MIMOSINE - A NOVEL SUBSTANCE AND EVALUATE THE LIPID PEROXIDATION

DHANYA K G; THANGAVEL M

doi:10.22159/ajpcr.2019.v12i3.22812

Authors

DHANYA K G Department of Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.
THANGAVEL M Department of Sree Narayana Guru College, Bharathiar University, Coimbatore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i3.22812

Keywords:

Nil

Abstract

Objective: The mimosine is a novel substance extracted and purified from Mimosa pudica. The present study is to evaluate lipid peroxidation (LPO).

Methods: The sox halation is used to differentiate extracts. Mimosine is purified by gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS). The present study was conducted to evaluate the hepatoprotective activity of ethanol extract of M. pudica against alcohol-induced albino rats. This was evident from significant of M. pudica on LPO and antioxidants liver of rats.

Results: By GC–MS and LC–MS, the mimosine is purified and it shows the highest peak. The phytochemical analysis of M. pudica and the activity of the enzyme antioxidants such as superoxide dismutase is 176.83 g and peroxidase is 0.989 g, were found to be present in the plant extract of M. pudica. In rat treated with toxic dose of alcohol, a significant increase in serum and liver were observed. Simultaneous oral administration of the aqueous extract of M. pudica reduced the lactate dehydrogenase (LDH) activity to a significant extent compared to control. LDH, a cytosolic liver marker enzyme, is a regulator of many biochemical reactions in the body tissues and the fluid.

Conclusion: The result obtained in our work, existence of bioactive substance proves that it is mainly used as a traditional medicine. The phytochemical analysis indicated the presence of alkaloids, steroids, terpenoids, tannin, phenolic, saponins, proteins, and amino acids. The liver showed that the induction of alcohol causes hepatic necrosis and injury, but it is cured by M. pudica.

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References

Kuppusamy KS. Siddha maruthuvam Inc. Indian Med Homeopathy 2012;8:584.

Mudaliyar KS. Materia medica (vegetable section), Inc. Indian Med Homeopathy 2013;1:551.

Zhang ZG, Wu L, Wang JL, Yang JD, Zhang J, Zhang J, et al. Astragaloside IV prevents MPP-induced SH-SY5Y cell death via the inhibition of bax-mediated pathways and ROS production. Mol Cell Biochem 2012;364:209-16.

Suzuki K, Mizuno Y, Yoshida M. Effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-like compounds on mitochondrial respiration. Adv Neurol 1990;53:215-8.

Smeyne RJ, Jackson-Lewis V. The MPTP model of Parkinson’s disease. Brain Res Mol Brain Res 2005;134:57-66.

Kshema J, Gopinathan N, Chitra K. Mimosa pudica Linn-a shyness princess: A review of its plant movement, active constituents, uses and pharmacological activity. Int J Pharm Sci Res 2014;5:5104-18.

Ahmad H, Sehgal S, Mishra A, Gupta R. Mimosa pudica L. (Laajvanti): An overview. Pharmacogn Rev 2012;6:115-24.

Sibi PI, Delphia PG. Nootropic studies of ethanolic extract of Mimosa pudica Linn. In albino wistar rats. Am J Phytomed Clin Ther 2013;3:266-75.

Patro G, Kumar Bhattamisra S, Kumar Mohanty B. Effects of Mimosa pudica L. Leaves extract on anxiety, depression and memory. Avicenna J Phytomed 2016;6:696-710.

Lv C, Hong T, Yang Z, Zhang Y, Wang L, Dong M, et al. Effect of quercetin in the 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced mouse model of Parkinson’s disease. Evid Based Complement Alternat Med 2012;2012:928643.

Krishnan P. The scientific study of herbal wound healing therapies: Current state of play. Curr Anaesthesia Crit Care 2006;17:21-7.

Okuno Y, Nakamura-Ishizu A, Kishi K, Suda T, Kubota Y. Bone marrow-derived cells serve as proangiogenic macrophages but not endothelial cells in wound healing. Blood 2011;117:5264-72.

Macleod J, editor. Davidson’s Principle and Practice of Medicine. 13th ed. Edinburgh UK: Churchill Livingstone; 1981. p. 590-2.

Li J, Chen J, Kirsner R. Pathophysiology of acute wound healing. Clin Dermatol 2007;25:9-18.

Wahl LM, Wahl SM. Inflammation. In: Cohen IK, Diegelman RF, Lindblad WJ, editors. Wound Healing: Biochemical and Clinical Aspects. Philadelphia, PA: W.B. Saunders; 1992. p. 40-62.

Stadelmann WK, Digenis AG, Tobin GR. Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg 1998;176:26S-38.

Sabogal-Guáqueta AM, Muñoz-Manco JI, Ramírez-Pineda JR, Lamprea-Rodriguez M, Osorio E, Cardona-Gómez GP, et al. The flavonoid quercetin ameliorates Alzheimer’s disease pathology and protects cognitive and emotional function in aged triple transgenic Alzheimer’s disease model mice. Neuropharmacology 2015;93:134-45.

Siddha Pharmacopoeia of India; 1973. p. 30-1. 19. Lakshmi N, Sasikumar M, Sunita S, Manisha B, Ramesh S.Reversed-phase high-performance thin-layer chromatographic quantification of mimosine from the whole plant of Mimosa pudica. Int J Pharm Chem 2007;20:49-51.

Wu CR, Tsai CW, Chang SW, Lin CY, Huang LC, Tsai CW, et al. Carnosic acid protects against 6-hydroxydopamine-induced neurotoxicity in in vivo and in vitro model of Parkinson’s disease: Involvement of antioxidative enzymes induction. Chem Biol Interact 2015;225:40-6.

Hanna JR, Giacopelli JA. A review of wound healing and wound dressing products. J Foot Ankle Surg 1997;36:2-14.

Albritton JS. Complications of wound repair. Clin Podiatr Med Surg 1991;8:773-85.

Rosen JS, Cleary JE. Surgical management of wounds. Clin Podiatr Med Surg 1991;8:891-907.

Senthil Kumar M, Sripriya R, Vijaya Raghavan H, Sehgal PK. Wound healing potential of Cassia fistula on infected albino rat model. J Surg Res 2006;131:283-9.

Cuzzell JZ, Stotts NA. Wound care. Trial and error yields to knowledge. Am J Nurs 1990;90:53-60, 63.

LaVan FB, Hunt TK. Oxygen and wound healing. Clin Plast Surg 1990;17:463-72.