MODIFICATED EXTRACTION AND PURITY TEST OF ARENGA PINNATA GUM

Jamaran Kaban; Julia Reveny; Juliati Tarigan; Nilsya Febrika Zebua

doi:10.22159/ajpcr.2018.v11s1.26593

Authors

Jamaran Kaban Department of Chemistry, Universitas Sumatera Utara, Medan, Indonesia.
Julia Reveny Department of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Juliati Tarigan Department of Chemistry, Universitas Sumatera Utara, Medan, Indonesia.
Nilsya Febrika Zebua Department of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26593

Keywords:

Galactomannan, Palm seed, Arenga pinnata, Modification, Extraction, Luff Schoorl

Abstract

Objective: The purpose of this study is modificated extraction method to obtain gum from Arenga pinnata Merr. and their purity test.

Methods: Gum has been extracted from palm seed using distilled water by centrifugation and warming. Characterization of gum was done by scanning electron microscopy-energy dispersivse X-ray and spectrophotometer infrared. Gum refluxed with HCl 2 N for 14 h would be the monomer. Galactose and mannose were used as standard. Purity test was done by the thin-layer chromatography (TLC) and Luff Schoorl method.

Results: The study results obtained that the modification methods (4.8%) and centrifugation (4.6%). Identification of functional groups with the spectrophotometer infrared provided the same spectrum forms between the two methods. Purity test by TLC with the best eluen butanol: ethanol:water 2:2:2. Luff Schoorl's method gives results of 87.46%, whereas the raw comparison galactose-mannose standard 96.69%.

Conclusions: The modification method gives more results with the same purity rate as the centrifugation method.

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