ENHANCEMENT OF IN VITRO ANTIOXIDANT POTENTIAL OF TERMINALIA CHEBULA BY VARIOUS FRUIT EXTRACTS AND OPTIMIZATION OF CONCENTRATION BY RESPONSE SURFACE METHODOLOGY
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i7.25786Keywords:
Terminalia chebula, Response surface methodology, Central composite design, 2, 2-diphenyl-1-picrylhydrazyl, Antioxidant potentialAbstract
Objective: The main objective of this study was to determine the enhancement of in vitro antioxidant potential of fruits of Terminalia chebula (TC) when used in combination with fruit extracts of Phyllanthus emblica, Ananas comosus, and Punica granatum. Hydroxyl (OH) radical scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were used to analyze the antioxidant potential.
Method: Formulations of different combinatorial concentrations of fruits to prepare a mixture were achieved with central composite design through response surface methodology. Screening of 300 different combinations of various concentrations was done through hydroxyl radical scavenging assay followed by statistical analysis of data. Further validation of results was done by measuring the antioxidant potential of most bioactive extracts by DPPH method.
Results: Screening of 300 samples of different combinations for antioxidant potential revealed the samples with highest percentage inhibition in aqueous (85.2%), ethanolic (92.9%), and aqueous-ethanolic (84.21%) extracts. Data were subjected to analysis of variance and generated a three-dimensional response surface plot for highest activity. Further subjecting these extracts to DPPH assay revealed a significant enhancement in the antioxidant potential of ethanolic extract of TC when used in mixture with other plants.
Conclusion: Antioxidant activity of TC was enhanced when used in combination with other fruits extracts. These synergistic studies generating valuable interactions between various phytochemicals could lead to a momentous increase in other associated activities to fight against diseases such as cancer and cardiovascular disorders. Further research on isolation of bioactive compounds in the mixture and their potential to fight various types of cancer could lead to a significant augmentation in the activity of natural compounds.
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References
Rabe T, Staden JV. Antibacterial activity of South African plants used for medicinal purposes. J Ethnopharmacol 1997;56:81-7.
Sajeesha S, Nishat A, Tripathi YC. Ethnomedicinal, phytochemical and pharmacological aspects of Flacourtia jangomas: A review. Int J Pharm Pharm Sci 2018;10:9-15.
Lie FS, Jieh HT, Je HC, Chih YC, Chiu PL. Antioxidant properties of extracts from medicinal plants popularly used in Taiwan. Int J Appl Sci Eng 2005;3:195-202.
Oviasogie PO, Okoro D, Ndiokwere CL. Determination of total phenolic amounts of some edible fruits and vegetables. Afr J Biotechnol 2009;8:2819-20.
Vidhan J, Ara DM, John RP. Anthocyanins and polyphenol oxidase from dried arils of pomegranate (Punica granatum L.). J Food Chem 2010;118:11-6.
Marja PK, Anu IH, Heikki JV, Jussi PR, Kalevi P, Tytti SK, et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999;47:3954-62.
Namjooyan F, Azemi ME, Rahmanian VR. Investigation of antioxidant activity and total phenolic content of various fractions of aerial parts of Pimpinella barbata (DC) bioss. Jundishapur. J Nat Pharm Prod 2010;5:1-5.
Vinay RP, Prakash RP, Sushil SK. Antioxidant activity of some selected medicinal plants in western region of India. Adv Boil Res 2010;4:23-6.
Sun J, Chu YF, Wu X, Liu RH. Antioxidant and antiproliferative activities of fruits. J Agric Food Chem 2010;50:7449-54.
Chu YF, Sun J, Wu X, Liu RH. Antioxidant and antiproliferative activities of vegetables. J Agric Food Chem 2002;50:6910-6.
Eberhardt MV, Lee CY, Liu RH. Antioxidant activity of fresh apples. Nature 2002;405:903-4.
Delanty N, Dichter MA. Antioxidant therapy in neurologic disease. Arch Neurol 2000;57:1265.
Naik GH, Priyadarsini KI, Naik DB, Gangabhagirathi R, Mohan H. Studies on the aqueous extract of Terminalia chebula as a potent antioxidant and a probable radioprotector. Photomed 2004;11:530-8.
Chattopadhyay RR, Bhattacharyya SK. Plant Review Terminalia chebula. Pharm Rev 2007;23:145-50.
Chintalapani S, Swathi MS, Mangamoori LN. Phytochemical screening and in vitro antioxidant activity of whole plant extracts of Sesuvium portulacastrum. Asian J Pharm Clin Res 2018;11:322-7.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
Kuda T, Tsunekawa M, Goto H, Araki Y. Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. J Food Comp Anal 2005;18:625-33.
Tiwary AK, Puri M, Sharma D, Barrow CJ. Optimisation of novel method for the extraction of steviosides from Stevia rebaudiana leaves. Food Chem 2012;132:1113-20.
Bloknina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress. Ann Bot (Lond.) 2003;91:179-94.
Wani SM, Jan N, Wani TA, Ahmad M, Masoodi FA, Gani A. Optimization of antioxidant activity and total polyphenols of dried apricot fruit extracts (Prunus armeniaca L.) using response surface methodology. J Saudi Soc Agric Sci 2017;16:119-26.
Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 2002;8:121-37.
Jiang YH, Jiang XL, Bao HQ. Antioxidant Synergistic Effect and Formulation Optimization of Several Common Natural Pigments. ICMSA; 2015. p. 506-10.
Dakshayini PN, Mahaboob BP. Phytochemical screening and in vitro antioxidant potential of Tribulus terrestris fruit and Mesua ferrea flower extracts: A comparative study. Int J Pharm Pharm Sci 2018;10:70-5.
Moukette BM, Moor VJ, Nya CP, Nanfack P, Nzufo FT, Kenfack MA, et al. Antioxidant and synergistic antidiabetic activities of a three-plant preparation used in cameroon folk medicine. Hindawi Int Sch Res Notices 2017;2017:1-7.
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