CELL VIABILITY ASSAY OF FICUS BENGHALENSIS LATEX SOLVENT EXTRACTS ON DIFFERENT CELL LINES
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i10.26552Keywords:
Ficus benghalensis, Latex, Cell viability assay, Lymphocytes, Propidium iodide, Fluorescent microscopeAbstract
Objective: Presented here in the study, the screening for antiproliferative activity of Ficus benghalensis dried latex solvent extracts on human breast MDA MB 231, colorectal HCT116, and neuroblastoma IMR 32 cell lines.
Methods: The anticancer activity of ethanol, methanol, ethyl acetate, and acetone extracts against the above-mentioned cancer cell lines as well in lymphocytes, by 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and propidium iodide staining was used to observe the morphological changes occurred in the cell due to the affect of latex extract.
Results: Among all the extracts, ethanol extract was found to be effective against IMR 32 and HCT 116 whereas ethyl acetate extract in case of MDA MB 231 cell line with 50% inhibitory concentration 50% (IC50) 123.27±2.5 μg/ml, 99.82±9.06 μg/ml, and 75.66± 6.3, respectively.
Conclusion: The extracts were found to be less toxic on peripheral blood lymphocytes. The IC50 value of the cytotoxic activity measured using MTT dye indicated that the extracts were efficient in inhibition of the cell proliferation of these cell lines.
Downloads
References
Cragg GM, Newman DJ. Antineoplastic agents from natural sources: Achievements and future directions. Expert Opin Investig Drugs 2000;9:2783-97.
American Cancer Society. Cancer Facts and Figures. Georgia: American Cancer Society Inc.; 2010.
Amr A, Mousa M. Merits of anti-cancer plants from the Arabian Gulf region. Cancer Ther 2007;5:55-66.
Anand P, Sundaram C, Jhurani S, Kunnumakkara AB, Aggarwal BB. Curcumin and cancer: An old-age†disease with an age-old†solution. Cancer Lett 2008;267:133-64.
Campbell CT, Prince M, Landry GM, Kha V, Kleiner HE. Pro-apoptotic effects of 1’-acetoxychavicol acetate in human breast carcinoma cells. Toxicol Lett 2007;173:151-60.
Cao P, Cai X, Lu W, Zhou F, Huo J. Growth inhibition and induction of apoptosis in SHG-44 glioma cells by Chinese medicine formula Pingliu keliâ€. Evid Based Complement Alternat Med 2011;2011: pii: 958243.
Conforti F, Ioele G, Statti GA, Marrelli M, Ragno G, Menichini F, et al. Antiproliferative activity against human tumor cell lines and toxicity test on mediterranean dietary plants. Food Chem Toxicol 2008;46:3325-32.
Filomena C, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, et al. The protective ability of Mediterranean dietary plants against the oxidative damage: The role of radical oxygen species in inflammation and the polyphenol, flavonoid and sterol contents. Food Chem 2009;112:587-94.
Berquin IM, Edwards IJ, Chen YQ. Multi-targeted therapy of cancer by omega-3 fatty acids. Cancer Lett 2008;269:363-77.
Czene S, Testa E, Nygren J, Belyaev I, Harms-Ringdahl M. DNA fragmentation and morphological changes in apoptotic human lymphocytes. Biochem Biophys Res Commun 2002;294:872-8.
Subramanian PM, Misra GS. Chemical constituents of Ficus bengalensis (part II). Pol J Pharmacol Pharm 1978;30:559-62.
The wealth of India. In: A Dictionary of Indian Raw Materials and Industrial Products. Vol. 4. New Delhi: Council of Scientific and Industrial Research; 2005. p. 24-6.
Mukherjee PK, Saha K, Murugesan T, Mandal SC, Pal M, Saha BP, et al. Screening of anti-diarrhoeal profile of some plant extracts of a specific region of West Bengal, India. J Ethnopharmacol 1998;60:85-9.
Husain A, Virmani OP, Popli SP, Misra LN, Gupta MM, Srivastava GN, et al. Dictionary of Indian Medicinal Plants. Lucknow, India: CIMAP; 1992. p. 546.
