ANTIOXIDANT ASSAY OF PHASEOLUS VULGARIS L BEANS ETHANOLIC EXTRACT IN VITRO AND IN VIVO

Dwi Rita Anggraini; Tri Widyawati; Mutiara Indah Sari; Henny Sri Wahyuni; Siti Syarifah; Ade Putra Fratamasinaga; Maya Anjelir Antika

doi:10.22159/ajpcr.2018.v11s1.26584

Authors

Dwi Rita Anggraini Department of Anatomy, Faculty of Medicine, Universitas Sumatera Utara, Jalan dr. Mansur, USU-Kampus, Medan 20155, Indonesia.
Tri Widyawati Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Sumatera Utara, Jalan dr. Mansur, USU-Kampus, Medan 20155, Indonesia.
Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Jalan dr. Mansur, USU-Kampus, Medan 20155, Indonesia.
Henny Sri Wahyuni Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Jalan Almamater, USUKampus, Medan 20155, Indonesia.
Siti Syarifah Department of Anatomy, Faculty of Medicine, Universitas Sumatera Utara, Jalan dr. Mansur, USU-Kampus, Medan 20155, Indonesia.
Ade Putra Fratamasinaga Department of Biomedical Science, Faculty of Medicine, Universitas Sumatera Utara, Jalan dr. Mansur, USU-Kampus, Medan 20155, Indonesia.
Maya Anjelir Antika Department of Biomedical Science, Faculty of Medicine, Universitas Sumatera Utara, Jalan dr. Mansur, USU-Kampus, Medan 20155, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26584

Keywords:

Antioxidant, Phaseoleus vulgaris L, Beans, Ethanolic extract, In vitro, In vivo

Abstract

Objectives: Antioxidant is a substance such as Vitamin C that able to remove potentially damaging oxidizing agents. Malondialdehyde (MDA) is an important marker of oxidative stress that have correlation with Type 2 diabetes mellitus (DM) progression. Phaseolus vulgaris L (PV) (beans) is one of the alternative medicines to treat DM in Indonesia. We investigated the antioxidant activity of ethanolic extract on PV (EEPV) beans using in vitro and in vivo model.

Methods: Antioxidant assay in vitro was conducted using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Alloxan (120 mg/kg, intraperitoneally)- induced diabetic rats were divided into 5 groups (n=5), i.e., NC: Normal control, P1: Diabetic-control, P2, P3, and P4 (200 mg/kg, 400 mg/kg, and 600 mg/kg of EEPV, orally) for 28 days. MDA serum level was evaluated using spectrophotometer. The data were analyzed by one-way ANOVA test.

Results: The antioxidant assay in vitro showed that EEPV has very weak (IC50=641.87 Î¼g/ml, whereas Vitamin C as standard showed very strong activity (IC50=2.95 Î¼g/ml). MDA level were 2.80Â±0.09 nmol/ml (NC), 3.30Â±0.42 nmol/ml (P1), 3.27Â±4.23 nmol/ml (P2), 2.93Â±0.05 nmol/ml (P3), and 3.17Â±0.25 nmol/ml (P4). There was no significant differences among normal control, diabetic-control, and EEPV-treated groups (p>0.05).

Conclusion: EEPV showed a very weak antioxidant activity in vitro.

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