ANTIOBESITY ACTIVITITY OF WATER FRACTIONS EXTRACT OF TAMARIND (TAMARINDUS INDICA L.) IN HIGH CARBOHYDRATE DIET INDUCED MALE WISTAR RATS

Nurul Hidayah; Ketut Adnyana I; Neng Fisheri; Finna Setiawan

doi:10.22159/ajpcr.2018.v11s1.26606

Authors

Nurul Hidayah Departement of Pharmacology and Toxicology, Faculty of Pharmacy, Polytechnic Health of Medan, Medan, Indonesia.
Ketut Adnyana I Departement of Pharmacology and Toxicology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia.
Neng Fisheri Departement of Pharmacology and Toxicology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia.
Finna Setiawan Departement of Pharmacology and Toxicology, University of Surabaya, Surabaya, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26606

Keywords:

Antiobesity, Fraction of water extract tamarind, High carbohydrate diet

Abstract

Objective: The prevalence of obesity increases each year globally. Multifactorial etiology of obesity requires therapy management including changing of diet and medicines. Some of obesity drugs have shown ineffectiveness and safety. The previous study showed that water extract of tamarind could reduce body weight (bw). This study aimed to test the activity fraction of water extract tamarind as antiobesity using high carbohydrate diet.

Method: The preventive research of antiobesity had done by given water fraction and ethyl acetate fraction of water extract tamarind following with induced high carbohydrate diet during 6th weeks in male Wistar rats. The parameters had observed including consumption of food, body weight, weight of feces, volume of urine, total cholesterols, triglycerides, blood glucose, index of organs, and accumulation of body fat.

Result: The ethyl acetate fraction at doses 4.5 mg/kg bw has shown significantly effect to decrease of total cholesterols level and decrease of triglycerides level at weeks 6 (p<0.05). All the tests of fraction have shown activity inhibition of increased body weight, decrease of appetite, total cholesterols, triglycerides, and blood glucose. Meanwhile, mechanism action of antiobesity as increase defecation, urination, and decrease index of organs and accumulation of body fat have not shown by all these test fractions.

Conclusion: The ethyl acetate fraction at doses of 4.5 mg/kg bw can inhibit raising of body weight, decrease of total cholesterols, and triglycerides level greater than the other test groups, where increasing of these levels of blood biochemistry was closely related to the pathology of obesity.

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References

Dipiro JT, Talbert RL, Yee GC, Matzhe GR, Wells BG, Posey BG. Pharmacotherapy a Pathophysiologic Approach. 7th ed. New York: Mc. Graw Hill; 2008. p. 2437-51.

Nguyen DM, El-Serag HB. The epidemiology of obesity. Gastroenterol Clin North Am 2011;39:1-7.

Sunyer FX. Why Drugs? Pharmacotheraphy of Obesity. Switzerland: Birkhauser Verlag; 2008.

Azman KF, Amom Z, Azlan A, Esa NM, Ali RM, Shah SM, et al. Antiobesity effect of Tamarindus indica L. pulp aquous extract in high fat diet-induced obese rats. J Nat Med 2011;66:333-42.

Kuru P. Tamarindus indica and its health related effects. Asian Pac J Trop Biomed 2014;4:751-7.

Finna S, Adnyana IK, Sukandar EY, Sukrasno S, Yuniarto A, Pebritrinasari R. Pharmacological screening for potency of antiobesity on five plants based on ethnopharmacological use. Asian J Pharm Clin Res 2015;8:234-6.

Finna S, Adnyana IK, Sukandar EY, Sukrasno S. Activity of Tamarindus indica pulp water extract in high carbohydrate diets as antiobesity. J Chin Pharm Sci 2016;25:829-97.

Iftekhar AS, Rayhan I, Quadir MA, Akhteruzzaman S, Hasnat A. Effect of Tamarindus indica fruits on blood pressure and lipid-profile in human model: An in vivo approach. Pak J Pharm Sci 2006;19:125-9.

Iswanti DP, Kosim LD, Susanti A. In vitro pancreatic lipase inhibition by water and ethanol extract of Tamarindus indica and Kaempferia rotunda. Indones J Nat 2011;13: ???.

Koyagura N, Hemanth V, Jamadar MG, Huilgol S, Nayaka N, Yendigeri SM, et al. Antidiabetic and hepatoprotective activities of Tamarindus indica fruit pulp in alloxan induced diabetic rats. Research Article. Int J Pharmacol Clin Sci 2013;2:33-40.

Martinello F, Soares SM, Franco JJ, Santos AC, Sugohara A, Garcia SB. Hypolipemic and antioxidant activities from Tamarindus indica L. pulp fruit extract in hypercholesterolemic hamsters. Food Chem Toxicol 2006;44:810-8.

Finna S, Adnyana IK, Sukandar EY, Sukrasno S, Pebritrinasari R. Animal Model of Obesity in Populations With Hight-Carbohydrate Diet. Kuala Lumpur, Malaysia: 12th International Congress on Obesity; 2014. p. 17-20.

Wang T, Ma X, Peng D, Zhang R, Sun X, Chen M, et al. Effects of obesity related genetic variations on visceral and subcutaneous fat distribution in a Chinese population. Sci Rep 2016;6:20691.

Fajriaty I, Adnyana IK, Fidrianny I. Acute and sub-chronic (28 days) repeated oral toxicity test of ethanol extract (Sapindusrakak DC.) Fruits in wistar rats. Int J Pharm Pharm Sci 2014;6:487-92.

Gaesser GA. Carbohydrate quantity and quality in relation to body mass index. J Am Diet Assoc 2007;107:1768-80.

Fried SK, Rao SP. Sugars, hypertriglyceridemia, and cardiovascular disease. Am J Clin Nutr 2003;78:873S-880S.

Park EJ, Hellerstein MK. Carbohydrate induced hypertriglyceridemia Historical perspective and review of biological mechanism. Am J Clin Nutr 2000;71:412.

Raeni SM, Vanstone C, Papamandjaris A. Comparison of effect dietary fat retriction with that of energy retriction on human lipid metabolism. Am J Clin Nutr 2001;73:262-7.

Kalaiselvi P. Biochemistry. 1st ed. Chennai: Tamil Nadu Textbook and Educational Services Corporation, University of Madras, Taramani Campus; 2005. p. 30, 74.

Walker R, Whittlesea C. Clinical Pharmacy and Therapeutics. 5th ed. Edinburgh: Churchill Livingstone; 2012. p. 389.