EVALUATION OF AQUEOUS AND ETHANOLIC EXTRACTS OF SYZYGIUM CARYOPHYLLATUM FOR ANTIBACTERIAL ACTIVITIES

Raghunath Pendru; Jagan Nadipelly; Jyothinath Kothapalli; Subbannayya Kotigadde

doi:10.22159/ajpcr.2018.v11i2.21687

Authors

Raghunath Pendru Department of Microbiology, School of Medicine, Texila American University, Guyana, South America.
Jagan Nadipelly Department of Pharmacology,School of Medicine, Texila American University, Guyana, South America.
Jyothinath Kothapalli Department of Anatomy, Maheshwara Medical College and Hospital, Chitkul, Isnapur, Patancheru, Telangana. http://orcid.org/0000-0002-3570-6068
Subbannayya Kotigadde Department of Microbiology, K.V.G Medical College and Hospital, Sullia, Karnataka.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.21687

Keywords:

Syzygium caryophyllatum, Antimicrobial property, Time-kill assay, Minimum inhibitory concentration

Abstract

Â Objective: It has been well documented that traditional medicinal plants confer considerable antimicrobial activity against various microorganisms.

Methods: The present study was designed to evaluate the antimicrobial properties of aqueous and ethanolic extracts of the leaves of Syzygium caryophyllatum against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, and Vibrio cholerae. The aqueous extract was prepared using cold percolation method, and ethanolic extract was prepared in Soxhlet apparatus using ethyl alcohol. Antimicrobial activity is being determined by time-kill assay and minimum inhibitory concentration.

Results: From our study, it is found that all tested organisms were sensitive to both aqueous and ethanolic extract of leaves of S. caryophyllatum. The ethanolic extract showed better antibacterial activity against S. aureus and S. typhi when compare to aqueous extract. The phytochemical analysis confirmed the presence of flavonoids, alkaloids, glycosides, steroids, phenols, tannins, and saponins in the ethanolic and aqueous extract of S. caryophyllatum.

Conclusion: We hypothesize that these active phytoconstitutents might be responsible for the antimicrobial activity of this plant.

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Author Biographies

Raghunath Pendru, Department of Microbiology, School of Medicine, Texila American University, Guyana, South America.

Department of Microbiology

Jagan Nadipelly, Department of Pharmacology,School of Medicine, Texila American University, Guyana, South America.

Department of Pharmacology

Jyothinath Kothapalli, Department of Anatomy, Maheshwara Medical College and Hospital, Chitkul, Isnapur, Patancheru, Telangana.

Assistant professor, Department of Anatomy

Subbannayya Kotigadde, Department of Microbiology, K.V.G Medical College and Hospital, Sullia, Karnataka.

Department of Microbiology

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