PHYSICOCHEMICAL PROPERTIES, PHYTOCHEMICAL SCREENING, AND ANTIHYPERGLYCEMIC ACTIVITY OF INAI (LAWSONIA INERMIS L.) LEAVES ACTIVE FRACTION ON STREPTOZOTOCININDUCED DIABETIC MICE
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i5.23504Keywords:
Antihyperglycemic, Diabetic, Inai, Streptozotocin, MiceAbstract
 Objective: This study was designed to explore the preliminary phytochemical and physicochemical analysis of Inai (Lawsonia inermis L.) leaves and further evaluation of its antihyperglycemic effect on diabetic rats from its active fraction.
Methods: The study begins with making the powder of Inai (L. inermis L.) leaves and then evaluates the physicochemical characterization. The powder of Inai leaves was fractionated and performed by the standard phytochemical screening method and streptozotocin-induced diabetic mice for evaluated the antihyperglycemic effect of Inai leaves.
Result: The physicochemical evaluation showed that the powder of Inai leaves has a good and high purity level; while the phytochemical screening showed that Inai leaves fractions have various phytoconstituents. The active fraction which was obtained from ethyl acetate fraction showed a significant reduction of the blood glucose.
Conclusion: The leaves of Inai (L. inermis L.) have the antihyperglycemic activity.
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References
World Health Organization, WHO. Diagnosis and Classification of Diabetes Mellitus and its Complication. Geneva: WHO Press; 1999. p. 4-46.
Yan Z, Liying W, Zhongsu M, Jia C, Jingbo L. Anti-diabetic, anti-oxidant and anthi-hyperlipidemic activities of flavonoids from corn silk on STZ induced diabetic mice. Molecules 2016;21:1-11.
Ristow M. Neurodegenrative disorders associated with diabeter mellitus. J Mol Med (Berl) 2014;82:510-29.
Wild S, Roglic S, Green A, Sicree R, King H. Global prevalence of diabetes. Diabetes Care 2004;27:1047-53.
Marbun N. Comparison of Blood Glucose Levels Decrease Effectiveness of Pumpkin Flesh and Seeds Toward Diabetic Mice, Thesis 2017, Faculty of Pharmacy, University of Sumatera Utara; 2017. p. 1-106.
Sikarwar MS, Patil MB, Kokate CK, Sharma S, Bhat V. Antidiabetic activity of Nerium indicum leaf extract in alloxan-induced diabetic rats. J Young Pharm 2009;1:330-5.
Rumanti RM. Characterization of Simplicia and Chromatographic Analysis of Active Extract Lotus (Nelumbo nucifera Gaertn) which Can Reduce Blood Glucose in Mice, Thesis 2017, Faculty of Pharmacy, University of Sumatera Utara; 2017. p. 1-104.
Juárez-Rojop IE, Tovilla-Zárate CA, Aguilar-DomÃnguez DE, Roa-de la Fuente LF, Lobato-GarcÃa CE, Blé-Castillo JL, et al. Phytochemical screening and hypoglycemic activity of Carica papaya leaf in streptozotocin-induced diabetic rats. Rev Bras Farmacogn 2014;24:341-7.
Lal VK, Gupta PP, Awanish P. Hypoglycemic effect of Kyllinga trceps in STZ induced diabetic rats. J Diabetes Metab 2012;3:1-3.
Chaudhary G, Goyal S, Poonia P. Lawsonia inermis L: A phytopharmacological review. Int J Pharm Sci Drug Res 2010;2:91-8.
Chikaraddy A, Maniyar Y, Mankapur B. Hypoglycemic activity of ethanolic extract of Lawsonia inermis L. in alloxan induced diabetic albino rats. Int J Pharm Biol Sci 2012;2:289-91.
Inawati I, Syamsudin S, Winarno H. Effect of Inai leaf extract (Lawsonia inermis Linn.) against decreased glucose, total cholesterol and blood triglycerides on aloksan-induced mice. J Kimia Indones 2006;1:71-7.
Zubrdiah L, Nurul D, Auerkari I. Khasiat Daun Lawsonia inermis L. Sebagai Obat Tradisional Antibakteri. Surabaya: Kongres PDGI XXIII; 2008.
Borade A, Babasahe B, Kale K, Shete R. A phytopharmacological review on Lawsonia inermis L. Int J Pharm Life Sci 2011;2:536-41.
Yadav S, Kumar A, Dora J, Kumar A. Essential perspectives of Lawsonia inermis. Int J Pharam Chem Sci 2013;2:888-96.
Sinulingga SE. Angiogenesis Effect of Ethyl Acetate Fraction Ointment of Karamun Ting Leaves on Rat Wound that Infected by Bacteria, Thesis 2017, Faculty of Pharmacy, University of Sumatera Utara; 2017. p. 1-72.
Ahmed D, Saeed R, Shakeel N, Fatima K, Arshad A. Antimicrobial activities of methanolic extract of Carissa opaca roots and its fractions and compounds isolated from the most active ethyl acetate fraction. Asian Pac J Trop Biomed 2015;5:541-5.
World Health Organization, WHO. Quality Control Methods for Medicinal Plant Materials. Geneva: WHO; 1998. p. 4-46.
Depkes RI. Indonesia Ministry of Health. Materia Medika. 6th ed. Jakarta: Ditjen POM; 1995. p. 297-307
Farnsworth NR. Biologycal and phytochemical screening of plants.
Pharm Sci 1996;55:225-76.
Harbone JB. Phtyochemistry method. ITB 1987;2(6):49.
World Health Organization, WHO. Quality Control Methods for Medicinal Plant Materials. Geneva: WHO; 1992. p. 31-3.
Kabbaoui ME, Chda A, Mejrhit N, Azdad O, Farah A, Aarab L, et al. Antidiabetic effect of Thymus satureioides aqueous extract in streptozotocin-induced diabetic rats. Int J Pharm Pharm Sci 2016;8:140-5.
Bharathi TR, Prakash HS. Comparative evaluation of antidiabetic and antioxidant potency of different extracts obtained from Memecylon species. Int J Pharm Pharm Sci 2017;9:187-91.
Tiedge M, Lenzen S. Effect of glucose refeeding and glibenclamide treatment on glucokinase and GLUT 2 gene expression in pancreatic β-cells and liver from rats. Biochem J 1995;308:925-8.
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