PREVALENCE AND MOLECULAR CHARACTERIZATION OF blaCTX-M-15-PRODUCING PATHOGENIC GRAM-NEGATIVE BACTERIA FROM VARIOUS CLINICAL SAMPLES

Sindhuja S; Sureshkumar Bt; Janaki S; Thenmozhi S

doi:10.22159/ajpcr.2018.v11i10.25111

Authors

Sindhuja S Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (A), Elayampalayam - 637 205, Tiruchengode, Namakkal District, Tamil Nadu, India.
Sureshkumar Bt Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (A), Elayampalayam - 637 205, Tiruchengode, Namakkal District, Tamil Nadu, India.
Janaki S Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (A), Elayampalayam - 637 205, Tiruchengode, Namakkal District, Tamil Nadu, India.
Thenmozhi S Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (A), Elayampalayam - 637 205, Tiruchengode, Namakkal District, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i10.25111

Keywords:

CTX-M-15 gene, Clinical samples, Nil, Escherichia coli, Multidrug resistance

Abstract

Objective: The objective of this study was to describe the prevalence and molecular characterization of blaCTX-M-15-producing pathogenic Gram-negative bacteria from various clinical samples isolated from clinically suspected patients.

Methods: In this study, clinical samples of urine, stool, sputum, and pus were collected from 244 patients with nosocomial infections. The phenotypic identification of extended-spectrum Î²-lactamases (ESBL) was confirmed by double-disk synergy test and combined disk diffusion test. In vitro, the susceptibility pattern of antimicrobial agents against pathogenic isolates was performed by Kirbyâ€“Bauer disk diffusion method. The identification of blaCTX-M-15-producing Escherichia coli was assessed by polymerase chain reaction method.

Results: The frequency of ESBL-producing pathogenic bacteria from screened was 6 (46.15%). In vitro, susceptibility to pathogenic bacteria showed that the majority of isolates were highly susceptible to amoxicillin-clavulanic acid (97.87%), ofloxacin (93.33%), and Pseudomonas aeruginosa showed 100% sensitive to ceftazidime, cefotaxime, cefixime, cefoperazone, and meropenem (92.30%). The rates of resistance to other antibiotics varied from <26.66%. Among six tested isolates, only one E. coli isolates showed blaCTX-M-15 gene.

Conclusion: Due to the increase of E. coli with multiple ESBL genes, continuous surveillance should be needed in clinical field to use of appropriate antibiotics and the control of infections.

Downloads

Download data is not yet available.

Author Biography

Sindhuja S, Department of Microbiology, Vivekanandha College of Arts and Sciences for Women (A), Elayampalayam - 637 205, Tiruchengode, Namakkal District, Tamil Nadu, India.

Microbiology, research scholar

References

Kenneth S. Extended-spectrum-Î²-lactamase, AmpC, and Carbapenemase. J Clin Microbiol 2010; 48: 1019-102.

Essack SY. The development of Î²-lactam antibiotics in response to the evolution of Î²-lactamases. Pharmaceut Res 2001; 18: 1391-9.

Oberoi L, Singh N, Sharma P, Aggarwal A. ESBL, MBL and AmpC Î² Lactamases Prodical centerucing Superbugs - Havoc in the Intensive Care Units of Punjab India. J Clin Diagn Res 2013; 7: 70-73.

Bonnet R. Growing group of extended spectrum - Lactamases the CTX-M enzymes. Antibiocrob Agents Chemother 2004; 48(1): 1-14.

Abhilash KP, Veeraraghavan B, Abraham OC. Epidemiology and outcome of bacteremia caused by extended spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. in a tertiary care teaching hospital in south India. J Assoc Physicians India 2010; 58(Suppl): 13-7.

Olson AB, Silverman M, Boyd DA, McGeer A, Willey BM, Pong-Porter V. Identification of a progenitor of the CTX-M-9 group of extended-spectrum beta-lactamases from Kluyvera georgiana isolated in Guyana. Antimicrob Agents Chemother 2005; 49: 2112-5.