Aiyer KN, Namboodiri AN, Kolammal M. Pharmacognosy of Ayurvedic Drugs. Pharmacognosy Unit. Trivandrum: Ayurveda College; 1957.
Gabhe SY, Tatke PA, Khan TA. Evaluation of the immunomodulatory activity of the methanol extract of Ficus benghalensis roots in rats. Indian J Pharmacol 2005;38:271-5.
Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants. Compendium of 500 Species. Madras: Orient Longman Ltd.; 1993- 1995. p. 1-5.
Shrotri DS, Aiman R. The relationship of the post-absorptive state to the hypoglycemic action studies on Ficus bengalensis and Ficus glomerata. Indian J Med Res 1960;48:162-8.
Deshmukh VK, Shrotri DS, Aiman R. Isolation of a hypoglycaemic principle from the bark of Ficus bengalensis Linn. Indian J Physiol Pharmacol 1960;4:182-5.
Augusti KT. Hypoglycaemic action of bengalenoside, a glucoside isolated from Ficus bengalensis Linn, in normal and alloxan diabetic rabbits. Indian J Physiol Pharmacol 1975;19:218-20.
Augusti KT, Daniel RS, Cherian S, Sheela CG, Nair CR. Effect of leucopelargonin derivative from Ficus bengalensis Linn. On diabetic dogs. Indian J Med Res 1994;99:82-6.
The Wealth of India. In: A Dictionary of Indian Raw Materials and Industrial Products. Vol. 4. New Delhi: Council of Scientific and Industrial Research; 1999. p. 24-6.
Subramanian PM, Misra GS. Chemical constituents of Ficus-bengalensis. Indian J Chem 1977;15:762.
Cherian S, Kumar RV, Augusti KT, Kidwai JR. Antidiabetic effect of a glycoside of pelargonidin isolated from the bark of Ficus bengalensis Linn. Indian J Biochem Biophys 1992;29:380-2.
Kumar RV, Augusti KT. Antidiabetic effect of a leucocyanidin derivative isolated from the bark of Ficus bengalensis linn. Indian J Biochem Biophys 1989;26:400-4.
Daniel RS, Devi KS, Augusti KT. Mechanism of action of antiatherogenic and related effects of Ficus bengalensis Linn. Flavonoids in experimental animals. Indian J Exp Biol 2003;41:296-303.
Chatterjee A. The treaties of Indian medicinal plants. ??? 1997;1:39.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Konstantinov SM, Eibl H, Berger MR. BCR-ABL influences the antileukaemic efficacy of alkylphosphocholines. Br J Haematol 1999;107:365-80.
Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl 1968;97:77-89.
Ullman SB, Halberstaedter L, Leibowitz J. Some pharmacological and biological effects of the latex of Ficus carica. Exp Med Surg 1945;3:11-23.
Ullman SB. The inhibitory and necrosis-inducing effects of the latex of Ficus carica L. On transplanted and spontaneous tumours. Exp Med Surg 1952;10:26-49.
Ullman SB, Clark GM, Roan KM. The effects of the fraction R3 of the latex of Ficus carica L. On the tissues of mice bearing spontaneous mammary tumors. Exp Med Surg 1952;10:287-305.
Ozçelik B, Kartal M, Orhan I. Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharm Biol 2011;49:396-402.
Tulasi C, Rani AS, Manjula B. Screening of phyto-chemicals, TLC profiling, total flavonoid and phenolics content, anti-oxidant activity and anti-microbial activity of Ficus benghalensis linn and Ficus religiosa linn latex. Int J Pharm Pharm Sci 2015;7:480-5.
Mathi P, Nikhil K, Das S, Roy P, Bokka VR, Botlagunta M. Evaluation of in vitro anticancer activity and Gc-Ms analysis from leaf Sophora interrupta bedd. Int J Pharm Pharm Sci 2015;7:303-8.
O’Brein MA, Kirby R. Apoptosis: A review of pro-apoptotic and anti-apoptotic pathways and dysregualtion in disease. J Vet Emerg Crit Care 2008;18:572-85.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
