GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM THE LEAF EXTRACT OF ADHATODA VASICA NEES. AND ASSESSMENT OF ITS ANTIBACTERIAL ACTIVITY
Abstract
Introduction: Green synthesis of nanoparticles has been an exploring research topic in recent days due to their advanced uses in biomedical, chemical,
and related fields.
Objective: The objective of the present study was to rapidly synthesize silver nanoparticles (AgNPs) using Adhatoda vasica leaf extract. The nanoparticles
obtained have been characterized with various techniques like ultraviolet-visible spectrum, Fourier transform (FT) infrared spectrometry, scanning
electron microscopy, energy dispersive spectroscopy, X-ray diffraction, atomic force microscopy, and FT-Raman spectroscopy.
Results: These techniques showed the formation of AgNPs with an average size of 21.1-29.1 nm. Phytochemicals present in the plant were responsible
for the quick reduction of Ag+ ions to Ag0 nanoparticles. The synthesized AgNPs had the potential to mitigate the bacterial and fungal cell proliferation
particularly Staphylococcus aureus, Pseudomonas auroginosa, Bacillus thuringiensis, Escherichia coli, Klebsiella pneumoniae and fungal species
were Aspergillus niger, Aspergillus flavous, Pencillium chrysogenum, and Trichoderm harizanum. The results were compared with ciprofloxacin and
fluconazole as positive controls.
Conclusion: A. vasica being a crucial medicinal plant widely used in the treatment of cancer, the present study shows that the plant can be used as best
source for synthesis of AgNPs for further research in the cancer field.
Keywords: Adhatoda vasica, Phytochemials, Phenols, Fourier transform Raman spectroscopy, Atomic force microscopy.
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Ahmed RS, Sara M, Hamid RS, Hossein J, Ashraf-A Sadat N. Rapid synthesis of silver nanoparticles using culture supernatants of enterobacteria: A novel biological approach. Biochemistry 2007;42:919-23.
Nel A, Xia T, Mädler L, Li N. Toxic potential of materials at the nanolevel 2006;311:622-7.
De Jong WH, Borm PJ. Drug delivery and nanoparticles: Applications and hazards. Int J Nanomedicine 2008;3(2):133-49.
Sivakumar P, Sivakumar P, Anbarasu K, Padian K, Renganathan S. Synthesis of silver nanorods from food industrial waste and their application in improving the keeping quality of milk. Int Eng Chem Press 2013;52:17676-81.
Nodwory PL, Wang J, Tredget EE, Burrell RE. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm 2010;7:13.
Shrivastava S, Bera T, Roy A, Sing G, Ramachandrarao P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 2007;18:225103.
Yu H, Xu X, Chen X, Lu T, Zhang P, Jing X. Preparation and antibacterial activity of acticoat antimicrobial barrier dressing. J Burn Care Rehabil 2007;20:195-200.
Mary SC, Murugan K, Roni M, Jothi S, Suganya NA. Larvicidal potential of silver nanoparticles synthesized using Adiantum capillus verenis against anopheles stephensi. Int J Curr Trop Med Health Res 2013;1:009-0158.
Linga Rao M, Savithramma N. Antimicrobial activity of silver nanoparticles synthesized by using stem extract of Svensonia hyderobadensis (Walp.) Mold – A rare medicinal plant. Res Biotechnol 2011;3:41-7.
Venkateswalu P, Ankanna S, Prasad EE, Nagajyothi PC, Savithramma N. Green synthesis of silver nanoparticles using Shorea tumbuggaia stem bark. Int J Drug Dev Res 2010;2:720-3.
Linga Rao M, Bhumi G, Savithramma N. Green synthesis of silver nanoparticles by Allamanda cathartica L. leaf extract and evaluation for antimicrobial activity. Int J Pharm Sci Nanotechnol 2013;6:2260-12. Atal CK. Chemistry and Pharmacology of Vasicine: A New Oxytosis and Abortifacient. Jammu Tawi, India: RRL.; 1980. p. 2260-8.
Wakhloo RL, Wakhloo D, Gupta OP, Atal CK. Vasicine hydrochloride: A new drug for interruption of pregnancy. J Obstet Gynaecol 1979;29:939-40.
Doshi JJ, Patel VK, Bhatt HV. Effect of Adhatoda vasica massage in pyorrhea. Int J Crude Drug Res 1983;21:173-6.
Dhuley JN. Antitussive effect of Adhatoda vasica extract on mechanical or chemical stimulation-induced coughing in animals. J Ethnopharmacol 1996;67:361-5.
Kulakarni AA. Ray of hope for cancer patients. Proceedings of International Seminar on Holistic Management of Cancer (Ayurveda Education Series No. 67); 1998. p. 5-11.
Ramy M, Fayed SA, Mahamoud GI. Chemical compositions, antiviral and antioxidant activities of seven essential oils. J Appl Sci Res 2010;6:50-62.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall, Academic Press; 1973. p. 1-32.
Bauer AW, Kirby WM, Sherris JC, Turek M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1996;45:493-6.
Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003;10(10):813-29.
Jha AK, Prasad K, Prasad K, Kulkarni AR. Plant system: Nature’s nanofactory. Colloids Surf B Biointerfaces 2009;73(2):219-23.
Chen Z, Zhang X, Cao H, Huang Y. Chitosan-capped silver nanoparticles as a highly selective colorimetric probe for visual detection of aromatic ortho-trihydroxy phenols. Analyst 2013 21;138:2343-9.
Balaji DS, Basavaraja S, Deshpande R, Mahesh DB, Prabhakar BK, Venkataraman A. Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Colloids Surf B Biointerfaces 2009;68(1):88-92.
Ahmad A, Mukherjee P, Mandal D, Senapati S, Khan MI, Kumar R, et al. Enzyme mediated extracellular synthesis of CdS nanoparticles by the fungus, Fusarium oxysporum. J Am Chem Soc 2002;124(41):12108‑9.
Rastogi L, Arunachalam J. Sunlight based irradiation strategy for rapid green synthesis of highly stable silver nano particles using aqueous garlic (Allium sativum) extract and their antibacterial potential. Mater Chem Phys 2011;129:558-63.
Rajeshkumar S, Kannan C, Annadurai G. Synthesis and characterization of antimicrobial silver nanoparticles using marine brown seaweed Padina tetrastromatica. Drug Invent Today 2012;4:511-3.
Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 2000;52(4):662‑8.
Sivakumar V, Nagaraja BM, Shashikala V, Padmasri AH, Madhavendra SS, Raju BD, et al. Highly efficient Ag/C catalyst prepared by electro-chemical deposition method in controlling microorganisms in water. Mol Catal A Chem 2004;223:313-9.
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