PHYLOGENETIC CHARATERIZATION OF LISTERIA MONOCYTOGENES ISOLATED FROM DIFFERENT SOURCES IN IRAQ

Maitham Ghaly Yousif; Ataa Khalil Al-shamari

doi:10.22159/ajpcr.2018.v11i2.22633

Authors

Maitham Ghaly Yousif Department of Biology, College of Science University of Al-Qadisiyah, Iraq.
Ataa Khalil Al-shamari Department of Biology, College of Science University of Al-Qadisiyah, Iraq.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.22633

Keywords:

Listeria monocytogenes, Phylogenetic, Polymerase chain reaction, hlyA gene sequence

Abstract

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Â Objective: The main goal of the current study was to isolate and detect the phylogenetic characterization of Listeria monocytogenes isolate from clinical and non-clinical specimens.

Methods: L. monocytogenes was isolated from 353 samples including: (94) Vaginal swabs, (81) piece of placenta, (51) frozen chicken, (69) soft cheese, and (58) frozen red meat samples using the Association of Official Agricultural Chemists method. Polymerase chain reaction (PCR) was performed for the detection of virulence gene hlyA followed by DNA sequence analysis for this gene.

Results: A total of 13 isolates of L. monocytogenes were isolated from 2 (2.1%) vaginal swabs, 4 (4.9%) piece placenta, 2 (3.9%) frozen chicken, 3 (4.3%) frozen soft cheese, and 2 (3.4%) frozen red meat samples, and hlyA gene was detected in 100% of L. monocytogenes isolates.

Conclusion: All the isolates tested positive for hlyA gene, so this is important role in the study of the L. monocytogenes infection and its pathogenicity. The phylogenetic analysis showed a clear convergence in the L. monocytogenes isolates from chicken, red meat, and soft cheese 70%, while there is a marked difference in isolates of L. monocytogenes isolated from placenta and vaginal swabs.

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Author Biography

Maitham Ghaly Yousif, Department of Biology, College of Science University of Al-Qadisiyah, Iraq.

Biology department , Collage of science, University of Al-Qadisiyah, Iraq

References

Sharma G, Dang S, Gupta S, Gabrani R. Identification and molecular characterization of bacteria having antimicrobial and anti-biofilm activity. Int J Pharm Pharm Sci 2016;8:111-4.

Jeyaletchumi P, Robin T, Paie MS, Radu S, Ghazali M, Cheah F, et al. Listeria monocytogenes in raw salad vegetables sold at retail level in Malaysia. Food Control 2010;21:774-8.

Vivant AL, Garmyn D, Maron PA, Nowak V, Piveteau P. Microbial diversity and structure are drivers of the biological barrier effect against Listeria monocytogenes in soil. PloS One 2013;8:e76991.

Jennison AV, Masson JJ, Fang NX, Graham RM, Bradbury MI, Fegan N, et al. Analysis of the Listeria monocytogenes population structure among isolates from 1931 to 2015 in Australia. Front Microbiol 2017;8:603.

Schneider KR, Goodrich-Schneider R, Hubbard MA, Sampath D. Preventing foodborne illness: Listeriosis, FSHN03-6, Food Science and

Human Nutrition Department. Florida: Florida Cooperative Extension Service, IFAS, University of Florida; 2009.

Zhang L, Moosekian SR, Todd EC, Ryser ET. Growth of Listeria monocytogenes in different retail delicatessen meats during simulated home storage. J Food Prot 2012;75:896-905.

Jadhav S, Bhave M, Palombo EA. Methods used for the detection and subtyping of Listeria monocytogenes. J Microbiol Methods 2012;88:327-41.

Lamont RF, Sobel J, Mazaki-Tovi S, Kusanovic JP, Vaisbuch E, Kim SK, et al. Listeriosis in human pregnancy: A systematic review. J Perinatal Med 2011;39:227-36.

Adriao A, Vieira M, Fernandes I, Barbosa M, Sol M, Tenreiro RP, et al. Marked intra-strain variation in response of Listeria monocytogenes dairy isolates to acid or salt stress and the effect of acid or salt adaptation on adherence to abiotic surfaces. Int J Food Microbiol 2008;123:142-50.

Hernandez-Milian A, Payeras-Cifre A. What is new in listeriosis? Biomed Res Int 2014;2014:358051.

Martinez-SuÃ¡rez JV, Ortiz S, LÃ³pez-Alonso V. Potential impact of the resistance to quaternary ammonium disinfectants on the persistence of Listeria monocytogenes in food processing environments. Front Microbiol 2016;7:638.

Bhartiam V, Mehtaa A, Singha S, Jaina N, Ahirwala L, Mehta S. Bacteriocin: A novel approach for preservation of food. Int J Pharm Pharm Sci 2015;7:20-9.

Abdalla SM. Mechanisms of Biofilm Formation by Listeria monocytogenes, Doctoral Dissertation, University of Leicester; 2014.

Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Listeria monocytogenes. Ch. 2.10.14; 2004. Available from: http://www.scientific.dept@oie.int.

Hitchins AD. Detection and Enumeration of Listeria monocytogenes in Foods, Bacteriological Analytical Manual Online; 1998. Available from: http://www.cfsan.fda.gov/ebam/bam-10.html. [Last accessed on 2008 Mar 06].

Borucki MK, Call DR. Listeria monocytogenes serotype identification by PCR. J Clin Microbiol 2003;41:5537-40.

Pan Y, Breidt FJ, Kathariou S. Resistance of Listeria monocytogenes biofilms to sanitizing agents in a simulated food processing environment. Appl Environ Microbiol 2006;72:7711-7.

Mohamed Y, Reda WW, Abdel-Moein K, El-Razik KA, Barakat AM, El Fadaly HA, et al. Prevalence and phylogenetic characterization of Listeria monocytogenes isolated from processed meat marketed in Egypt. J Genetic Eng Biotechnol 2016;14:119-23.

Kamar AM, Torky HA, El-Houshy SM. Phenotypic and genotypic characterization of Listeria monocytogenes isolated from different sources. Alex J Vet Sci 2016;51:148-57.

Lotfollahi L, Pournajaf A, Irajian G, Nowrouz J. Polymerase chain reaction (PCR4)-based detection of hly and plc-A genes in Listeria monocytogenes isolated from dairy and meat products in Iran. Afr J Microbiol Res 2014;8:1098-101.

Kargar M, Ghasemi A. Role of Listeria monocytogenes hly a gene isolated from fresh cheese in human habitual abortion in Marvdasht. Arch Clin Infect Dis 2009;4:214-8.

Churchill RL, Lee H, Hall JC. Detection of Listeria monocytogenes and the toxin listeriolysin O in food. J Microbiol Methods 2006;64:141-70.

Schnupf P, Portnoy DA. Listeriolysin O: A phagosome-specific lysin. Microbes Infect 2007;9:1176-87.

Soni DK, Singh M, Singh DV, Dubey SK. Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples. BMC Microbiol 2014;14:1417-2180.