pharmacy, University of Hail, Hail, Kingdom Saudi Arabia.
Appala Raju Nemala Department of Pharmaceutical Chemistry, Sultanâ€“Ulâ€“Uloom College of Pharmacy, Road No.3, Banjara Hills, Hyderabad-500 034, (A.P.), India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.19767

Keywords:

Durio zibethinus, Durian, Phytochemical, Quantitative analysis, Antimicrobial, Zone of inhibition

Abstract

Objectives: Current research is aimed to investigate the natural antimicrobial potential of Durio zibethinus murr. ethanol leaves extract (DZL).

Methods: DZL was subjected to the preliminary phytochemical screening along with quantitative analysis of phenols and flavonoids. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were recorded. The agar well diffusion method was used to measure the antibacterial activity against gram positive and gram negative bacteria. The microorganisms used for the study were the ATCC strains of Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Neisseria gonorrhoeae, Pseudomonas aeruginosa and Escherichia coli.

Results: DZL exhibited the highest MIC of 0.1mg/mL and MBC of 0.25 mg/mL against gram negative bacteria, Pseudomonas aeruginosa and Escherichia coli. At MIC of 0.1mg/mL, DZL displayed significant zone of inhibition against Pseudomonas aeruginosa and Escherichia coli compared to gentamycin.

Conclusion: This research has shown that DZL has natural antibacterial properties against gram negative human pathogens.

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