THE EVALUATION OF ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF THE ESSENTIAL OIL EXTRACTED FROM THE AERIAL PARTS OF SOUTHERNWOOD HERB (ARTEMISIA ABROTANUM L.) THAT RECENTLY GROWN IN IRAQ

Sarah S Almahdawy; Ali Muaffaq Said; Ibrahim S Abbas; Ashour H Dawood

doi:10.22159/ajpcr.2017.v10i10.21725

Authors

Sarah S Almahdawy Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Al-Mustansiriya University, Baghdad, Iraq.
Ali Muaffaq Said Department of Pharmacy, Al-Yarmouk University College, Baghdad, Iraq.
Ibrahim S Abbas Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Al-Mustansiriya University, Baghdad, Iraq
Ashour H Dawood Department of Pharmaceutical Chemistry, College of Pharmacy, Al-Mustansiriya University, Baghdad, Iraq

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i10.21725

Keywords:

Southernwood, Artemisia abrotanum, Human rhabdomyosarcoma cell, (3-(4, 5-dimethyl-2-thiazolyl)-2, 5 diphenyl-2H tetrazolium bromide)

Abstract

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Â Objective: This research is to study the assessment of the antimicrobial and cytotoxic activity of the essential oil extracted from the aerial parts of Artemisia abrotanum L. that recently grown in Iraq.

Methods: The essential oil of A. abrotanum was extracted by hydrodistillation using Clevenger apparatus. This essential oil was tested for antimicrobial activity of five different pathogenic microorganisms (Gram-positive [Staphylococcus aureus and Bacillus subtilis] and Gram-negative [Salmonella typhi and Escherichia coli] bacterial strains) and fungi: Candida albicans using diffusion well agar method. Furthermore, this essential oil was tested for cytotoxic activity using rhabdomyosarcoma cell line, and the growth or inhibition of cancer cells was measured by MTT method.

Results: The obtained results show that the antibacterial activity for A. abrotanum against S. aureus was at concentrations 40, 25, and15 Î¼l with minimum inhibitory concentrations of 20 mm, while it showed antibacterial activity against E. coli for four different concentrations of 40, 25, 15, and10 Î¼l with inhibition zone of 16, 12, 14, and 10 mm, respectively, and it showed antifungal activity against C. albicans at four concentrations 40, 25, 15, and10 Î¼l with inhibition zone of 18, 24, 26, and 30 mm, respectively. The cytotoxic activity of the extracted essential oil was showed that the three concentrations of the extract (25, 50, and 100 Î¼g/ml) were all lower significantly as compared to dimethyl sulfoxide group. A significant difference was seen for group 25 with both groups 50 and 100, but no significant difference was seen between the two later. Finally, the antimicrobial and anticancer activity of this plant could be due to its essential oil constituents: Borneol, cymene, camphor, terpineol, eucalyptol, and aromadendrene.

Conclusion: The essential oil of A. abrotanum L. has a potent antimicrobial and anticancer effect against the tested microbial organisms and the cancer cells.

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Author Biography

Sarah S Almahdawy, Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Al-Mustansiriya University, Baghdad, Iraq.

phrmacognosy and medicinal plants department

References

Hussien HA, Hussein MS, Tkachenko KG, Nkomo M, Mudau FN. Essential oil composition of A. vulgaris grown in Egypt. Int J Pharm Pharm Sci 2016;8(9):120-3.

Amirmohammadi M, Khajoenia S, Bahmani M, Kopaei MR, Eftekhari Z, Qorbani M. In vivo evaluation of antiparasitic effects of Artemisia abrotanum and Salvia officinalis extracts on Syphacia obvelata, Aspiculoris tetrapetra and Hyenolepis nana parasites. Asian Pac J Trop Dis 2014;4(1):250-4.

Ibrahim NM. Extraction and characterization of Iraqi Artemisia dracunculus dried aerial parts extract though HPLC and GC-MS analysis with evaluation of its antitumor activity against 7, 12-dimethyl benzene (a) anthracene induced skin cancer in mice. Int J Pharm Pharm Sci 2017;9(5):34-2.

Coelho JP, Cristion AF, Matos PG, Ratuter AP, Nober BP, Mendes RL, et al. Extraction of volatile oil from aromatic plants with supercritical carbon dioxide: Experiments and modeling. Molecules 2012;17(9):10550-73.

Suresh J, Reddy AV, Rajan D, Ihsanullah M, Khan MN. Antimicrobial activity of Artemisia abrotanum and Artemisia pallens. Int J Pharm Pharm Res 2011;3(2):18-21.

Suganya S, Bharathidasan R, Senthilkumar G, Madhanraj P, Panneerselvam A. Antibacterial activity of essential oil extracted from Coriandrum sativum (L.) and GC-MS analysis. J Chem Pharm Res 2012;4(3):1846-50.

Gordanian B, Behbahani M, Carapetian J, Fazilati M. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species. Res Pharm Sci 2014;9(2):91-6.

Sadeghi H, Ghasemi N, Kohi M. Cytotoxic effect of Convolvulus arvensis extracts on human cancerous cell line. Res Pharm Sci 2008;3(1):31-4.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1-2):55-63.

Berridge MV, Tan AS. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys 1993;303(2):474-82.

Obistioiu D, Cristina RT, Schmerold I, Chizzola R, Stolze K, Nichita L, et al. Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris. Chem Central J 2014;8(6):1-11.

Alama HE, El-Aissami1 AE, Said AA, Alaoui-Faris FE. Evaluation of anti - Candida albicans activity of essential oils of six medicinal plants: Synergy concept. J Chem Pharm Res 2015;7(10):281-4.

Mokbel AA, Alharbi AA. Antifungal effects of basil and camphor essential oils against Aspergillus flavus and A. parasiticus. Aust J Crop Sci 2015;9(6):532-7.

Bayala B, Bassole IH, Scifo R, Gnoula C, Morel L, Lobaccaro JM, et al. Anticancer activity of essential oils and their chemical components - A review. Am J Cancer Res 2014;4(6):591-607.

Kang SH, Kim EK, Kim YS, Hwang JW, Jeong JH, Dong X, et al. Anticancer activity of thymol on AGS human gastric carcinoma cells. J Microb Biotechnol 2016;26(1):28-37.

Sobral MV, Xavier AL, Lima TC, De Sousa DP. Antitumor activity of monoterpenes found in essential oils. ScientificWorldJournal 2014;2014:953451.

Xu GL, Geng D, Xie M, Teng KY, Tian YX, Liu ZZ, et al. Chemical composition, antioxidative and anticancer activities of the essential Oil: Curcumae rhizoma-Sparganii rhizoma, a traditional herb pair. Molecules 2015;20(9):15781-96.