EFFECT OF ETHANOLIC EXTRACT OF CINNAMOMUM TAMALA LEAVES ON WOUND HEALING IN STZ INDUCED DIABETES IN RATS.
Abstract
Objective: Diabetes is a chronic hyperglycemic metabolic disorder; leads to generates many complications including delay in wound healing after any injury. These non healing wound ends upto organ or limb salvage. The available modern allopathic medications are not able to full control over these complications. There are several evidences that complication associated with diabetes can easily be treated by using herbal or folklore medicines. The leaves of Cinnamomum tamala used by traditional peoples in the treatment of hyperlipidemia, diabetes, and associated wound healing problems. The aim of our study was to find the active extract from various extracts of C. tamala leaves responcible for wound healing activity in diabetic rats.
Methods: The wistar albino rats were made diabetic by single i.p. injection of streptozotocin (60 mg/kg). The excision, incision and dead space wound were created on back side of rats. The ethanolic extract of C. tamala leaves of ethanolic extract of leaves of Cinnamomum tamala was applied topically in excision wound model while in incision and dead space wound model the ethanolic extract of C. tamala leaves (100 mg/kg) was give orally for 16 days.
Results: In the excision wound model the wound area and day of epithelization both were significantly decreased in ethanolic extract of C. tamala leaves treated rats. In incision wound model the significantly higher tensile strength was observed in rats treaded orally with ethanolic extract treated. There were significant increase in weight of wet & dry granulation tissue with increased amount of hydroxyproline, collagen and elastin was observed in treated rats by ethanolic extract of C. tamala leaves
Conclusion: The results suggested that the ethanolic extract of leaves of Cinnamomum tamala can be used in treatment of dalayed wound healing in diabetic rats.
Â
Key Words: Cinnamomum tamala, Collagen, Diabetes, Granulation, Hydroxyproline, Tensile strength, Wound healing
Â
Downloads
References
Zimmet PZ. Diabetes epidemiology as a tool to trigger diabetes research and care
Diabetologia. 1999;42: 99-518.
Teixeira CC, Rava CA, DaSilva PM, Melchior R, Argenta R, Anselmi F, Almeida CR, Fuchs FD. Absence of antihyperglycemic effect of jambolan in experimental and clinical models. J Ethnopharmacol. 2000;71(1-2):343-347.
Goodson WH, Hunt TK, Wound healing and the diabetic patient. Surg Gynecol Obstet. 1979;149:600-608.
Lien YH, Tseng MM, Stern R., Glucose and glucose analogs modulate collagen metabolism. Exp Mol Pathol. 1992;57:215–219.
Raghow R, The role of the extracellular matrix in postinflammatory wound healing and fibrosis. Fed Am Soc Exp Biol J. 1994;8:823-831.
The WHO Expert Committee on Diabetes Mellitus. Technical Report Series 646. Geneva: World Health Organization, 1980.
Seifter E, Rettura G, Pedawer J, Stratford F, Kambosos D, Levenson SM. Impaired wound healing in streptozotocin diabetes. Prevention by supplemental vitamin A. Ann Surg. 1981;194(1):42-50.
Kumudhavalli MV, Jaykar B. Evaluation of Antidiabetic activity of Costus igneus(L) leaves on STZ induced diabetic rats. Der Pharmacia Sinica. 2012;3(1):1-4.
Devender R K, Shashidher B, Kumar GP, Evaluation of wound healing activity of methanolic root extract of Plumbago zeylanica L. in wistar albino rats Asian J Plant Sci Res. 2011;1(2):26-34.
Sushil SP, Yogesh TS, Chetan AC, Lalit PS, Naveenkumar PJ, Chhaya HG, Wound healing activity of topical application form based on herbomineral formulation. Asian J Plant Sci Res. 2012;2 (3):355-363.
Nayak BS, Anderson M, Periara LM, Evaluation of wound-healing potential ofCatharanthus roseus leaf extract in rats Fitoterapia, 2007;78: 540-544.
Malan R, Walia A, Saini V, Gupta S, Comparison of different extracts leaf of Brassica juncea Linn on wound healing activity. Euro J Exp Bio. 2011;1(2):33-40.
Nayak BS, Pereira L, Muhraj D, Wound healing activity of Carica papaya L.in experimentally induced diabetic rats. Indian J Exp Biol. 2007;45:739-743.
Ambuj N, Nilesh G, Umesh KJ, Evaluation of Wound healing activity of Tinospora cordifolia Willd. Der Pharmacia Sinica. 2012;3(1):126-130.
Prasad V, Jain V, Girish D, Dorle AK, Wound-healing property of Momordica charantia L. fruit powderJ Herb Pharmacother. 2006; 6(3-4):105-115.
Reddy KG, Enwemeka SC, A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem, 1996;29:225-229.
Klein LR, Wesis PH, Induced connective tissue metabolism in vivo: reutilization of pre-existing collagen. Proc Nat Acad Sci USA, 1966;56(1):277-284.
Grant RA, Estimation of hydroxyproline by the AutoAnalyser. J Clin Pathol, 1964;17 :685-686.
Guo S, Dipietro LA, Factors affecting wound healing. J Dental Res, 2010;89(3):219.
King L, Impaired wound healing in patients with diabetes Nurs Stand. 2001;15(38):39.
Mrowicz R, Tannins: the new natural antioxidants. Eur J Lipid Sci Technol. 2007;109:549.
Smerq J, Sharma M, Possible Mechanism of Murraya koenigii and Cinnamomum tamala
With Reference to Antioxidants Activity Int J Pharm Sci Drug Res. 2011;3(3):260-264.
Palanisamy P, Srinath KR, Yoganand KD, Choudhary PC, Evaluation of antioxidant and antidiabetic activities of Cinamomum tamala leaves in streptozotocin induced diabetic rats. Int J Pharm, 2011;2(12):157-162.
Sharma SR, Dwivedi SK, Swarup D, Hypoglycemic and hypolipidemic effects of Cinnamomum tamala Nees leaves. Indian J Exp Biol, 1996;34(4):372-374.
Kar A, Choudhary BK, Bandhopadhyay NG, Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats J Ethnopharmacol, 2003; 84(1):105-108.
Rosenthal, SP, Acceleration of primay wound healing by insulin. Archives in Surgery 1968;96:53–55.
Albertson S, Hummel RP, Breeden M, Greenhalgh DG, Surgery, 1993;114(2): 368.
Raghow R, The role of the extracellular matrix in postinflammatory wound healing and fibrosis. Fed Am Soc Exp Biol J. 1994;8:823-831.
Galeano M, Torre V, Deodato B, Campo GM, Sturiale A, Squadrito F, Cavallari V, Cucinotta D, Buemi M, Altavilla D, Surgery. 2001;129:467.
P. Chithra, G.B. Sajithlal, Gowri Chandrakasan. Influence of aloe vera on the healing of dermal wounds in diabetic rats. Journal of Ethnopharmacology 1998;59:195–201.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.