SCREENING OF ANTIOXIDANT PROPERTY IN MEDICINAL PLANTS BELONGING TO THE FAMILY APOCYNACEAE ZAHRA

Authors

  • Zahra Iqbal Department of Botany, University of Lucknow, Lucknow - 226 007, Uttar Pradesh, India.
  • Mohammed Shariq Iqbal Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow - 226 028, Uttar Pradesh, India.
  • Kumkum Mishra Department of Botany, University of Lucknow, Lucknow - 226 007, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.22303

Keywords:

Apocynaceae, Antioxidant, Catharanthus roseus, Carotene, Flavonoid, Phenolic

Abstract

Objective: The present study was aimed to explore antioxidant properties of the plants belonging to the family Apocynaceae.

Methods: Plants used in the study were Alstonia scholaris, Calotropis gigantea, Catharanthus roseus, Cerbera odollam, and Wrightia tinctoria. The plant material was used to estimate non-enzymatic antioxidant, namely, total antioxidant, total flavonoids, total phenolic, and total carotene content using spectrophotometer.

Result: The results showed significant antioxidant potential of the plants used in the study. It was observed that total flavonoids content was maximum in W. tinctoria (65.21 ± 3.712 mg equivalent quercetin/g of tissue), while total phenolic content was found maximum in C. roseus (59.63 ± 2.151 mg equivalent gallic acid/g of tissue). When total carotene content and total antioxidant activity were estimated, it was found maximum in C. roseus with (70.91 ± 4.41 μg/g fresh weight) and (0.846 ± 0.109 mM equivalent ascorbic acid/g tissue), respectively.

Conclusion: It was observed C. roseus possess potent antioxidant property compared with other plants of same family. When correlation was established between the antioxidants and different genera plants, a strong positive correlation was observed, indicating that the plants belonging to the family Apocynaceae are a potent source of antioxidants. Further research is recommended to explore this family (Apocynaceae) and to fully elucidate its biochemical (antioxidant) benefits.

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Author Biographies

Zahra Iqbal, Department of Botany, University of Lucknow, Lucknow - 226 007, Uttar Pradesh, India.

Department of Botany

Mohammed Shariq Iqbal, Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow - 226 028, Uttar Pradesh, India.

Amity Institute of Biotechnology

Kumkum Mishra, Department of Botany, University of Lucknow, Lucknow - 226 007, Uttar Pradesh, India.

Professor Department of botanyUniversity of Lucknow

References

Madhuri S, Pandey G. Some anticancer plants of foreign origin. Curr Sci 2009;96:6.

Peteros NP. Antioxidant and cytotoxic activities and phytochemical screening of four Phillppine medicinal plants. J Med Plant Res 2010;4:407-14.

Agbafor KN, Nwachukwu N. Phytochemical analysis and antioxidant property of leaf extracts of Vitex doniana and Mucuna pruriens. Biochem Res Int 2011;2011:459839.

Davies MJ. Identification of a globin free radical in equine myoglobin treated with peroxides. Biochim Biophys Acta 1991;1077:86-90.

Halliwell B, AruomaOI. DNA and Free Radicals. Chichester, England: Ellis Horwood, Division of Simon and Schuster Intl.; 1993.

Sies H. Glutathione and its role in cellular functions. Free Radic Biol Med 1999;27:916-21.

Sies H, Stahl W, Sundquist AR. Antioxidant functions of vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Ann N Y Acad Sci 1992;669:7-20.

Endress ME and Bruyns PV. A revised classification of the apocynaceae s.l. Bot Rev 2000;66:1-56.

Phillipson JD. Phytochemistry and pharmacognosy. Phytochemistry 2007;68:2960-72.

Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001;109 Suppl 1:69-75.

Soejarto DD. Ethnographic component and organism documentation in an ethnopharmacology paper: A minimum†standard. J Ethnopharmacol 2005;100:27-9.

Rapanarivo SH, Leeuwenberg AJ. Taxonomic revision of Pachypodium. Series of Revisions of Apocynaceac XLVIII. In: Rapanarivo SH, editor. Pachypodium (Apocynaceae): Taxonomy, Habitats and Cultivation. Rotterdam: Balkema; 1999. p. 1-82.

Kevin AL, Emmanuel G, Ellen P. Flavonoid Quantification in onion by spectrophotometric and high performance liquid chromatography analysis. Hortscience 2002;37:682-5.

Iqbal MS, Ansari MI, JafriS, PadmeshS, Ahmad I, Pandey B. Antioxidant potential of some medicinal plants (Ocimum sanctum, Azadirachta indica and Nigella sativa). Pharm Int Res J 2014;5:631-7.

Ragaa ES, Alaa SA, Mohammed AA, Ayman AG, Amira AA. Determination of phenolic components and antioxidant activity of some egyptian tea samples. Int J Pharm Pharm Sci 2015;7:198-202.

Arindam AG, Nishita DB, Anisha AK, Sneha SM, Michelle RM, et al. Antiproliferative, antioxidant activity and total phenolic content of Mitragyna parvifolia (roxb.) Korth. Int J Pharm Pharm Sci 2014;6:632-7.

Sükran D, Tohit G, Rıdvan S. Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents Tr. J Bot 1998;22:13-7.

Lichtenthaler HK, Wellburn AR. Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Biol Soc Trans 1985;11:591-2.

Cacig S, Szabo MR, Lupea AX, Ardelean A. Determination of the antioxidant activity of Ziziphus jujuba and Hydrangea incognita aqueous extracts. Studia Univ Vasile Goldis Seria St Vietii 2005;15:69-72.

Ayoola GA, Folawewo AD, Adesegun SA, Abioro OO, Adepoju-Bello AA, Coker HA. Phytochemical and antioxidant screening of some plants of Apocynaceae from South West Nigeria. Afr J Plant Sci

;2:124-8.

Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999;47:3954-62.

Natali RM, Robert V. Phenolic compounds in Catharanthus roseus. Phytochem Rev 2007;2:243-58.

Punia S, Jagjit K, Raman K, Kuldeep K. A medidinal plant with potent antitumor properties. Int J Res Ayurveda Pharm 2014;5:652-6.

Paiva SA, Russell RM. Beta-carotene and other carotenoids as antioxidants. J Am Coll Nutr 1999;18:426-33.

Rasool N, Rızwan K, Zubaır M, Kaleem RN, Imran I, Viqar-uddin A. Antioxidant potential of different extracts and fractions of Catharanthus roseus shoots. Int J Phytomed 2011;3:108-14.

Bhutkar MA, Bhise SB. Comparative studies on antioxidant properties of Catharanthus rosea and Catharanthus alba. Int J Pharmtech Res 2011;3:1551-6.

Published

01-12-2017

How to Cite

Iqbal, Z., M. S. Iqbal, and K. Mishra. “SCREENING OF ANTIOXIDANT PROPERTY IN MEDICINAL PLANTS BELONGING TO THE FAMILY APOCYNACEAE ZAHRA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 12, Dec. 2017, pp. 415-8, doi:10.22159/ajpcr.2017.v10i12.22303.

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