EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL.
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i10.25008Keywords:
Wedelia chinensis, In vitro antioxidant activity, Inhibition concentration50, Methanolic leaf extractAbstract
Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.
Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.
Results: Inhibition concentration50 values observed for DPPH radical scavenging, superoxide radical scavenging, and hydroxyl radical scavenging activities were determined to be 62.5, 769.23, and 617.28 μg/ml, respectively and that of FRAP scavenging, metal chelating, and total antioxidant activities were found to be 343 μmol Fe (II)/g, 79.56 mg EDTA/g extract, and 398.17 mg ascorbic acid eq/g extract, respectively.
Conclusion: The results clearly indicate that methanolic leaf extract of the study species W. chinensis is effective in scavenging free radicals and has the potential to be a powerful antioxidant.
Downloads
References
MartÃnez-Cayuela M. Oxygen free radicals and human disease. Biochimie 1995;77:147-61.
Ansari KN. The free radicals – the hidden culprits – an update. Indian J Med Sci 1997;51:319-36.
Ozcan MM, Evel O, Herken EE. Antioxidant activity, phenolic content and peroxide value of essential oil and extracts of some medicinal and aromatic plants used as condiments and herbal teas in Turkey. J Med Food 2009;12:198-202.
Habila JD, Bello IA, Dzikwi AA, Musa H, Abubakar N. Total phenolics and antioxidant activity of Tridax procumbens Linn. A J Pharm Pharmacol 2010;4:123-6.
Halliwell B. Antioxidants and human disease: A general introduction. Nutr Rev 1997;55:S44-9.
Steer P, Millgård J, Sarabi DM, Basu S, Vessby B, Kahan T, et al. Cardiac and vascular structure and function are related to lipid peroxidation and metabolism. Lipids 2002;37:231-6.
Ghosal S, Tripathi VK, Chauhan S. Active constituents of Emblica officinalis: Part I: The chemistry and antioxidant effects of two new hydrolysable tannins, emblicanin A and B. Indian J Chem 1996;35:941 8.
Yam MF, Basir R, Asmawi MZ, Ahmad MR, Akowuah GA. Antioxidant and hepatoprotective activities of Elephantopus tomentosus ethanol extract. Pharm Biol 2008;46:199-206.
Abdollahi M, Larijani B, Rahimi R, Salari P. Role of oxidative stress in osteoporosis. Therapy 2005;2:787-96.
Liu S, Manson JE, Lee IM, Cole SR, Hennekens CH, Willett WC, et al. Fruit and vegetables intake and risk of cardiovascular disease: The women’s study. Am J Clin Nutr 2000;72:922-8.
Guorong L, Shaohui L, Peng J, Huitong L, Boyi L, Wanhong X, et al. Cerebrovascular blood flow dynamic changes in fetuses with congenital heart disease. Fetal Diagn Ther 2009;25:167-72.
Chopra RN. Glossary of Indian Medicinal Plants. New Delhi: Council of Scientific and Industrial Research; 1965. p. 258.
Matthew KM. The flora of the Tamil Nadu Carnatic. Tiruchirapalli: The Rapinat Herbarium, St. Joseph’s College; 1983. p. 2154.
Vaghasiya Y, Nair R, Baluja S, Chanda S. Antibacterial and preliminary phytochemical analysis of Eucalyptus citriodora Hk leaf. Nat Prod Rad 2008;22:754-62.
Aiyelaagbe OO, Osamudiamen PM. Phytochemical screening for active compounds in Mangifera indica leaves from Ibadan, Oyo State. Plant Sci Res 2009;2:11-3.
Yilmaz M, Turk AO, Tay T, Kivanc M. The antimicrobial activity of extract of the lichen Cladonia foliaceaandits (-) Usnic acid, atranorin and fumarprotocetraic acid constituents. Z Naturforsch 2004;59:249 54.
Brand-Williams W, Cuevelier ME, Berset C. Use of a free radical method to valuate antioxidant activity. Lebensm Wiss u-Technol 1995;28:25-30.
Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971;44:276-87.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power†the FRAP assay. Anal Biochem 1996;239:70-6.
Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry 1991;20:6006-12.
Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivates acetoaminophen, salicylate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161-9.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of Vitamin E1. Anal Biochem 1999;269:337-41.
Elmastas M, Gulcin I, Isildak O, Kufrevioglu OI, Ibaoglu K, Aboul- Enein HY. Radical scavenging activity and antioxidant capacity of Bay leaf extracts. J Iran Chem Soc 2006;3:258-66.
Tianpech N, Swatsitang P, Tanpanich S. Antioxidant capacity and nutritional values of Pak-Wanpa (Melientha suavis Pierre.). KKU Sci J 2008;38:75-82.
Khanamn S, Shivaprasad HN, Kshama D. In vitro antioxidant screening models: A review. Indian J Pharm Educ 2004;38:180-3.
