QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITIES OF SOME CITRUS FRUITS OF SOUTH INDIA

Pallavi M; Ramesh Ck; Krishna V; Sameera Parveen; Nanjunda Swamy L

doi:10.22159/ajpcr.2017.v10i12.20912

Authors

Pallavi M Post-graduate Department of Studies and Research in Biotechnology, Molecular Biomedicine Laboratory, Sahyadri Science College, Kuvempu University, Shimoga, Karnataka, India.
Ramesh Ck Post-graduate Department of Studies and Research in Biotechnology, Molecular Biomedicine Laboratory, Sahyadri Science College, Kuvempu University, Shimoga, Karnataka, India.
Krishna V Post-graduate Department of Studies and Research in Biotechnology and Bioinformatics, Jnana Sahyadri, Kuvempu University, Shimoga, Karnataka, India.
Sameera Parveen Post-graduate Department of Studies and Research in Biotechnology, Molecular Biomedicine Laboratory, Sahyadri Science College, Kuvempu University, Shimoga, Karnataka, India.
Nanjunda Swamy L Department of Academic Staff College, University of Mysore, Manasa Gangothri, Mysore, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.20912

Keywords:

Citrus fruits, Peel, Pulp, Qualitative and quantitative, In vitro antioxidant activity

Abstract

Objective: The current investigation was contemplated to evaluate the phytochemicals and in vitro antioxidant activities in peel and pulp of some commercially grown citrus fruits of South India, namely, lemon (Citrus aurantifolia), orange (Citrus reticulata), sour orange (Citrus aurantium), pomello (Citrus grandis), and citron (Citrus medica).

Methods: The peel and pulp of the fruits were separated and subjected to cold extraction using 70% alcohol. The extracts obtained were screened for the presence of their phytoconstituents using various qualitative and further quantified for major constituents. Further, the in vitro antioxidant activity was assayed by different radical scavenging methods, namely, 2,2-diphenyl-1-picrylhydrazyl, superoxide anion, nitric oxide, lipid peroxidation inhibition, iron chelating activity, and reducing power assay at different concentrations.

Results: All the citrus fruits have shown significant in vitro antioxidant activity for the parameters assessed, wherein peel extracts recorded superior antioxidant potential than their corresponding pulps. The broad range of activity of the extracts suggests that multiple mechanisms mediated by the phytoconstituents are responsible for the antioxidant activity.

Conclusion: The study thus revealed that peel and pulp of citrus fruits are potential sources of bioactive compounds which are reflected in antioxidant activity and supports their health-promoting claims of plethora of investigations.

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Author Biography

Pallavi M, Post-graduate Department of Studies and Research in Biotechnology, Molecular Biomedicine Laboratory, Sahyadri Science College, Kuvempu University, Shimoga, Karnataka, India.

Biotechnology

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