IDENTIFICATION GENE MUTATIONS RPOB CAUSE OF MULTIDRUG RESISTANCE TUBERCULOSIS IN HAJI ADAM MALIK HOSPITAL

Authors

  • Adhisty Nurpermatasari Department of Pharmacology, Faculty of Pharmacy Universitas Sumatera Utara, Medan, Indonesia.
  • Urip Harahap Department of Pharmacology, Faculty of Pharmacy Universitas Sumatera Utara, Medan, Indonesia.
  • Parluhutan Siagian Department of Haji Adam Malik Hospital, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26595

Keywords:

Multidrug resistance tuberculosis, Mutations, Gene rpoB, Polymerase chain reaction

Abstract

Objective: The study aimed to investigate gene mutations in Mycobacterium tuberculosis causing resistance to rifampicin (RIF) in multidrug resistance TB patients in Haji Adam Malik Hospital and determined the suitability of the general pattern of mutations that occur in patients with multidrug-resistant TB (MDR-TB) in the hospital with global mutation pattern.

Methods: This observational study was performed prospectively in 31 patients with MDR-TB who had data medical record results of GeneXpert MTB/ RIF positive for resistant to RIF period August–November 2016 in the hospital by taking sputum from patients. DNA extracted from sputum and then purified to be detected using polymerase chain reaction (PCR). PCR products were examined by electrophoresis.

Results: The highest percentage of 81 bp rpoB gene mutation MTB is called as RIF -resistance determining region which was located at codon 516 (100%) and then was followed by mutation at codon 531 (96.77%) and codon 526 (90.32%), respectively, while the smallest percentage of mutation (12.90%) MTB at codon was 533.

Conclusion: The rpoB gene shows a positive mutation-causing resistance to RIF.

Downloads

Download data is not yet available.

References

World Health Organization. Multidrug-Resistant Tuberculosis. Geneva, Switzerland: World Health Organization; 2015.

World Health Organization. Companion Handbook to the WHO Guidelines for the Programmatic Management of Drug-resistant Tuberculosis. Geneva: (WHO/HTM/TB/2014.11); 2014. p. 77-94.

World Health Organization. Global Tuberculosis Report. Geneva: (WHO/HTM/TB/2013.11); 2013. p. 6-67.

Rattan A, Kalia A, Ahmad N. Multidrug-resistant Mycobacterial tuberculosis: Molecular perspective. Emerg Infect Disease. 1998;4:195- 206.

Farnia P. Susceptibility Testing: Multiplex -PCR, PCR-SSCP and PCR–ELISA Mycobacteriology Research Center (MRC). Tehran:Iranian National Reference TB Laboratory, National Research Institute

of Tuberculosis & Lung Disease (NRITLD). Protocol No:2. 2012.

p. 21- 49.

Made DW, Wirajana IN, Yowani SC. Amplifikasi dan identifikasi mutasi

pada fragmen 0,5 KB gen rpoB Isolat 134 B Isolat 134 Mycobacterium

tuberculosis multidrug resistant dengan metode nested polymerase

chain reaction. J Kimia 2014;8:166-70.

Da Silva PE, Palomino JC. Molecular basis and mechanisms of drug

resistance in Mycobacterium tuberculosis: Classical and new drugs.

Antimicrob Chemother 2011;66:1417-30.

Zhang Y, Yew WW. Mechanisms of drug resistance in Mycobacterium

tuberculosis. Int J Tuber Lung Dis 2009;13:1320-30.

Lingala MA, Aparna S, Suman J, Rao MV, Ranganadha RP. Clinical

and geographical profiles of rpoB gene mutations in Mycobacterium

tuberculosis isolates from Hyderabad and Koraput in India. J Microbiol

Antimicrob 2010;2:13-8.

Yao C, Zhu T, Li Y. Detection of rpoB, katG and inhA mutations in

Mycobacterium tuberculosis in clinical isolates from chonqing as

determined by microarray. Clin Microbiol Infect 2010;16:1639-43.

Nghiem NM. Molecular characteristic of rifampisin and isonizid

resistant Mycobacterium tuberculosis strains isolated in vietnam. J Clin

Microbiol 2011;50:598-601.

Li J, Xin J, Zhang L, Jiang L, Cao H, Li L. Rapid detection of rpoB in

rifampin resistant M. tuberculosis from sputum samples by denaturing

gradient gel electrophoresis. Int J Med Sci 2012;9:148-56.

Castan P, Pablo A, Romero A. Point of care system for detection of

Mycobacterium tuberculosis and rifampin resistance in sputum

samples. J Clin Microb 2014;52:502-7.

Ubyaan R. Study of influence of RpoB gene mutation at codon 513;

Analysis on isolate papua. Proc Insinas 2012;195:1-5.

Coovadia YM, Mahomed S, Pillay M, Werner L, Mlisana K. rifampicin

mono-resistance in Mycobacterium tuberculosis in kwa zulu-natal,

South Africa: A significant phenomenon in a high prevalence TB-HIV

region. PLoS One 2013;8:e77712.

Published

26-04-2018

How to Cite

Nurpermatasari, A., U. Harahap, and P. Siagian. “IDENTIFICATION GENE MUTATIONS RPOB CAUSE OF MULTIDRUG RESISTANCE TUBERCULOSIS IN HAJI ADAM MALIK HOSPITAL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 155-8, doi:10.22159/ajpcr.2018.v11s1.26595.

Issue

Section

Original Article(s)

Most read articles by the same author(s)

<< < 1 2 3 > >>