STUDY OF IN VITRO ANTI-LITHIATIC EFFECT OF IPOMOEA BATATAS (L) LEAVES AND TUBEROUS ROOTS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i2.23319Keywords:
Anti-lithiatic, Calcium oxalate stones, Crystal nucleation and aggregation, In vitro Anti-lithiatic, Ipomoea batatas, Kidney stonesAbstract
 Objective: The present study was to demonstrate the in vitro anti-lithiatic effect of Ipomoea batatas (Convolvulaceae) leaves and tuberous roots.
Methods: The obtained ethanolic extract of I. batatas leaves and tuberous roots (EIBL and EIBR) and aqueous extract of I. batatas leaves and roots (AIBL and AIBR) were used for this in vitro study. The dissolution method of calcium oxalate by titrimetry method and calcium phosphate by colorimetric method was studied. Nucleation and aggregation of calcium oxalate crystals were determined by a spectrophotometric assay.
Results: In the estimation of calcium oxalate by titrimetry method, the I. batatas leaves and roots have very significant (p<0.01) capability to dissolve calcium oxalate. Percentage dissolution of calcium oxalate crystals was found to be 37.53%, 22.74%, 39.74%, and 24.28% for EIBL, AIBL, EIBR, and AIBR, respectively. In the estimation of calcium phosphate by colorimetric method, the percentage dissolution of calcium phosphate crystals by EIBL, AIBL, EIBR, and AIBR was found to be 67.15%, 43.17%, 76.74%, and 47.96%, respectively. The I. batatas leaves and roots were significantly (p<0.01) dissolved calcium phosphate also. The results were clearly shown that I. batatas extracts significantly (p<0.01) inhibited both nucleation and aggregation of calcium oxalate crystals by concentration-dependent manner. The maximum percent inhibition of calcium oxalate nucleation by EIBL, AIBL, EIBR, and AIBR was found to be 59.09%, 50.0%, 84.09%, and 47.73%, respectively, at 1000 μg/ml. The EIBL, AIBL, EIBR, and AIBR were inhibited calcium oxalate aggregation by 63.46%, 36.54%, 84.61%, and 42.3%, respectively, at 1000 μg/ml.
Conclusions: The results clearly indicate that I. batatas leaves and tuberous roots were found to express in vitro anti-lithiatic potential.
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References
Coe F, Parks J. Pathophysiology of kidney stones and stratergies for treatment. Hosp Pract 1983;23:145-68.
Sunitha J, Asha S, Taju G. Protective effect of Spirulina on ethylene glycol induced urolithiasis in rats. Int Res J Pharm 2012;3:444-8.
Kishimoto T, Yamamoto K, Sugimoto T, Yoshihara H, Maekawa M. Side effects of extracorporeal shock-wave exposure in patients treated by extracorporeal shock-wave lithotripsy for upper urinary tract stone. Eur Urol 1986;12:308-13.
Christina AJ, Muthumani P. Phytochemical investigation and anti lithiatic activity of Abelmoschus moschatus medikus. Int J Pharm Pharm Sci 2013;5:108-13.
Pak CY. Prevention and treatment of kidney stones. Role of medical prevention. J Urol 1989;141:798-801.
Alok S, Jain SK, Verma A, Kumar M, Sabharwal M. Pathophysiology of kidney, gallbladder and urinary stones treatment with herbal and allopathic medicine: A review. Asian Pac J Trop Dis 2013;3:496-504.
Dahanukar A, Kulkarni RA, Rege NN. Pharmacology of medicinal and natural products. Indian J Pharm 2000;32:81-118.
Hue SM, Boyce AN, Somasundram C. Comparative study on the antioxidant activity of leaf extract and carotenoids extract from Ipomoea batatas var. Oren (Sweet potato) Leaves. World Acad Sci Eng Technol 2011;58:584-7.
Ojong PB, Njiti V, Guo Z, Gao M, Besong S, Barnes SL. Variation of flavonoid content among sweet potato accessions. J Am Soc Hortic Sci 2008;133:819-24.
