INSECTICIDAL, ANTIBACTERIAL, AND ANTIRADICAL ACTIVITY OF NICOTIANA PLUMBAGINIFOLIA VIV. (SOLANACEAE)

Prashith Kekuda Tr; Raghavendra Hl; Rajesh Mr; Avinash Hc

doi:10.22159/ajpcr.2017.v10i11.20559

Authors

Prashith Kekuda Tr Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Shivamogga, Karnataka, India.
Raghavendra Hl Department of Biochemistry, School of Medicine, Wollega University, Nekemte, Ethiopia.
Rajesh Mr Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Shivamogga, Karnataka, India.
Avinash Hc Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Shivamogga, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.20559

Keywords:

Nicotiana plumbaginifolia, Maceration, Larvicidal, Aedes aegypti, Agar well diffusion, Free radical, 1, 1-Diphenyl-2-picrylhydrazyl, 2, 2-Azinobis 3-ethylbenzothiazoline 6-sulfonate

Abstract

Â

Â Objectives: Nicotiana plumbaginifolia Viv. belongs to the family Solanaceae. The present study was carried out to determine insecticidal, antibacterial, and antiradical activity of aerial parts of N. plumbaginifolia.

Methods: Extraction was carried out by maceration process using methanol. Insecticidal activity was assessed, in terms of larvicidal effect, against II and IV instar larvae of Aedes aegypti. Antibacterial activity was evaluated against a panel of 7 bacteria by agar well diffusion assay. Antiradical activity was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays.

Results: The extract was found to cause dose-dependent mortality of larvae of A. aegypti. The lethal concentration 50 value of extract for II and IV instar larvae was found to be 0.41 and 0.99 mg/ml, respectively. Extract was effective in inhibiting all bacteria. Gram-positive bacteria displayed marked susceptibility when compared to Gram-negative bacteria. Bacillus cereus and Escherichia coli were susceptible to highest and least extent, respectively. Extract scavenged both DPPH radicals and ABTS radicals dose dependently. Extract scavenged ABTS radicals more efficiently (inhibitory concentration [IC50] value 13.51 Î¼g/ml) when compared to DPPH radicals (IC50 value 17.43 Î¼g/ml).

Conclusions: The plant N. plumbaginifolia appears to be a promising resource for developing agents with insecticidal, antibacterial, and antiradical activity. The observed bioactivities could be attributed to the presence of phytochemicals which are to be isolated, characterized, and subjected for bioactivity determinations. The plant can be used to prevent arboviral diseases, infectious diseases, and oxidative damage.

Downloads

Download data is not yet available.

References

Rose RI. Pesticides and public health: Integrated methods of mosquito management. Emerg Infect Dis 2001;7(1):17-23.

Shaalan EA, Canyon D, Younes MW, Abdel-Wahab H, Mansour AH. A review of botanical phytochemicals with mosquitocidal potential. Environ Int 2005;31(8):1149-66.

Poopathi S, Tyagi BK. The challenge of mosquito control strategies: From primordial to molecular approaches. Biotechnol Mol Biol Rev 2006;1(2):51-65.

Vinayaka KS, Swarnalatha SP, Preethi HR, Surabhi KS, Kekuda PT, Sudharshan SJ. Studies on in vitro antioxidant, antibacterial and insecticidal activity of methanolic extract of Abrus pulchellus Wall (Fabaceae). Afr J Basic Appl Sci 2009;1(5-6):110-6.

Kaushik R, Saini P. Screening of some semi-arid region plants for larvicidal activity against Aedes aegypti mosquitoes. J Vector Borne Dis 2009;46(3):244-6.

Samidurai K, Mathew N. Bioassay guided fractionation and GC-MS analysis of Euphorbia lactea extract. Int J Pharm Pharm Sci 2014;6(4):344-7.

Ghosh A, Chowdhury N, Chandra G. Plant extracts as potential mosquito larvicides. Indian J Med Res 2012;135(5):581-98.

Singh A, Bhattacharya K, Chandra G. Efficacy of Nicotiana plumbaginifolia (Solanaceae) leaf extracts as larvicide against malarial vector Anopheles stephensi Liston 1901. Int J Pharm Bio Sci 2015;6(1):860-8.

Mallikarjuna PB, Rajanna LN, Seetharam YN, Sharanabasappa GK. Phytochemical studies of Strychnos potatorum L.f. - A medicinal plant. E J Chem 2007;4(4):510-8.

Doughari JH, Human IS, Bennade S, Ndakidemi PA. Phytochemicals as chemotherapeutic agents and antioxidants: Possible solution to the control of antibiotic resistant verocytotoxin producing bacteria. J Med Plants Res 2009;3(11):839-48.

Pandey AK, Kumar S. Perspective on plant products as antimicrobial agents: A review. Pharmacologia 2013;4(7):469-80.

Khan MS, Sayeed SH, Uddin MH, Akter L, Ullah MA, Jahan S, et al. Screening and evaluation of antioxidant, antimicrobial, cytotoxic, thrombolytic and membrane stabilizing properties of the methanolic extract and solvent-solvent partitioning effect of Vitex negundo bark. Asian Pac J Trop Dis 2013;3(5):393-400.

Ahmad M, Wajid M. Plants as potential source of antimicrobial agents. J Pharm Altern Med 2013;2(3):18-25.

Srivastava J, Chandra H, Nautiyal AR, Kalra SJ. Antimicrobial resistance (AMR) and plant-derived antimicrobials (PDAms) as an alternative drug line to control infections. 3 Biotech 2014;4(5):451-60.

Hossain MA, Latif MA, Sarker BC, Jahan N. Importance of some Bangladeshi ethnomedicinal plants: A review. Eur J Med Plants 2016;16(4):1-14.

