GREEN SYNTHESIS AND CHARACTERIZATION OF MARINE YEAST-MEDIATED SILVER AND ZINC OXIDE NANOPARTICLES AND ASSESSMENT OF THEIR ANTIOXIDANT ACTIVITY

Authors

  • Aswathy R Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India
  • Gabylis B Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India
  • Anwesha S Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India
  • Bhaskara Rao Kv Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i10.19979

Keywords:

Marine yeast, Silver nanoparticle, Zinc oxide nanoparticle, Scanning electron microscopy, Ultraviolet-spectophotometry, Fourier transform infra-red, Atomic force microscopy

Abstract

 

 Objective: The present study focuses on the synthesis of silver and zinc oxide (ZnO) nanoparticles from marine yeasts, isolated from the sediments of the Bay of Bengal, Bakkhali coast, West Bengal, and India.

Methods: The marine sediment samples were diluted through serial diution and cultured onto yeast malt agar medium by the spread plate method. The selected yeast isolates were screened for the biosynthesis of silver and ZnO nanoparticles. Characterization of both the nanoparticles was done by applying ultraviolet (UV)-visible spectroscopy, atomic force microscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy.

Results: A total of five marine yeasts isolates were able to synthesize silver and ZnO nanoparticles as evidence of the color change. Optical density was measured in UV-spectrometer at different time interval for the conformation of production of nanoparticles. The size of silver nanoparticle was 31.78 nm and ZnO nanoparticle was 86.27 nm. The synthesized nanoparticles are then used for antioxidant assays.

Conclusions: We are concluding that marine yeast isolates SAG1 and SAG2 both are potential marine yeast isolates which can synthesize both the silver and ZnO nanoparticles. They also showed good antioxidant activity.

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Author Biographies

Aswathy R, Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

Department of Biomedical Sciences,

School of Biosciences and Technology

Gabylis B, Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

Department of Biomedical Sciences,

School of Biosciences and Technology

Anwesha S, Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

Research Associate,

Department of Biomedical Sciences,

School of Biosciences and Technology

Bhaskara Rao Kv, Department of Biomedical Sciences, Molecular and Microbiology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

Associate Professor,

DEnvironmental Biotechnology Division,

School of Biosciences and Technology

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Published

01-10-2017

How to Cite

R, A., G. B, A. S, and B. R. Kv. “GREEN SYNTHESIS AND CHARACTERIZATION OF MARINE YEAST-MEDIATED SILVER AND ZINC OXIDE NANOPARTICLES AND ASSESSMENT OF THEIR ANTIOXIDANT ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 10, Oct. 2017, pp. 235-40, doi:10.22159/ajpcr.2017.v10i10.19979.

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