OPTIMIZATION, ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES LAVENDULOCOLOR VHB-9

Hima Bindu Bssn; Rajesh Kumar Munaganti; Vijayalakshmi Muvva; Krishna Naragani; Mani Deepa Indupalli

doi:10.22159/ajpcr.2018.v11i8.25345

Authors

Hima Bindu Bssn Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
Rajesh Kumar Munaganti Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
Vijayalakshmi Muvva Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
Krishna Naragani Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
Mani Deepa Indupalli Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i8.25345

Keywords:

Granite mine, Streptomyces lavendulocolor, Optimization, Spectroscopy, Gas chromatography-mass spectrometry analysis, Biological assay

Abstract

Objectives: Optimization, isolation, and characterization of bioactive compounds from Streptomyces lavendulocolor VHB-9 isolated from granite mines of Mudigonda village of Khammam district of Telangana state.

Methods: The potent strain was identified as S. lavendulocolor VHB-9 by polyphasic taxonomy. The influence of culture conditions on growth and bioactive compounds production was investigated. Purification of bioactive compounds was done using column chromatography. The structures of the compounds were elucidated on the basis of spectroscopic analysis including Fourier transform infrared, electron spray ionization mass spectrophotometry,1H nuclear magnetic resonance (NMR), and13C NMR. The antimicrobial activity of the compounds produced by the strain was tested against both Gram-positive and Gram-negative bacteria and fungi in terms of minimum inhibitory concentration.

Results: Isolation and identification of two compounds, namely (2R, 3R)-2, 3-Butanediol (B1A), and nonadecanoic acid (B1B). Fraction B4 was isolated partially purified fraction and identified by the gas chromatography-mass spectrometry analysis. B1B compound exhibited the highest activity against Bacillus megaterium, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans when compared to B1A and B4 compounds.

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