CHROMATOGRAPHY-BASED PARTIAL PURIFICATION AND CHARACTERIZATION OF ANTIMICROBIAL COMPOUND ISOLATED FROM A TERRESTRIAL STREPTOMYCES
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i2.22501Keywords:
Terrestrial Streptomyces, Antimicrobial compound, Characterization, Liquid chromatography-mass spectrrum, Fourier-transform infrared spectroscopyAbstract
Objectives: A potent Streptomyces strain isolated from garden soil samples of Sathyabama University, Chennai, and the characterization and its antimicrobial activity was reported previously. This report deals with partial purification and characterization of the partially purified antimicrobial compound by liquid chromatography-mass spectrum (LC-MS) method.
Method: The crude extract was characterized by ultra violet-visible spectrum (UV) and Fourier transform infrared spectroscopy (FTIR) subsequently partial purification was done by thin-layer chromatography, and the bands were used for LC-MS analysis. The obtained mass spectrum was searched in NIST for species data by molecular weight. The compound was identified by comparing the mass spectrum with the known compounds mass spectrum having 98-100% identical. The biological activity of the identified compound was searched in PubChem.
Results: The UV and FTIR spectrum indicate the presence of hydroxyl group, aliphatic chain, aromatic, aliphatic amines, and ethers in the crude extract. The LC-MS spectrum revealed the presence of 12 compounds in high intensity resembling antimicrobial compounds such as imidazole, N-trifluoroacetyl, cyclohexane, 2-nitro, and stigmasterol.
Conclusion: The instrumental analysis shows the presence of 14 different compounds, and PubChem compound search shows these compounds having various biological activities including antibacterial, antifungal, anticancer, antitumor, and antidepressant. This report insists us further to evaluate the anticancer, antitumor, and antitrypanosomal activities of the partially purified compound.
Downloads
References
Sathish KS, Kokati VB. In-vitro antimicrobial activity of marine Actinobacteria against multidrug resistance Staphylococcus aureus. Asian Pac J Trop Biomed 2012;2:787-92.
Saravana Kumar P, Duraipandiyan V, Ignacimuthu S. Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7. Kaohsiung J Med Sci 2014;30:435-46.
Kumar PS, Raj JP, Duraipandiyan V, Ignacimuthu S. Antibacterial activity of some actinomycetes from Tamil Nadu, India. Asian Pac J Trop Biomed 2012;2:936-43.
Solecka J, Zajko J, Postek M, Rajnisz A. Biologically active secondary metabolites from actinomycetes. Cent Eur J Biol 2012;7:373-90.
Raja A, Prabakarana P. Actinomycetes and drug-an overview. Am J Drug Dis Dev 2011;1:75-84.
Rajeswari P, Jose PA, Amiya R, Jebakumar SR. Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity. Front Microbiol 2015;5:753.
Subramani R, Aalbersberg W. Marine actinomycetes: An ongoing source of novel bioactive metabolites. Microbiol Res 2012;10:571-80.
Singh LS, Sharma H, Talukdar NC. Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak Lake of Manipur, India. BMC Microbiol 2014;14:278.
Berdy J. Are actinomycetes exhausted as a source of secondary metabolites. Biotechnologia 1995;7:13-34.
Kishore P, Mangwani N, Dash HR, Das S. Taxonomic study of antibiotic-producing marine Actinobacteria. In: Kim SK, editor. Marine Microbiology: Bioactive Compounds and Biotechnological Applications. Weinheim: Wiley-VCH; 2013. p. 37-8.
Valan AM, Ignacimuthu S, Agastian P. Actinomycetes from western Ghats of Tamil Nadu with its antimicrobial properties. Asian Pac J Trop Biomed 2012;Suppl 2:S830-7.
Sudha S, Hemalatha R. Isolation and screening of antibiotic producing actinomycetes from garden soil of Sathyabama University, Chennai.
Asian J Pharm Clin Res 2015;8:110-4.
Umezawa H, Kondo S, Maeda K, Okami Y, Okud T, Takeda K. Index of Antibiotics from Actinomycetes. Tokyo: University of Tokyo Press; 1967.
Stead P. Isolation by preparative HPLC. In: Cannell RJ, editor. Methods in Biotechnology, Natural Products Isolation. Vol. 4. Totowa, New Jersey: Humana Press; 1998. p. 165-208.
Bystrykh LV, Fernandez-Moreno MA, Herrema JK, Malpartida F, Hopwood DA, Dijkhuizen L. Production of action rhodinrelated ‘‘blue pigments’’ by Streptomyces coelicolor A3(2). J Bacteriol 1996;178:2238-44.
Thirumalairaj J, Shanmugasundaram T, Sivasankari K, Natarajaseenivasan K, Balagurunathan R. Isolation, screening and characterization of potent marine Streptomyces Sp. Pm105 against antibiotic resistant pathogens. Asian J Pharm Clin Res 2015;8:439-43.
Slavica BI, Sandra SK, Zoran BT. UV/VIS analysis and antimicrobial activity of Streptomyces isolates. Med Biol 2005;12:44-6.
Parthasarathi S, Sathya S, Bupesh G, Samy RD, Mohan MR, Kumar GS, et al. Isolation and characterization of antimicrobial compound from marine Streptomyces hygroscopicus BDUS 49. World J Fish Mar Sci 2012;4:268-77.
Remya M, Vijayakumar R. Isolation and characterization of marine antagonistic Actinomycetes from west coast of India. Facta Univ 2008;15:13-9.
Nandhini SU, Bharathy PJ, Rekha S. Antifungal compounds from marine Streptomyces. Int J Pharm Pharm Sci 2014;7:207-9.
Saranya AV, Ravi S. Synthesis, characterization and anti-bacterial activity of pyrimidine, cyclohexanone and 1, 5-diketone derivatives of furfural chalcone. J Pharm Res 2012;5:1098-101.
Romero DH, Heredia VE, GarcÃa-Barradas O, López ME, Pavón ES. Synthesis of imidazole derivatives and their biological activities. J Chem Biochem 2014;2:45-83.
Rokade YP, Sayyed RZ. Naphthalene derivatives: A new range of antimicrobials with high therapeutic value. Rasayan J Chem 2009;2:972-80.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
