EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ISOLATED CONSTITUENTS FROM AREAL PART OF CUSCUTA REFLEXA ROXB. PLANT

Sandeep; Abhilasha Mittal

doi:10.22159/ajpcr.2018.v11i3.22475

Authors

Sandeep Department of Pharmacognosy, Jayoti Vidyapeeth Womenâ€™s University, Jaipur â€“ 303 122, Rajasthan, India.
Abhilasha Mittal Faculty of Pharmaceutical Science, Jayoti Vidyapeeth Womenâ€™s University, Jaipur - 303122, Rajasthan, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i3.22475

Keywords:

Cuscuta reflexa Roxb, Antimicrobial activity, In vitro antioxidant activity

Abstract

Â Objective: The objective of the study was to investigate the pharmacological evaluation of previously isolated compounds (CR-1 to CR-5) from the areal part of Cuscuta reflexa Roxb. is reported.

Methods: The antimicrobial and antioxidant activity of the isolated compounds (CR-1 to CR-5) from C. reflexa was determined by the disc-diffusion method and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) model, respectively. The antimicrobial activity was performed against four strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa.

Results: The results revealed that highest zone of inhibition is measured by compound CR-5 against E. coli. The antioxidant activity is evaluated for in vitro antioxidant activity using DPPH radical scavenging activity, inhibitory concentration 50% (IC50) (120.92â€“76.38 %), respectively. The results indicate that isolated compound CR-1 and CR-2 having IC50 76.38 and 76.94 Î¼g/ml, respectively, showed potent antioxidant activity comparable to standard ascorbic acid (IC50 43.42 Î¼g/ml).

Conclusion: This study suggests that areal part of C. reflexa have bioactive compounds for a new antimicrobial and antioxidant drug development.

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