BIOFABRICATION OF SILVER NANOPARTICLES USING LEAVES OF GLORIOSA SUPERBA AND ITS ANTICANCER PROPERTIES
Keywords:Gloriosa superba, Silver nanoparticles, Characterization, Cytotoxicity
Â Objective: We aimed to synthesize the cost effective, one pot and an eco-friendly technique for the green synthesis of silver nanoparticles (AgNPs) using 1 mM of silver nitrate (AgNO3) solution through the aqueous leaf extracts of Gloriosa superba (GS) reducing and capping agent and its anticancer activity.
Methods: Synthesis briefly 95 mL of 1 mM AgNO3 was taken into amber colored conical flask and added 5 mL of aqueous leaf extract of GS (pale brown) and incubated at room temperature in dark condition for about 24 hrs. Characterization of AgNPs derived from GS (GS-AgNPs) was performed with physiochemical techniques (ultraviolet, transmission electron microscope [TEM], X-ray diffraction [XRD], and thermal gravimetric analysis) and cytotoxicity by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: We synthesized cost effective, eco-friendly AgNPs were characterized by physiochemical techniques. The crystal nature of AgNP was studied by XRD. TEM studies reveal the morphology of GS-AgNPs, the size of the nanoparticle is 10-50 nm. The cytotoxicity of GS-AgNPs studied against the four human cancerous cell line DU145, SKOV3, PC3, and A549 but the GS-AgNPs are most sensitive toward the SKOV3 cell line. The minimum inhibitory concentration (IC) is 79.45Â±5.26, 61.80Â±4.27, 94.74Â±9.26, and 90.10Â±8.24 Î¼g/mL, respectively. Morphological assessment of the SKOV3 cells was studied using AO/EB and Hoechst staining at IC50 concentration.
Conclusion: The bio fabrication of the GS-AgNPs were simple, eco-friendly and one pot synthesis, it is used as an anticancer agent in future, pending further investigation.
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