Parja SC. Text Book of Microbiology. Elsevier 2009; 326-329.

Wayne PA. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing: Seventeenth informational supplement. M100-S17. CLSI; 2007.

Fennel J, Vellinga A, Hanahoe B, Morris D, Boyle F, Higgins F, Lyins M, Connell K, Keudy D, Cormican, M. Increasing prevalence of ESBL production among Irish clinical Enterobacteriaceae from 2004 to 2008: An observational study. BMC Infectious Diseases 2012; 12: 1-8.

Wayne PA. Clinical and Laboratory Standard Institute (CLSI). Performance standards for antimicrobial susceptibility testing, Twenty- First informational supplement M100-S20. Clinical and laboratory standards Institute 2010; 30: 46.

Jarlier V, Nicolas MH, Fournier G, Philippon A. Extended spectrum beta lactamases conferring transferable resistance to newer beta lactam agents in Enterobacteriaceae: Hospital prevalence and susceptibility patterns. Rev Infect Dis 1988; 10: 867-878.

Ahmed OI, Hady SA, Ahmed TM, Ahmed IZ. Detection of bla SHV and bla CTX-M genes in ESBL producing Klebsiella pneumoniae isolated from Egyptian patients with suspected nosocomial infections. Egypt J Med Hum Genet 2013; 14(3): 277-83.

Abdi S, Ranjbar R, Vala MH, Jonaidi N, Bejestany OB, Bejestany FB. Frequency of blaTEM, blaSHV, blaCTX-M, and qnrA among Escherichia coli isolated from urinary tract infection. Arch Clin Infec Dis 2014; 9(1): 18690.

Gagliotti C, Balode A, Baquero F, Degener J, Grundmann HG, Ur D. Escherichia coli and Staphylococcus aureus: Bad news and good news from the European Antimicrobial Resistance Surveillance Network (EARS-Net, formerly EARSS), 2002 to 2009. Euro Surveill 2011; 16(11): 19819.

Sana T, Rami K, Racha B, Fouad D, Marcel A, Hassan M. Detection of genes TEM, OXA, SHV and CTX-M in 73 clinical isolates of Escherichia coli producers of extended spectrum beta-lactamases and determination of their susceptibility to antibiotics. Int Arab J Antimicrob Agents 2011; 1: 1-6.

Molton JS, Tambyah PA, Ang BS, Ling ML, Fisher DA.The global spread of healthcare-associated multidrug-resistant bacteria: A perspective from Asia. Clin Infect Dis 2013; 56(9): 1310-8.

Coque TM, Baquero F, Canton R. Increasing prevalence of ESBL producing Enterobacteriaceae in Europe. Euro Surveill 2008; 13(47): 19044.

Mshana SE, Imirzalioglu C, Hossain H, Hain T, Domann E, Chakraborty T. Conjugative IncFI plasmids carrying CTX-M-15among Escherichia coli ESBL producing isolates at a university hospital in Germany. BMC Infect Dis 2009; 9: 97.

Barguigua A, Otmani F, Talmi M, Bourjilat F, Haouzane F, Zerouali K. Characterization of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco. J Med Microbiol 2011; 60(9): 1344-52.

Shah AA, Hasan F, Ahmed S, Hameed A. Extended spectrum beta-lactamase in Enterobacteriaceae: Related to age and gender. New Microbiol 2002; 25: 363-6.

Sandhiya R, Lakshmi Priya R, Esthermary S. Antibiotic susceptibility pattern and ESBL prevalence in Escherichia coli isolates from pus samples in a tertiary care hospital. Int J Pharm Biol Sci 2015:7(3): 263-264.

Divyashanthi CM, Adithiyakumar S, Bharathi N. Study Of Prevalence And Antimicrobial Susceptibility Pattern Of Bacterial Isolates In A Tertiary Care Hospital. Int J Pharm Pharm Sci 2015; 7(1): 185-190.