Suganya T, Siriporn O, Sombat C. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem 2007;103:381-8.
Saha MR, Hasan SM, Akter R, Hossain MM, Alam MS, Alam MA, et al. In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi Linn. Bangl J Vet Med 2008;6:197-202.
Sujata RV, Rathod VS, Yesane DP. Screening of three wild edible fruits for their antioxidant potential. Curr Bot 2011;2:48-52.
Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Fazelian M, Eslami B. In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacog Mag 2009a;4:123 7.
Aboul-Enein AM, El Baz FK, El-Baroty GS, Youssef AM, Abd El Baky HH. Antioxidant activity of algal extracts on lipid peroxidation. J Med Sci 2003;3:87-98.
Shahidi F, Naczk M. Phenolics in Food and Nutraceuticals. Boca Raton, FL: CRC Press; 2004.
Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem 2007;103:381-8.
RodrÃguez J, Olea-Azar C, Cavieres C, Norambuena E, Delgado- Castro T, Soto-Delgado J, et al. Antioxidant properties and free radical-scavenging reactivity of a family of hydroxynaphthalenones and dihydroxyanthracenones. Bioorg Med Chem 2007;15:7058-65.
Meena AK, Rao MM, Kaur K, Panda P. Comparative evaluation of standardization parameters between Wedelia genus species. Int J Pharm Sci Res 2010;1:207-10.
Oktay M, Gulcin I, Vioglu OI. Determination of in vitro antioxidant activity of fennel Foeniculum vulgare seed extracts. LWT 2003;36:263 71.
Shon MY, Kim TH, Sung NJ. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 2003;82:593-7.
Lee YR, Woo KS, Kim KJ, Son JR, Jeong HS. Antioxidant activities of ethanol extracts from germinated specialty rough rice. Food Sci Biotechnol 2007;16:765-70.
Katalinic V, Milos M, Jukic M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 2006;94:550-7.
Tepe B, Sokmen M, Akpulat HA, Sokmen A. In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey. Food Chem 2005;90:685-9.
Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD, et al. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res 2007;21:615-21.
Karamenderes C, Konyalioglu S, Khan S, Khan IA. Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. Species. Phytother Res 2007;21:488-91.
Erasto P, Grierson DS, Afolayan AJ. Evaluation of antioxidant activity and the fatty acid profile of the leaves of Vernonia amygdalina growing in South Africa. Food Chem 2007;104:636-42.
Loizzo MR, Said A, Tundis R, Hawas UW, Rashed K, Menichini F, et al. Antioxidant and antiproliferative activity of Diospyros lotus L. Extract and isolated compounds. Plant Foods Hum Nutr 2009;64:264 70.
Stanojević L, Stanković M, Nikolić V, Nikolić L, Ristić D, Canadanovic-Brunet J, et al. Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. Extracts. Sensors (Basel) 2009;9:5702-14.
Pukalskas A, Venskutonis PR, Dijkgraaf I, van Beek TA. Isolation, identification and activity of natural antioxidants from costmary (Chrysanthemum balsamita) cultivated in lithuania. Food Chem 2010;122:804-811.
Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Screening for in vitro antioxidant properties and fatty acid profiles of five Centaurea L. species from turkey flora. Food Chem Toxicol 2011;49:2914-20.
Suzgec-Selcuk S, Birteksoz AS. Flavonoids of Helichrysum chasmolycicum and its antioxidant and antimicrobial activities. South Afr J Bot 2011;77:170-4.
Ravisankar N, Sivaraj C, Seeni S, Joseph J, Raaman N. Antioxidant activities and phytochemical analysis of methanol extract of leaves of Hypericum hookerianum. Int J Pharm Pharm Sci 2014;6:456-60.
Singh S, Garg V, Yadav D, Beg MN, Sharma N. In vitro antioxidative and antibacterial activities of various parts of Stevia rebaudiana (Bertoni). Int J Pharm Pharm Sci 2012;4:468-73.
Duan X, Wu G, Jiang Y. Evaluation of antioxidant properties of phenolics from litchi fruit in relation to pericarp browning prevention. Molecules 2007;12:759-71.
Wang T, Jonsdottir R, Olafsdottir G. Total phenolic compounds, radical scavenging and metal chelation of extracts from Icelandic seaweeds. Food Chem 2009a;116:240-8.
Gupta AD, Pundeer V, Bande G, Dhar S, Ranganath IR, Kumari GS. Evaluation of antioxidant activity of four folk ant diabetic medicinal plants of India. Pharmacol Online 2009a;1:200-8.
Al-Laith AA. Antioxidant components and antioxidant/antiradical activities of desert truffle (Tirmania nivea) from various Middle Eastern origins. J Food Composit Anal 2010;23:15-22.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
