• Murali Satyanarayana Bethu Department of Toxicology, Division of Pharmacology and Toxicology, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India.
  • Venkateswara Rao J Academy of Scientific and Innovative Research, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India.



Gloriosa superba, Silver nanoparticles, Characterization, Cytotoxicity



 Objective: We aimed to synthesize the cost effective, one pot and an eco-friendly technique for the green synthesis of silver nanoparticles (AgNPs) using 1 mM of silver nitrate (AgNO3) solution through the aqueous leaf extracts of Gloriosa superba (GS) reducing and capping agent and its anticancer activity.

Methods: Synthesis briefly 95 mL of 1 mM AgNO3 was taken into amber colored conical flask and added 5 mL of aqueous leaf extract of GS (pale brown) and incubated at room temperature in dark condition for about 24 hrs. Characterization of AgNPs derived from GS (GS-AgNPs) was performed with physiochemical techniques (ultraviolet, transmission electron microscope [TEM], X-ray diffraction [XRD], and thermal gravimetric analysis) and cytotoxicity by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide assay.

Results: We synthesized cost effective, eco-friendly AgNPs were characterized by physiochemical techniques. The crystal nature of AgNP was studied by XRD. TEM studies reveal the morphology of GS-AgNPs, the size of the nanoparticle is 10-50 nm. The cytotoxicity of GS-AgNPs studied against the four human cancerous cell line DU145, SKOV3, PC3, and A549 but the GS-AgNPs are most sensitive toward the SKOV3 cell line. The minimum inhibitory concentration (IC) is 79.45±5.26, 61.80±4.27, 94.74±9.26, and 90.10±8.24 μg/mL, respectively. Morphological assessment of the SKOV3 cells was studied using AO/EB and Hoechst staining at IC50 concentration.

Conclusion: The bio fabrication of the GS-AgNPs were simple, eco-friendly and one pot synthesis, it is used as an anticancer agent in future, pending further investigation.


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Author Biographies

Murali Satyanarayana Bethu, Department of Toxicology, Division of Pharmacology and Toxicology, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India.

Venkateswara Rao J, Academy of Scientific and Innovative Research, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India.

Cheif Scientist,

Pharmacology and Toxicology Division


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How to Cite

Bethu, M. S., and V. Rao J. “BIOFABRICATION OF SILVER NANOPARTICLES USING LEAVES OF GLORIOSA SUPERBA AND ITS ANTICANCER PROPERTIES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 11, Nov. 2017, pp. 65-69, doi:10.22159/ajpcr.2017.v10i11.20389.



Original Article(s)