PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

Sathvika Chintalapani; Swathi MS; Mangamoori Lakshmi Narasu

doi:10.22159/ajpcr.2018.v11i1.22558

Authors

Sathvika Chintalapani Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.
Swathi MS Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.
Mangamoori Lakshmi Narasu Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i1.22558

Keywords:

Phytochemical screening, Antioxidant activity, Sesuvium portulacastrum L

Abstract

Â

Â Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).

Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2â€²-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.

Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241â‰¤ r â‰¥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722â‰¤ r â‰¥0.999) between all the pairs of antioxidant assays.

Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

Downloads

Download data is not yet available.

Author Biographies

Sathvika Chintalapani, Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.

Research Scholar

Centre for Biotechnology

Swathi MS, Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.

Research Scholar

Centre for Biotechnology

References

Ramani B, Reeck T, Debez A, Stelzer R, Huchzermeyer B, Schmidt A, et al. Aster tripolium L. and Sesuvium portulacastrum L.: Two halophytes, two strategies to survive in saline habitats. Plant Physiol Biochem 2006;44:395-408.

Lokhande VH, Nikam TD, Ghane SG, Suprasanna P. In vitro culture, plant regeneration and clonal behaviour of S. portulacastrum (L.) L.: A prospective halophyte. Physiol Mol Biol Plants 2010;16:187-93.

Rojas A, Hernandez L, Pereda-Miranda R, Mata R. Screening for antimicrobial activity of crude drug extracts and pure natural products from Mexican medicinal plants. J Ethnopharmacol 1992;35:275-83.

Slama I, Ghnaya T, SavourÃ© A, Abdelly C. Combined effects of long-term salinity and soil drying on growth, water relations, nutrient status and proline accumulation of Sesuvium portulacastrum. C R Biol 2008;331:442-51.

Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: An essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev 2014;94:329-54.

Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 2010;48:909-30.

Rajkapoor B, Burkan ZE, Kumar RS. Oxidants and human diseases: Role of antioxidant medicinal plants-A review. Pharm Online 2010;1:1117-31.

Gilgun-Sherki Y, Melamed E, Offen D. Oxidative stress induced-neurodegenerative diseases: The need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 2001;40:959-75.

Ganesh S, Vennila JJ. Phytochemical analysis of Acanthus ilicifolius and Avicennia officinalis By GC-MS. Res J Phytochem 2011;5:60-5.

Gochukwu SC, Uche AI, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian J Plant Sci Res 2013;3:10-3.

Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed 2013;3:623-7.

Kumari A, Sharma RA. Estimation of total phenol, flavonoid contents and DPPH free radical scavenging activity of Oxalis corniculata Linn. Int J Bio Pharm Res 2015;6:178-81.

Prasanth NV, Dilip C, Dev KT, Augustine L, Saraswathi R. Evaluation of in-vitro cytotoxic and antioxidant activities of Ipomoea batatas. Int J Pharm Pharm Sci 2010;2:91-2.

Guedes AC, Amaro HM, GiÃ£o MS, Malcata FX. Optimization of ABTS radical cation assay specifically for determination of antioxidant capacity of intracellular extracts of microalgae and cyanobacteria. Food Chem 2013;138:638-43.

Saumya S, Basha P. In vitro evaluation of free radical scavenging activities of Panax ginseng and Lagerstroemia speciosa: A comparative analysis. Int J Pharm Pharm Sci 2010;3:165-9.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant powerâ€: The FRAP assay. Anal Biochem 1996;239:70-6.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A Review. Int Pharm Sci 2011;1:98-106.

Kren V, MartÃnkovÃ¡ L. Glycosides in medicine: The role of glycosidic residue in biological activityâ€. Curr Med Chem 2001;8:1303-28.

Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010;15:7313-52.

Antolovich M, Prenzler PD, Patsalides E, Mcdonald S, Robards K. Methods for testing antioxidant activity. Analyst 2001;127:183-98.

Badarinath AV, Mallikarjuna K, Sudhana Chetty CM, Ramkanth S, Rajan TV, Guanaprahash K. A review on in vitro antioxidant methods: Comparisions, correlations and considerations. Int J PharmTech Res 2010;2:1276-85.

Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412-22.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.

Boligon AA. Technical evaluation of antioxidant activity. Med Chem 2014;4:517-22.

Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinia grandis L. Leaf extract. Afr J Tradit Complement Altern Med 2007;5:61-73.

MagalhÃ£es LM, Segundo MA, Reis S, Lima JL. Methodological aspects about in vitro evaluation of antioxidant properties. Anal Chim Acta 2008;613:1-9.

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal 2006;19:669-75.

Trabelsi N, Megdiche W, Ksouri R, Falleh H, Oueslati S, Bourgou S, et al. Solvent effects on phenolic contents and biological activities of the halophyte Limoniastrum monopetalum leaves. Lebenson Wiss Technol 2010;43:632-9.

Piluzza G, Bullitta S. Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the mediterranean area. Pharm Biol 2011;49:240-7.