Driss D, Kaoubaa M, Mansour RB, Kallel F, Abdelmalek BE, Chaabouni SE. Antioxidant, antimutagenic and cytotoxic properties of essential oil from Corchorus olitorius L. Flowers and leaf. Free Radic Antioxid 2016;6(1):34-43.

Raghavendra HL, Kekuda PT, Akarsh S, Ranjitha MC, Ashwini HS. Phytochemical analysis, antimicrobial and antioxidant activities of different parts of Pleocaulus sessilis (Nees) Bremek (Acanthaceae). Int J Green Pharm 2017;11(2):98-107.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4(8):118-26.

Maruthappan V, Shree SK. A report on the antioxidant activity of the powder of the entire plant of Phyllanthus reticulatus poir. Int J Green Pharm 2010;4(4):265-9.

Pavithra GM, Siddiqua S, Naik AS, Kekuda PT, Vinayaka KS. Antioxidant and antimicrobial activity of flowers of Wendlandia thyrsoidea, Olea dioica, Lagerstroemia speciosa and Bombax malabaricum. J Appl Pharm Sci 2013;3(6):114-20.

Krishnamoorthy K, Krishnaswamy T, Subramaniam P, Sellamuthu M. Quantification of phytochemicals and in vitro antioxidant potential of various solvent extracts of certain species of Acanthaceae. Int J Green Pharm 2014;8:58-64.

Singh KP, Daboriya V, Kumar S, Singh S. Antibacterial activity and phytochemical investigations on Nicotiana plumbaginifolia Viv. (Wild tobacco). Rom J Biol Plant Biol 2010;55(2):135-42.

Shahriar M, Chowdhury SA, Imam, MZ, Datta BK. Analgesic and neuropharmacological activities of methanol extract from the leaf of Nicotiana plumbaginifolia Viv. Bangladesh Pharm J 2015;18(2):85-9.

Ajaib M, Fatima S, Khan KM, Perveen S, Shah S. Nicotiana plumbaginifolia: A rich antimicrobial and antioxidant source. J Chem Soc Pak 2016;38(1):143-9.

Available from: http://www.florakarnataka.ces.iisc.ac.in/hjcb2/ herbsheet.php?id=4119&cat=1.

Nawaz MM, Hossain M, Karim M, Khan M, Jahan R, Rahmatullah M. An ethnobotanical survey of Rajshahi district in Rajshahi division, Bangladesh. Am Eur J Sustain Agric 2009;3(2):143-50.

Rothe SP. Exotic medicinal plants from West Vidarbha region of Maharashtra - III. J Ecobiotechnol 2011;3(9):11-3.

Sharma J, Gaur RD, Gairola S, Painuli RM, Siddiqi TO. Traditional herbal medicines used for the treatment of skin disorders by the Gujjar tribe of Sub-Himalayan tract, Uttarakhand. Indian J Tradit Knowl 2013;12(4):736-46.

Singh TT, Sharma HM, Devi AR, Sharma HR. Plants used in the treatment of piles by the scheduled caste community of Andro village in Imphal East district, Manipur (India). J Plant Sci 2014;2(3):113-9.

Sharma DL, Devi SL, Singh BL, Singh CT. Medicinal plants found in Imphal Valley used in treatments of various ailments. Int J Sci Res 2015;4(12):282-6.

Ansar A, Naresh L, Simon S. Effect of different plant extracts as antifungal agents against Alternaria alternata causing leaf blight disease of cabbage (Brassica oleracea). Int J Pharm Bio Sci 2015;6(2):176-81.

Shah AS, Khan RA, Ahmed M, Muhammad N. Hepatoprotective role of Nicotiana plumbaginifolia Linn. Against carbon tetrachloride-induced injuries. Toxicol Ind Health 2016;32(2):292-8.

Kekuda PT, Siddiqha A, Pushpavathi D, Vinayaka KS, Raghavendra HL. Radical scavenging, cytotoxic and antimicrobial activity of Flacourtia indica (Burm. f.) Merr. Med Health Sci Res J 2017;1(1):76-82.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.

Fankam AG, Kuiate JR, Kuete V. Antibacterial and antibiotic resistance modifying activity of the extracts from Allanblackia gabonensis, Combretum molle and Gladiolus quartinianus against gram-negative bacteria including multi-drug resistant phenotypes. BMC Complement Altern Med 2015;15:206.

Rios JL, Recio MC, Villar A. Screening methods for natural products with antimicrobial activity: A review of the literature. J Ethnopharmacol 1988;23(2-3):127-49.

Das K, Tiwari RK, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. J Med Plants Res 2010;4(2):104-11.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6(2):71-9.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26(2):211-9.

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compost Anal 2006;19:669-75.

Krishna MS, Nair JA. Antibacterial, cytotoxic and antioxidant potential of different extracts from leaf, bark and wood of Tectona grandis. Int J Pharm Sci Drug Res 2010;2(2):155-8.

Wan C, Yu Y, Zhou S, Liu W, Tian S, Cao S. Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures. Pharmacogn Mag 2011;7(25):40-5.

Shalaby EA, Shanab SM. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J Geo Mar Sci 2013;42(5):556-64.

Zhen J, Villani TS, Guo Y, Qi Y, Chin K, Pan MH, et al. Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves. Food Chem 2016;190:673-80.

Fidrianny I, Rahmiyani I, Wirasutisna KR. Antioxidant capacities from various leaves extracts of four varieties mangoes using DPPH, ABTS assays and correlation with total phenolic, flavonoid, carotenoid. Int J Pharm Pharm Sci 2013;5(4):189-94.

Indu H, Seenivasan R. In vitro antioxidant activity of selected seaweeds from southeast coast of India. Int J Pharm Pharm Sci 2013;5(2):474-84.