CYTOTOXIC EFFECT FROM ETHYL ACETATE-METHANOL SUBFRACTION OF CARRISA CARANDAS L TOWARD HELA CELLS BY IN VITRO TEST

MAMIK P. RAHAYU; Reslely Harjanti; Mae S. H. Wahyuningsih; Supargiyono .

doi:10.22159/ajpcr.2017v10s3.21355

Authors

MAMIK P. RAHAYU Faculty of Pharmacy, Setia Budi University, Central of Java, Indonesia
Reslely Harjanti Faculty of Pharmacy, Setia Budi University, Central of Java, Indonesia
Mae S. H. Wahyuningsih Center for Tropical Medicine, Gadjah Mada University, Yogyakarta, Indonesia
Supargiyono . Center for Tropical Medicine, Gadjah Mada University, Yogyakarta, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017v10s3.21355

Keywords:

Carandas leaves (Carissa carandas L), Cytotoxic, MTT assay, sub Fraction

Abstract

Objective: Cervical cancer is a malignant type of cancer, often affects women, particularly in developing countries. Carrisa carandas leaves contained many secondary metabolites that had potency as an anticancer. The purpose of this study was to understand the cytotoxic effect of subfraction of Carrisa carandas leaves against HeLa cells.

Methods: Chloroform fraction was separated by VLC gradually with n-hexaneâ€“chloroformâ€“ethyl acetate and methanol. The same profiles from eluent chloroformâ€“ethyl acetate composed fraction 18-26 were categorized as Fr4 and ethyl acetate-methanol composed fraction 27-30 as Fr5. The cytotoxic effect was evaluated by MTT assay on HeLa cells

Results: The result showed that the cytotoxic effect of subfraction Fr4 and Fr5 had IC₅₀ values of 177 mg/ml and 98 mg/ml, respectively. Colorless crystal of Subfraction Fr 5-3 had IC₅₀ value of 333 mg/ml. Subfraction Fr 5 showed effective cytotoxic activity than the others. Conclusion: It had chemo-preventive effect against cancer cells

Conclusion: This study applied MTT (Microculture Tetrazolium) method by in vitro test. The advantages of this method are relatively rapid, sensitive and accurate

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