ANTI-MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV AND IRON CHELATION ACTIVITY OF SAPPAN WOOD EXTRACT (CAESALPINIA SAPPAN L.) IN VITRO

Ratu Safitri; Ida Indrawati; Mas Rizky A A Syamsunarno; Mohammad Ghozali; Basri A Gani; Ramdan Panigoro

doi:10.22159/ajpcr.2018.v11i6.25303

Authors

Ratu Safitri Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia.
Ida Indrawati Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia.
Mas Rizky A A Syamsunarno Department of Biology, Study Program of Biotechnology, Postgraduate Study, Universitas Padjadjaran, Padjadjaran, Bandung, Indonesia.
Mohammad Ghozali Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
Basri A Gani Department of Oral Biology, Dentistry Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Ramdan Panigoro Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i6.25303

Keywords:

Iron chelation, Mycobacterium tuberculosis, Caesalpinia sappan L

Abstract

Objective: The objective of this study is to determine anti-Mycobacterium tuberculosis (MTB) strain H37Rv and iron chelation activities of sappan wood extract (SWE).

Methods: The evaluation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by proportion methods. Whereas the iron chelate in Lowensteinâ€“Jensen (LJ) medium as the indicator of Mycobacterium growth and SWE effect.

Results: The SWE has bacteriocidal to MTB of 10âˆ’3 and 10âˆ’5 dilutions in of all concentrations (250, 500, 750, 1000, 2000, 4000, 8000, and 16000 part per millions [ppm]) also bacteriostatic in concentration 50 and 100 ppm.

Conclusion: The SWE at 100 ppm could inhibit 87% of the MTB in 10âˆ’3 and 10âˆ’5 dilutions, respectively, also to reduce to growth the colony of MTB, and has chelating effects of iron expression of LJ medium and MTB.

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Author Biography

Ratu Safitri, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia.

Oral Biology Department

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