EFFECT OF ETHANOLIC EXTRACT OF CYANOTIS CRISTATA LEAVES APPLIED TOPICALLY ON WOUND HEALING IN WISTAR RATS

Anurag Pathak; Smita Shenoy; Sushil Kiran; Avinash Arivazahan; Deepak Nayak; Naveen Kumar; Amod Tilak; Manjunath Shetty

doi:10.22159/ajpcr.2017.v10i12.20731

Authors

Anurag Pathak Postgraduate, Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka - 576104
Smita Shenoy Additional Professor, Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka â€“ 576104
Sushil Kiran Postgraduate, Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka - 576104
Avinash Arivazahan Postgraduate, Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka - 576104
Deepak Nayak Associate Professor, Department of Pathology, Kasturba Medical College, Manipal, Manipal University, Karnataka - 576104
Naveen Kumar Assistant Professor, Department of Anatomy, Melaka Manipal Medical College, Manipal, Manipal University, Karnataka - 576104
Amod Tilak Postgraduate, Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka - 576104
Manjunath Shetty Lecturer, Department of Pharmacology, Melaka Manipal Medical College, Manipal, Manipal University, Karnataka â€“ 576104

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.20731

Keywords:

Incision wound, Excision, Burn wound, Breaking strength, Contraction, Epithelialization

Abstract

Â

Â Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats.

Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5%, 1%, and 2%, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey's post hoc test was used to analyze data.

Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control.

Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.

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