Islam S, Yoshimoto Y, Yamakawa O. Distribution and physiological function of caffeoylquinic acid derivatives in sweet potato genotypes. J Food Sci 2003;68:111-6.
Anbuselvi S, Muthumani S. Phytochemical and antinutritional constituents of sweet potato. J Chem Pharm Res 2014;6:380-3.
Warrier PK, Nambiar VP, Ramankutty C. Arya Vaidya Sala. Indian Medicinal Plants. Vol. 3. New Delhi: Orient Longman Publication; 1995. p. 218.
Sivasankari B, Anandharaj M, Gunasekaran P. An ethnobotanical study of indigenous knowledge on medicinal plants used by the village peoples of Thoppampatti, Dindigul district, Tamilnadu, India. J Ethnopharmacol 2014;153:408-23.
Gupta S, Mukherjee M, Kumar R. Some medicinal and aromatic plants from the districts of west Bengal used in the treatment of kidney stones along with their status. Int J Pharm Res Health Sci 2015;3:661-8.
Pochapski MT, Fosquiera EC, Esmerino LA, Dos Santos EB, Farago PV, Santos FA, et al. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves’ extract from Ipomoea batatas (L.) lam. Pharmacogn Mag 2011;7:165-70.
Khandelwal KR. Practical Pharmacognosy Techniques and Experiments. 10thed. Pune: Nirali Prakashan; 2003. p. 140-57.
Phatak RS, Hendre AS. In-vitro antiurolithiatic activity of Kalanchoe pinnata extract. Int J Pharmacogn Phytochem Res 2015;7:275-9.
Khare P, Mishra VK, Arun K, Bais N, Singh R. Study on in vitro activity of Phyllanthus niruri linn. Leaves by homogenous precipitation and turbiditory method. Int J Pharm Pharm Sci 2014;6:124-7.
Bawane P, Jain M, Gawad J, Godad A, Tauro S. Evaluation of polyherbal extracts for In-Vitro antiurolithiatic activity using bioenhancer. Eur J Biomed Pharm Sci 2014;1:480-9.
Atmani F, Khan SR. Effects of an extract from Herniaria hirsuta on calcium oxalate crystallization in vitro. BJU Int 2000;85:621-5.
Saha S, Verma RJ. Inhibition of calcium oxalate crystallization in vitro by an extract of Bergenia ciliata. Arab J Urol 2013;11:187-92.
Basavaraj DR, Biyani CS, Browning AJ, Cartledge JJ. The role of urinary kidney stone inhibitors and promoters in the pathogenesis of calcium containing renal stones. EAU-EBU Update Ser 2007;5:126-36.
Consensus conference. Prevention and treatment of kidney stones. JAMA 1988;260:977-81.
Khan SR. Structure and development of calcific urinary stones. In: Bonucci E, editor. Calcification in Biological Systems. Boca Raton: CRC Press; 1992. p. 345-63.
Patel MA, Patel PK, Seth AK. Inhibition of calcium oxalate crystallization by the fruit extracts of Piper nigrum L. Pharm Online 2011;2:1169-77.
Kalpana S, Nirmaladevi R, Shrinidhi Rai T, Karthika P. Inhibition of calcium oxalate crystallization in vitro by extract of banana cultivar monthan. Int J Pharm Pharm Sci 2013;5:649-53.
Teow CC, Truong VD, McFeeters RF, Thompson RL, Pecota KV, Yencho GC. Antioxidant activities, phenolic and carotene contents of sweet potato genotypes with varying flesh colors. Food Chem 2007;103:829-38.
Antia BS, Akpan EJ, Okon PA, Umoren IU. Nutritive and anti-nutritive evaluation of sweet potatoes (Ipomoea batatas) leaves. Pak J Nutr 2006;5:166-8.
Yoshimoto M, Okuno S, Yoshinaga M, Yamakawa O, Yamaguchi M, Yamada J, et al. Antimutagenicity of sweetpotato (Ipomoea batatas) roots. Biosci Biotechnol Biochem 1999;63:537-41.
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