EFFECT OF HEXANE FRACTION FROM PAPAYA (CARICA PAPAYA L.) MALE FLOWER ON CELL CYCLE OF COLON ADENOCARCINOMA (WIDR) CELL AND ITS COMBINATION INDEX WITH DOXORUBICIN

Yusnita Y; Masfria M; Rosidah R; Iksen I

doi:10.22159/ajpcr.2018.v11i7.25023

Authors

Yusnita Y Department of Pharmacology, Faculty of Pharmacy, Universita Sumatera Utara, Medan, Indonesia.
Masfria M Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Rosidah R Department of Pharmacology, Faculty of Pharmacy, Universita Sumatera Utara, Medan, Indonesia.
Iksen I Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i7.25023

Keywords:

Papaya male flower, Doxorubicin, Combination index, Cell cycle, WiDr

Abstract

Objective: This study aimed to evaluate the effects of papaya (Carica papaya L.) male flower hexane fraction (PHF) on cell cycle of colon adenocarcinoma (WiDr) cell and its combination index (CI) with doxorubicin.

Methods: Flow cytometer and the CI were used to show the PHF on cell cycle of colon adenocarcinoma (WiDr) cell and to calculate the synergism potential, respectively.

Result: The result showed that the PHF giving inhibition on cell cycle of colon adenocarcinoma (WiDr) cell in G0-G1, S, G2-M phase. Furthermore, the combination of PHF with doxorubicin in colon adenocarcinoma (WiDr) cell gave a strong synergistic effect with optimal concentration of 8 Î¼g/mlâ€“50 nM (PHF-Doxorubicin) with the IC score lower than 1.

Conclusion: This study provides evidence that PHF could be a new potential co-chemotherapeutic agent with doxorubicin on colon adenocarcinoma cell.

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Author Biographies

Yusnita Y, Department of Pharmacology, Faculty of Pharmacy, Universita Sumatera Utara, Medan, Indonesia.

Department of Pharmacology

Masfria M, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

Department of Pharmaceutical Chemistry

Rosidah R, Department of Pharmacology, Faculty of Pharmacy, Universita Sumatera Utara, Medan, Indonesia.

Department of Pharmacology

Iksen I, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

Department of Pharmaceutical Chemistry

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