THE BIOCHEMICAL ALTERATION AND DNA DAMAGE IN RATS (RATTUS RATTUS) AFTER CHRONIC INTRAPERITONEALLY INJECTION TO PURIFIED MICROCYSTIN-LR FROM ANABAENA CIRCINALIS

Maher M Khadairi; Moayed Jy Al-amari; Ayad Mj Al-mamoori

doi:10.22159/ajpcr.2017.v10i11.20565

Authors

Maher M Khadairi Assistant professor, Director of CBRNSSD , University of Babylon
Moayed Jy Al-amari Department of Biology, College of Science, University of Babylon, Hillah, Iraq
Ayad Mj Al-mamoori Department of Biology, College of Science, University of Babylon, Hillah, Iraq

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.20565

Keywords:

Cyanobacteria, Purification, Microcystin-leucine arginine, Biochemical markers, DNA damage markers

Abstract

Â

Â Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.

Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay.

Results: The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose.

Conclusion: This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.

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Author Biography

Maher M Khadairi, Assistant professor, Director of CBRNSSD , University of Babylon

Assistant professor, Biology Dept.

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