PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF AERIAL PART EXTRACTS OF PLANTAGO MAJOR L.

Jyoti Vandana; Gupta A K; Alok Mukerjee

doi:10.22159/ajpcr.2017.v10i9.20031

Authors

Jyoti Vandana Department of Chemistry, SHUATS, Allahabad, Uttar Pradesh, India.
Gupta A K Department of Forensic Science, SHUATS, Allahabad, Uttar Pradesh, India.
Alok Mukerjee Department of Pharmacognosy, United Institute of Pharmacy, Allahabad, Uttar Pradesh, India

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.20031

Keywords:

Phytochemical screening, Plantago major L, Anti-inflammatory activity, Carrageenan, Diclofenac sodium

Abstract

Â

Â Objective: This study aims to perform phytochemical screening and investigate anti-inflammatory activity of extract from aerial part of the plant Plantago major L.

Methods: Preliminary phytochemical screening of plant extract was carried out using standard qualitative procedures and anti-inflammatory activity was evaluated using carrageenan-induced paw edema model in male Wistar albino rats using plethysmometer. Diclofenac sodium (10 mg/kg, b.w.) was used as standard drug.

Results: Preliminary phytochemical screening of the different extracts revealed that they were rich in secondary metabolite compounds such as carbohydrates, tannins, steroids, flavonoids, terpenoids, glycosides, fats and oils, alkaloids, phenolic compounds, and iridoid glycosides. The anti-inflammatory activity was evaluated using carrageenan-induced paw edema models in Wistar albino rats. The anti-inflammatory activity of methanolic extract (ME) was found to be dose dependent in carrageenan-induced paw edema model. The ME has shown significant (p<0.05) inhibition of paw edema, 61.11% and 55.55% on 4th hr at the doses of 900 and 600 mg/kg, respectively.

Conclusion: The results of the present study demonstrate that ME of the aerial parts of the plant possess significant (p<0.05) anti-inflammatory potential.

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Author Biography

Jyoti Vandana, Department of Chemistry, SHUATS, Allahabad, Uttar Pradesh, India.

Assistant Professor

References

Simon MK, Nafarnda WD, Obeta SS. Iridoids glycosides isolated from Combretum molle stem bark aqueous methanol extract. Glob Vet 2012;8(3):237-43.

Nathan C. Points of control in inflammation. Nature 2002;420(6917):846-52.

Franklin PX, Pillai AD, Rathod PD, Yerande S, Nivsarkar M, Padh H, et al. 2-amino-5-thiazolyl motif: A novel scaffold for designing antiinflammatory agents of diverse structures. Eur J Med Chem 2008;43(1):129-34.

White M. Mediators of inflammation and the inflammatory process. J Allergy Clin Immunol 1999;103:S378-81.

Divyasree MS, Bhagyasri Y, Lavakumar V, Kumar CK. An overview on anti-inflammatory activity of Indian herbal plants. Int J Res Pharm Nano Sci 2015;4(1):1-9.

Albach DC, Meudt HM, Oxelman B. Piecing together the new Plantaginaceae. Am J Bot 2005;92(2):297-315.

Taskova R, Evstatieva L, Handjieva N, Popov S. Iridoid patterns of genus Plantago L. And their systematic significance. J Nat Res 2002;57(1-2):42-50.

Samuelsen AB. The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J Ethnopharmacol 2000;71(1-2):1-21.

Velazquez MM, Lanza AM, Matellano LF. Polyphenolic compounds from Plantago lagopus. J Nat Res 2000;55(11-12):877-80.

Reina E, Al-Shibani N, Allam E, Gregson KS, Kowolik M, Windsor LJ. The effects of Plantago major on the activation of the Neutrophil Respiratory Burst. J Tradit Complement Med 2013;3(4):268-72.

Tarvainen M, Suomela J, Kallio H, Yang B. Triterpene acids in Plantago major: Identification, quantification and comparison of different extraction methods. Chromatographia 2010;71(3):279-84.

Jamilah J, Sharifa AA, Sharifah NR. GC-MS analysis of various extracts from leaf of Plantago major used as traditional medicine. World Appl Sci J 2012;17:67-70.

Gomez-Flores R, Calderon CL, Scheibel LW, Tamez-Guerra P, Rodriguez-Padilla C, Tamez-Guerra R, et al. Immunoenhancing properties of Plantago major leaf extract. Phytother Res 2000;14(8):617-22.

Chiang LC, Chiang W, Chang MY, Ng LT, Lin CC. Antiviral activity of Plantago major extracts and related compounds in vitro. Antiviral Res 2002;55(1):53-62.

Chiang LC, Chiang W, Chang MY, Lin CC. In vitro cytotoxic, antiviral and immunomodulatory effects of Plantago major and Plantago asiatica. Am J Chin Med 2003;31(2):225.

Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5(11):1142-5.

Guillen ME, Emim JA, Souccar C, Lapa AJ. Analgesic and anti-inflammatory activities of the aqueous extract of Plantago major L. Int J Pharm 1998;35(2):99-104.

Beara IN, Lesjak MM, Jovin ED, Balog KJ, Anackov GT, Orcic DZ, et al. Plantain (Plantago L.) Species as novel sources of flavonoid antioxidants. J Agric Food Chem 2009;57(19):9268-73.

