CARDIOPROTECTIVE EFFECT OF VITAMIN E AGAINST MYOCARDIAL INFARCTION INDUCED BY ISOPRENALINE IN ALBINO RATS

Abdelbaset Taher Abdelhalim; Nuruddin Mohammed Nur; Sherif Mansour; Abdelnasser Ibrahim

doi:10.22159/ajpcr.2018.v11i6.24999

Authors

Abdelbaset Taher Abdelhalim Department of Pharmacology, Faculty of Medicine, Alazhar University, Cairo, Egypt.
Nuruddin Mohammed Nur Department of Biochemistry, Universiti Islam Antarabangsa Sultan Abdul Halim Muâ€™adzam Shah 09300 Kuala Ketil Kedh, Darul Aman, Malaysia.
Sherif Mansour Department of Pharmacology, Faculty of Medicine, Alazhar University, Assuit, Egypt.
Abdelnasser Ibrahim Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i6.24999

Keywords:

Vitamin E, Cardioprotective, Isoprenaline, Myocardial infraction

Abstract

Objective: Vitamin E is an antioxidant which can help in the prevention of cardiovascular disease. The objective of this study was to estimate the effects of Vitamin E on cardiac marker enzymes in isoprenaline (ISO)-induced myocardial infracted rats.

Methods: Adult male albino rats were divided into three groups. The first group was the control negative group. The second group was the control positive group that was subcutaneously injected with ISO (100 mg/kg). The third group was pretreated with Vitamin E (100 mg/kg) once daily for 30 days, then subcutaneously injected with ISO at an interval of 24 h for 2 days. Aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), cardiac troponin-I (CTn-I), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and histopathological examinations were measured. Comparison between groups was achieved by one-way analysis of variance (ANOVA) test through SPSS software.

Results: The levels of AST, ALT, LDH, creatine kinase (CK), and CTn-I significantly decreased in pretreated group with Vitamin E compared to its increasing in the control positive group. The level of GSH and SOD markedly increased in pretreated group with Vitamin E compared to its decreasing in the control positive group, while the level of MDA significantly decreased in pretreated group with Vitamin E compared to its increasing in the control positive group. ISO + Vitamin E rats group reflected a cardioprotective role of Vitamin E in myocardial infarcted rats.

Conclusion: Pretreatment with Vitamin E can protect the myocardial membranes against ISO-induced oxidative stress in rats and can be used for routine clinical and epidemiological purposes.

Downloads

Download data is not yet available.

References

De Bono DP, Boon NA. Diseases of the cardiovascular system. In: Edwards CR, Boucheir IA, editors. Davidsonâ€™s Principles and Practice of Medicine. Hong Kong: Churchill Livingstone; 1992. p. 249-340.

Upaganlawar A, Gandhi H, Balaraman R. Isoproterenol induced myocardial infarction: Protective role of natural products. J Pharmacol Toxicol 2011;6:1-17.

Dhalla NS, Dixon IM, Suzuki S, Kaneko M, Kobayashi A, Beamish RE. Changes in adrenergic receptors during the development of heart failure. Mol Cell Biochem 1992;114:91-5.

Mathers C. The Global Burden of Disease: 2004 Update. Geneva: World Health Organization; 2008.

Gupta R, Joshi P, Mohan V, Reddy KS, Yusuf S. Epidemiology and causation of coronary heart disease and stroke in india. Heart 2008;94:16-26.

La Vecchia C, Decarli A, Pagano R. Vegetable consumption and risk of chronic disease. Epidemiology 1998;9:208-10.

Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. 3rd ed. Oxford: Oxford University Press; 1999.

Wiseman SA, Balentine DA, Frei B. Antioxidants in tea. Crit Rev Food Sci Nutr 1997;37:705-18.

Vajragupta O, Boonchoong P, Berliner LJ. Manganese complexes of curcumin analogues: Evaluation of hydroxyl radical scavenging ability, superoxide dismutase activity and stability towards hydrolysis. Free Radic Res 2004;38:303-14.

Geier DA, Kern JK, Garver CR, Adams JB, Audhya T, Nataf R, et al. Biomarkers of environmental toxicity and susceptibility in autism. J Neurol Sci 2009;280:101-8.

LÃ¼ JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: Experimental approaches and model systems. J Cell Mol Med 2010;14:840-60.

DeFeudis FV, Papadopoulos V, Drieu K. Ginkgo biloba extracts and cancer: A research area in its infancy. Fundam Clin Pharmacol 2003;17:405-17.

Rababah TM, Hettiarachchy NS, Horax R. Total phenolics and antioxidant activities of fenugreek, green tea, black tea, grape seed, ginger, rosemary, gotu kola, and ginkgo extracts, vitamin E, and tert-butylhydroquinone. J Agric Food Chem 2004;52:5183-6.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.

Heart Outcomes Prevention Evaluation Study Investigators, Yusuf S, Dagenais G, Pogue J, Bosch J, Sleight P, et al. Vitamin E supplementation and cardiovascular events in high-risk patients.N Engl J Med 2000;342:154-60.

Institute of Medicine. Food and Nutrition Board. Dietary Reference Intakes: Vitamin C, Vitamin E, Selenium, and Carotenoids. Washington, DC: National Academy Press; 2000.

Bursell SE, King GL. Can protein kinase C inhibition and vitamin E prevent the development of diabetic vascular complications? Diabetes Res Clin Pract 1999;45:169-82.

Siddiqui MA, Siddiqui HH, Ahmad U, Badruddeen, Khan AA. Isoprenaline: A tool for inducing myocardial infarction in experimental animal. Int J Res Rev Pharm Appl Sci 2016;6:1318-26.

Arya DS, Bansal P, Ojha SK, Nandave M, Mohanty I, Gupta SK, et al. Pyruvate provides cardioprotection in the experimental model of myocardial ischemic reperfusion injury. Life Sci 2006;79:38-44.

Eman SA. Cardioprotective efficacy of taurine on lipid-metabolism of isoproterenol-induced myocardial infarction. Int J Pharm Pharm Sci 2016;8:135-41.

Chikku AM, Rajamohan T. Coconut haustorium maintains cardiac integrity and alleviates oxidative stress in rats subjected to isoproterenol-induced myocardial infarction. Indian J Pharm Sci 2012;74:397-402.

Upaganlawar A, Gandhi H, Balaraman R. Effect of vitamin E alone and in combination with lycopene on biochemical and histopathological alterations in isoproterenol-induced myocardial infarction in rats. J Pharmacol Pharmacother 2010;1:24-31.

Priscilla DH, Prince P. Cardioprotictive effect of gallic acid in experimentally induced myocardial infarction in rat. Chem Biol Interact 2009;179:118-24.

Kumaran KS, Prince PS. Caffeic acid protects rat heart mitochondria against isoproterenol-induced oxidative damage. Cell Stress Chaperones 2010;15:791-806.

Lobo Filho HG, Ferreira NL, Sousa RB, Carvalho ER, Lobo PL, Lobo Filho JG, et al. Experimental model of myocardial infarction induced by isoproterenol in rats. Rev Bras Cir Cardiovasc 2011;26:469-76.

Upaganlawar A, Gandhi H, Balaraman R. Effect of vitamin E alone and in combination with lycopene on biochemical and histopathological alterations in isoproterenol-induced myocardial infarction in rats. J Pharmacol Pharmacother 2010;1:24-31.

Rahman MD, Islam SN. Effect of serum antioxidants (vitamin E, C and A) in lung cancer patients. Int J Pharm Pharm Sci 2014;6:578-80.