Burger GT, Miller CL. Animal care and facilities. In: Hayes AW, editor. Principle and Methods of Toxicology. 2nd ed. NY: Raven Press Ltd.; 1989. p. 527-31.

Goyal RK. Practical in Pharmacology. 3rd ed. Ahmedabad: Shah Prakashan Publishers; 2002.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998. p. 120.

Sofowora A. Medicinal Plants and Traditional Medicine in Africa. Vol. 2. New York: John Wily and Sons; 1993. p. 6-56.

Trease GE, Evans WC. Pharamacognosy. Vol. 14. London: W. B. Scandars Company Ltd.; 1989. p. 269-300.

Atta AH, Abo EL-Sooud K. The antinociceptive effect of some Egyptian medicinal plant extracts. J Ethnopharmacol 2004;95(2-3):235-8.

Atta AH, Nasr SM, Mouneir SM. Potential protective effect of some plant extracts against carbon tetrachloride-induced hepatotoxicity. Afr J Tradit Complement Altern Med 2006;3(3):1-9.

Ozaslan M, Karagoz ID, Kilic IH, Cengiz B, Kalender ME, Guldur ME, et al. Effect of Plantago major sap on Ehrlich ascites tumours in mice. Afr J Biotechnol 2009;8(6):955-9.

Barua CC, Pal SK, Roy JD, Buragohain B, Talukdar A, Barua AG, et al. Studies on the anti-inflammatory properties of Plantago erosa leaf extract in rodents. J Ethnopharmacol 2011;134(1):62-6.

Handa SS, Sharma A. Hepatoprotective activity of and rographolide from Andrographis paniculata against carbon tetrachloride. Indian J Med Res 1990;92:276-83.

Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for anti-iflammatory drugs. Proc Soc Exp Biol Med 1962;111:544-7.

Suralkar AA, Rodge NK, Kamble RD, Maske KS. Evaluation of anti-inflammatory and analgesic activities of Tamarindus indica seeds. Int J Pharm Sci Drug Res 2012;4(3):213-7.

Harris JM, Spencer PS. A modified plethysmographic apparatus for recording volume changes in the rat paw. J Pharm Pharmacol 1962;14:464-6.

SÃ¼leyman H, Demirezer LO, KuruÃ¼zÃ¼m A, Banoglu ZN, GÃ¶Ã§er F, Ozbakir G, et al. Antiinflammatory effect of the aqueous extract from Rumex patientia L. Roots. J Ethnopharmacol 1999;65(2):141-8.

Metivier RP, Francis FJ, Clydesdale FM. Solvent extraction of anthocyanins from wine pomace. J Food Sci 2006;45(4):1099-100.

Sonia RK, Irchhaiyaa R, Dixit V, Bhat ZA, Wani HA, Najar AH. Anti-inflammatory activity of Kirganelia reticulata (Poir). Baill. Root by carrageenan-induced rat paw oedema model. Int J Pharm Pharm Sci 2014;6(1):520-3.

Rahman MD, Sharmin RR, Nazimuddin MM, Zaman MU, Rana S, Ahmed N. Antinociceptive and anti-inflammatory effect of Crinum asiaticum bulb extract. Asian J Pharm Clin Res 2011;4(3):34-7.

Suba V, Murugesan T, Kumaravelrajan R, Mandal SC, Saha BP. Anti-inflammatory, analgesic and anti peroxidative efficacy of Barleria lupulina Lindl extract. Phytother Res 2005;19(8):695-9.

Niemegeers CJ, Verbruggen FJ, Janssen PA. Effect of various drugs on carrageenan induced oedema in the rats hind paw. J Pharmacol 1964;16:810-6.

Hussan F, Mansor AS, Hassan SN, Tengku Nor Effendy Kamaruddin TN, Budin SB, Othman F. Anti-inflammatory property of Plantago major leaf extract reduces the inflammatory reaction in experimental acetaminophen-induced liver injury. Evid Based Complement Alternat Med 2015;2015:347861.

TÃ¼rel I, Ozbek H, Erten R, Oner AC, Cengiz N, Yilmaz O. Hepatoprotective and anti-inflammatory activities of Plantago major L. Indian J Pharmacol 2009;41(10):120-4.

Wang JR, Zhou H, Jiang ZH, Wong YF, Liu L. In vivo anti-inflammatory and analgesic activities of a purified saponin fraction derived from the root of Ilex pubescens. Biol Pharm Bull 2008;31(4):643-50.

Garcia-Leme J, Nakamura L, Leite MP, Silva MR. Pharmacological analysis of the acute inflammation process induced in rats paw by local injection of carrageenan and heating, Br J Pharmacol 1973;64:91-8.

Viljoen A, Mncwangi N, Vermaak I. Anti-inflammatory iridous of botanical origin. Curr Med Chem 2012;19(14):2104-27.

Shim KM, Choi SH, Jeong MJ, Kang SS. Effects of aucubin on the healing of oral wounds. In Vivo 2007;21(6):1037-41.

Yam MF, Ang LF, Ameer OZ, Salman IM, Aziz HA, Asmawi MZ. Anti-inflammatory and analgesic effects of Elephantopus tomentosus ethanolic extract. J Acupunct Meridian Stud 2009;2(4):280-7.